Temporal profile of nuclear DNA fragmentation in situ in gerbil hippocampus following transient forebrain ischemia

1995 ◽  
Vol 671 (2) ◽  
pp. 305-308 ◽  
Author(s):  
Tomohiko Iwai ◽  
Akira Hara ◽  
Masayuki Niwa ◽  
Masakatsu Nozaki ◽  
Toshihiko Uematsu ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Nuclear Dna ◽  
Forebrain Ischemia ◽  
Temporal Profile ◽  
Transient Forebrain Ischemia ◽  
Nuclear Dna Fragmentation

Hypothermic prevention of nuclear DNA fragmentation in gerbil hippocampus following transient forebrain ischemia

1995 ◽  
Vol 17 (6) ◽  
pp. 461-464 ◽  
Author(s):  
Akira Hara ◽  
Naoki Yoshimi ◽  
Hideki Mori ◽  
Tomohiko Iwai ◽  
Noboru Sakai ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Nuclear Dna ◽  
Forebrain Ischemia ◽  
Transient Forebrain Ischemia ◽  
Nuclear Dna Fragmentation

scholarly journals Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.

1992 ◽  
Vol 119 (3) ◽  
pp. 493-501 ◽  
Author(s):  
Y Gavrieli ◽  
Y Sherman ◽  
S A Ben-Sasson
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Dna Fragmentation ◽  
Nuclear Dna ◽  
Nuclear Periphery ◽  
Lymphoid Tissues ◽  
Pooled Dna ◽  
Nuclear Dna Fragmentation ◽  
Small And Large Intestine

Programmed cell death (PCD) plays a key role in developmental biology and in maintenance of the steady state in continuously renewing tissues. Currently, its existence is inferred mainly from gel electrophoresis of a pooled DNA extract as PCD was shown to be associated with DNA fragmentation. Based on this observation, we describe here the development of a method for the in situ visualization of PCD at the single-cell level, while preserving tissue architecture. Conventional histological sections, pretreated with protease, were nick end labeled with biotinylated poly dU, introduced by terminal deoxy-transferase, and then stained using avidin-conjugated peroxidase. The reaction is specific, only nuclei located at positions where PCD is expected are stained. The initial screening includes: small and large intestine, epidermis, lymphoid tissues, ovary, and other organs. A detailed analysis revealed that the process is initiated at the nuclear periphery, it is relatively short (1-3 h from initiation to cell elimination) and that PCD appears in tissues in clusters. The extent of tissue-PCD revealed by this method is considerably greater than apoptosis detected by nuclear morphology, and thus opens the way for a variety of studies.

scholarly journals In situ labeling of nuclear DNA fragmentation in normal oral epithelia and squamous cell carcinomas.

1994 ◽  
Vol 36 (1) ◽  
pp. 67-70 ◽  
Author(s):  
Yoshihiro Abiko ◽  
Tomoyuki Ohuchi ◽  
Hisomu Nakahata ◽  
Toshiyuki Sadaoka ◽  
Masami Kanda ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Squamous Cell ◽  
Nuclear Dna ◽  
Squamous Cell Carcinomas ◽  
Nuclear Dna Fragmentation ◽  
Oral Epithelia

scholarly journals Apoptotic cell death at tooth eruption in rat molar observed by in situ labeling of nuclear DNA fragmentation.

1995 ◽  
Vol 37 (6) ◽  
pp. 488-492 ◽  
Author(s):  
Yoshihiro Abiko ◽  
Hidetoshi Kanno ◽  
Jiro Arai ◽  
Michiko Nishimura ◽  
Masato Saitoh ◽  
...  
Keyword(s):  
Cell Death ◽  
Dna Fragmentation ◽  
Nuclear Dna ◽  
Apoptotic Cell ◽  
Tooth Eruption ◽  
Apoptotic Cell Death ◽  
Nuclear Dna Fragmentation ◽  
Rat Molar

An in situ hybridization study on interleukin-1β mRNA induced by transient forebrain ischemia in the rat brain

1994 ◽  
Vol 26 (1-2) ◽  
pp. 135-142 ◽  
Author(s):  
Kazuki Yabuuchi ◽  
Masabumi Minami ◽  
Seishi Katsumata ◽  
Akira Yamazaki ◽  
Masamichi Satoh
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Interleukin 1Β ◽  
Forebrain Ischemia ◽  
Hybridization Study ◽  
Transient Forebrain Ischemia

scholarly journals Differences in nuclear DNA fragmentation and mitochondrial integrity of semen and prepared human spermatozoa

2000 ◽  
Vol 15 (7) ◽  
pp. 1552-1561 ◽  
Author(s):  
Eilish T. Donnelly ◽  
Michael O'Connell ◽  
Neil McClure ◽  
Sheena E.M. Lewis
Keyword(s):  
Dna Fragmentation ◽  
Nuclear Dna ◽  
Human Spermatozoa ◽  
Mitochondrial Integrity ◽  
Nuclear Dna Fragmentation
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