Use of Lucifer Yellow VS as a Label in Fluorescent Immunoassays Illustrated by the Determination of Albumin in Serum
1983 ◽
Vol 20
(4)
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pp. 213-216
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Keyword(s):
The use of a new label for fluoroimmunoassay is described. Lucifer yellow VS is a highly fluorescent vinyl sulphone dye which, under mild conditions, forms covalent bonds with amino and sulphydryl groups but is extremely stable in water. A large Stokes shift (110 nm) and an emission maximum at 540 nm give Lucifer yellow further advantages over the more commonly used labels. The use of the dye as a label has been demonstrated by developing a heterogeneous fluoroimmunoassay for human serum albumin. The fluoroimmunoassay gave comparable results to those obtained using a less specific colorimetric dye-binding assay (r = 0·97, n = 20). The advantages, limitations, and other potential uses of Lucifer yellow are discussed.
1993 ◽
Vol 82
(6)
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pp. 675-676
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1972 ◽
Vol 12
(1)
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pp. 483-489
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Keyword(s):
2008 ◽
Vol 193
(2-3)
◽
pp. 81-88
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Keyword(s):
1980 ◽
Vol 3
(2)
◽
pp. 299-308
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Keyword(s):
1983 ◽
Vol 130
(2)
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pp. 257-261
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Keyword(s):