The preservation of nerve cells in rat neostriatal slices maintained in vitro: A morphological study

We tried a new method of prostate cancer treatment by inducing in vitro differentiation which resulted in reduction of cancer cells growth. A protein kinase inhibitor, midostaurin's ability to trigger the human prostate cancer cell line, DU145 to segregate into nerve cells was studied. Midostaurin (100 nM) suppressed the growth of DU145 cells but without change in the number of dead cells. Midostaurin started to extend neurites on DU145 cells after 24 hours and differentiated into nerve cells by 72 hours. The microtubule was stabilized by tau protein and its mRNA expression showed time-dependent increase in midostaurin-treated DU145 cells. At the same time, the amount of acetylcholinesterase was also increased. The midostaurin-treated DU145 cells showed 40% less activity than control in the colony forming assay. The results suggests that midostaurin can induce differentiation of DU145 cells into nerve cells.

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In Vitro Cultivation of Nerve Cells as a Model for Studies on Nerve Cell—Virus Interactions

Viruses, Immunity, and Mental Disorders ◽

10.1007/978-1-4613-1799-9_21 ◽

1987 ◽

pp. 227-233

Author(s):

Richard J. Ziegler

Keyword(s):

Nerve Cell ◽

Nerve Cells ◽

In Vitro Cultivation ◽

Virus Interactions

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A Morphological Study of Biomimetic Approach for Pathological Calcification In Vitro

International Journal of Oral-Medical Sciences ◽

10.5466/ijoms.2.23 ◽

2004 ◽

Vol 2 (1) ◽

pp. 23-32

Author(s):

Emiko Matsumura

Keyword(s):

Morphological Study ◽

Biomimetic Approach ◽

Pathological Calcification

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The secretion of proteins in vitro from Xenopus oocytes and their accessory cells: a biochemical and morphological study

Development ◽

10.1242/dev.61.1.367 ◽

1981 ◽

Vol 61 (1) ◽

pp. 367-383

Author(s):

T. J. Mohun ◽

C. D. Lane ◽

A. Colman ◽

C. C. Wylie

Keyword(s):

Gel Electrophoresis ◽

Messenger Rna ◽

Morphological Study ◽

Xenopus Oocytes ◽

Secretory Proteins ◽

Xenopus Laevis Oocytes ◽

Follicular Cells ◽

Two Dimensional Gel Electrophoresis ◽

Accessory Cells

Protein secretion by Xenopus laevis oocytes and their surrounding follicular cells in vitro has been investigated using two-dimensional gel electrophoresis. Viable oocytes, devoid of follicle layers, were prepared by treatment with collagenase; they retain in full their capacity to synthesize, sequester and export secretory proteins following microinjection with heterologous messenger RNA. Both RNA-injected and normal cells export a large number of endogenous oocyte proteins and, as with heterologous secretory translation products, these proteins are found within the oocyte in a vesicle fraction. Electron microscopy indicates that secretion involves exocytotic release of cortical vesicle contents. The follicular cells themselves also seem to contribute a number of proteins to the incubation medium surrounding isolated oocytes, but the presence of follicle layers is not required for the export of endogenous oocyte proteins.

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In vitro study of chorionic and ectoplacental trophoblast differentiation in the mouse

Development ◽

10.1242/dev.34.3.633 ◽

1975 ◽

Vol 34 (3) ◽

pp. 633-644

Author(s):

Danièle Hernandez-Verdun ◽

Chantal Legrand

Keyword(s):

Electron Microscopy ◽

Morphological Study ◽

Light And Electron Microscopy ◽

In Vitro Study ◽

Capillary Network ◽

Vitro Study ◽

Trophoblast Cells ◽

Trophoblast Differentiation ◽

Somite Stage

Mouse chorioallantoic pre-placental structures alone or in association with the embryo were explanted during the 9th day of gestation (7-somite stage) and cultured in a static medium for 24 to 48 h. From the subsequent morphological study of trophoblast differentiation, using both light and electron microscopy, we draw the following conclusions. 1. The allantoic mesoderm cells migrate inside the trophoblastic population but they do not differentiate a capillary network and trophoblast cells phagocytose the existing foetal erythrocytes. 2. In the absence of allantoic mesoderm, chorionic trophoblast cells remain undifferentiated. 3. The development of the chorionic trophoblast is modified in that chorionic trophoblast cells fail to establish close junctions with ectoplacental trophoblast, and some chorionic cells initiate the formation of multinucleated syncytia. The genesis of these syncytia is discussed.

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An in-vitro morphological study of Q-switched neodymium/YAG laser trabeculotomy Venkatesh, S., Lee, W. R., Guthrie, S., Cruickshank, F. R., Foulds, W. S., Quigley, R. J., and Bailey, R. T. (Dept. Electronic and Electrical Engineering, Univ. College London, Torrington Place, London WCIE 7JE, England). Br. J. Ophthalmol. 70:89, 1986

American Journal of Ophthalmology ◽

10.1016/0002-9394(86)90181-9 ◽

1986 ◽

Vol 102 (2) ◽

pp. 289

Keyword(s):

Morphological Study ◽

Electrical Engineering ◽

Yag Laser ◽

Neodymium Yag Laser

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Heterogeneity in Use-Dependent Depression of Inhibitory Postsynaptic Potentials in the Rat Neostriatum In Vitro

Journal of Neurophysiology ◽

10.1152/jn.1997.77.1.427 ◽

1997 ◽

Vol 77 (1) ◽

pp. 427-434 ◽

Cited By ~ 9

Author(s):

Gabriele Radnikow ◽

Jutta Rohrbacher ◽

Ulrich Misgeld

Keyword(s):

Aminobutyric Acid ◽

Medium Spiny Neurons ◽

Stimulus Interval ◽

Postsynaptic Potentials ◽

Inhibitory Postsynaptic Potentials ◽

Spiny Neurons ◽

Neostriatal Slices ◽

Minimal Stimulation ◽

Paired Pulse Depression

Radnikow, Gabriele, Jutta Rohrbacher, and Ulrich Misgeld. Heterogeneity in use-dependent depression of inhibitory postsynaptic potentials in the rat neostriatum in vitro. J. Neurophysiol. 77: 427–434, 1997. “Minimal stimulation” was applied to evoke responses in an “all-or-none” fashion in presumed medium spiny neurons of rat neostriatal slices in the presence of antagonists for glutamatergic excitation. For comparison, responses were evoked in the same cells by compound stimulation. Bicuculline (30 μM) blocked responses evoked by minimal stimulation, indicating that they were γ-aminobutyric acid-A (GABAA)-receptor-mediated inhibitory postsynaptic potentials (IPSPs), whereas responses evoked by compound stimulation were only reduced in amplitude. Likewise, R(−)baclofen (1–20 μM) blocked IPSPs evoked by minimal stimulation in all but one cell. On the contrary, responses evoked by compound stimulation were always reduced in amplitude but never blocked. Paired-pulse depression (PPD) of averaged responses to minimal and compound stimulation was observed at a stimulus interval of 300 ms. The GABAB receptor antagonist CGP55845A (0.5 μM) had no effect on PPD evoked by compound stimulation but abolished PPD evoked by minimal stimulation. In a second set of experiments, the two stimulation paradigms were used to evoke responses in neostriatal slices continuously bathed in R(−)baclofen (10–20 μM). In R(−)baclofen a strong PPD was evoked by minimal and by compound stimulation. The amplitude of the response to compound stimulation increased on application of CGP55845A (0.5 μM). At the same time, PPD evoked by compound stimulation decreased. On the contrary, IPSP amplitude and PPD evoked by minimal stimulation remained unchanged. We conclude that two types of GABAergic terminals exist in the rat neostriatum, only one of which is regulated by GABAB receptors. However, the other class of terminals, not regulated by GABAB receptors, displays a much more pronounced PPD.

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