In Vitro Cultivation of Nerve Cells as a Model for Studies on Nerve Cell—Virus Interactions

1987 ◽  
pp. 227-233
Author(s):  
Richard J. Ziegler
Keyword(s):  
Nerve Cell ◽  
Nerve Cells ◽  
In Vitro Cultivation ◽  
Virus Interactions

scholarly journals An ex vivo murine model to study poliovirus-induced apoptosis in nerve cells

2002 ◽  
Vol 83 (8) ◽  
pp. 1925-1930 ◽  
Author(s):  
Thérèse Couderc ◽  
Florence Guivel-Benhassine ◽  
Viviane Calaora ◽  
Anne-Sophie Gosselin ◽  
Bruno Blondel
Keyword(s):  
Nerve Cell ◽  
Cell Cultures ◽  
Motor Neurons ◽  
Molecular Mechanisms ◽  
Ex Vivo ◽  
Cell Types ◽  
Nerve Cells ◽  
Paralytic Poliomyelitis ◽  
Induced Apoptosis

Paralytic poliomyelitis results from destruction of motor neurons owing to poliovirus (PV) replication. Using a mouse model, we have previously shown that PV kills neurons of the central nervous system (CNS) as a result of apoptosis (Girard et al., Journal of Virology 73, 6066–6072, 1999). We report the development of mixed mouse primary nerve cell cultures from the cerebral cortex of neonatal mice transgenic for the human PV receptor. These cultures contained all three main cell types of the CNS, i.e. neurons, astrocytes and oligodendrocytes. All three cell types were susceptible to PV infection and virus replication in the cultures led to DNA fragmentation characteristic of apoptosis. PV-induced apoptosis was inhibited by the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD.FMK), indicating that this process involved caspases. Thus, these mixed mouse primary nerve cell cultures are a new in vitro model for studying the molecular mechanisms of PV-induced apoptosis in nerve cells.

Borrelia persica: In vitro cultivation and characterization via conventional PCR and multilocus sequence analysis of two strains isolated from a cat and ticks from Israel

2015 ◽  
Vol 6 (6) ◽  
pp. 751-757 ◽  
Author(s):  
Sandra Schwarzer ◽  
Gabriele Margos ◽  
Evelyn Overzier ◽  
Volker Fingerle ◽  
Gad Baneth ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Multilocus Sequence Analysis ◽  
In Vitro Cultivation ◽  
Conventional Pcr

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals In Vitro Cultivation of the Syphilis Spirochete Treponema pallidum

2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Diane G. Edmondson ◽  
Steven J. Norris
Keyword(s):  
Treponema Pallidum ◽  
In Vitro Cultivation

scholarly journals What quantitative mechanical loading stimulates in vitro cultivation best?

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Jerry Natenstedt ◽  
Aimee C Kok ◽  
Jenny Dankelman ◽  
Gabrielle JM Tuijthof
Keyword(s):  
Mechanical Loading ◽  
In Vitro Cultivation

scholarly journals Acanthamoeba spp. as Agents of Disease in Humans

2003 ◽  
Vol 16 (2) ◽  
pp. 273-307 ◽  
Author(s):  
Francine Marciano-Cabral ◽  
Guy Cabral
Keyword(s):  
Cerebrospinal Fluid ◽  
Cutaneous Lesion ◽  
Laboratory Diagnosis ◽  
Immune Defense ◽  
In Vitro Cultivation ◽  
Cutaneous Lesions ◽  
Trophozoite Stage ◽  
Biopsy Material ◽  
Pathogenic Potential

SUMMARY Acanthamoeba spp. are free-living amebae that inhabit a variety of air, soil, and water environments. However, these amebae can also act as opportunistic as well as nonopportunistic pathogens. They are the causative agents of granulomatous amebic encephalitis and amebic keratitis and have been associated with cutaneous lesions and sinusitis. Immuno compromised individuals, including AIDS patients, are particularly susceptible to infections with Acanthamoeba. The immune defense mechanisms that operate against Acanthamoeba have not been well characterized, but it has been proposed that both innate and acquired immunity play a role. The ameba's life cycle includes an active feeding trophozoite stage and a dormant cyst stage. Trophozoites feed on bacteria, yeast, and algae. However, both trophozoites and cysts can retain viable bacteria and may serve as reservoirs for bacteria with human pathogenic potential. Diagnosis of infection includes direct microscopy of wet mounts of cerebrospinal fluid or stained smears of cerebrospinal fluid sediment, light or electron microscopy of tissues, in vitro cultivation of Acanthamoeba, and histological assessment of frozen or paraffin-embedded sections of brain or cutaneous lesion biopsy material. Immunocytochemistry, chemifluorescent dye staining, PCR, and analysis of DNA sequence variation also have been employed for laboratory diagnosis. Treatment of Acanthamoeba infections has met with mixed results. However, chlorhexidine gluconate, alone or in combination with propamidene isethionate, is effective in some patients. Furthermore, effective treatment is complicated since patients may present with underlying disease and Acanthamoeba infection may not be recognized. Since an increase in the number of cases of Acanthamoeba infections has occurred worldwide, these protozoa have become increasingly important as agents of human disease.

scholarly journals In Vitro Cultivation of the Hymenoptera Genetic Model, Nasonia

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e51269 ◽  
Author(s):  
Robert M. Brucker ◽  
Seth R. Bordenstein
Keyword(s):  
Genetic Model ◽  
In Vitro Cultivation
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