Effect of free fatty acid anion on the in vitro binding of 1-anilino-8-naphthalene sulfonate to cytoplasmic proteins from rat liver

1979 ◽  
Vol 24 (1) ◽  
pp. 83-93 ◽  
Author(s):  
Saroj Chakrabarti ◽  
Louise Charest-Boule ◽  
Jules Brodeur
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Rat Liver ◽  
Acid Anion ◽  
In Vitro Binding ◽  
Cytoplasmic Proteins ◽  
Vitro Binding ◽  
Fatty Acid Anion

Uncoupling activity of endogenous free fatty acids in rat liver mitochondria

1978 ◽  
Vol 56 (2) ◽  
pp. 111-116 ◽  
Author(s):  
Samuel H. P. Chan ◽  
Edward Higgins Jr.
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Rat Liver ◽  
Respiratory Control ◽  
Aging Effect ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria

Changes in the respiratory control index (RCI) and ADP:O ratio were found to be related to alterations in the free fatty acids levels of rat liver mitochondria aging in 0.25 M sucrose–Tris buffer at 0 °C. Free fatty acid levels increased with time after isolation of mitochondria while a concomitant decrease in the RCI and ADP:O ratio occurred. The changes in free fatty acid levels corresponded with the reported increasing levels of phospholipase A activity in aged mitochondrial preparations. Washing these mitochondria with sucrose buffer containing 1% defatted bovine serum albumin (BSA) counteracted the aging effect on the RCI (e.g., 2.5 to 3.5) and reduced the free fatty acid levels (e.g., 50 to 16 nmol/mg protein). This reversible phenomenon could be repeated several times during the in vitro aging at 0 °C. Use of 125I-iodinated BSA showed that approximately 5 μg BSA/mg mitochondria was adsorbed by the mitochondrial membranes during washing. These results indicate a direct correlation between the level of endogenous fatty acids and the uncoupling of mitochondrial oxidative phosphorylation. The mechanism of counteracting the aging effect by BSA involves the removal of some of the free fatty acids.

In vitro binding of3H-acrolein to regenerating rat liver DNA polymerase

1974 ◽  
Vol 30 (11) ◽  
pp. 1234-1236 ◽  
Author(s):  
Nicole Munsch ◽  
Anne-Marie de Recondo ◽  
C. Frayssinet
Keyword(s):  
Dna Polymerase ◽  
Rat Liver ◽  
In Vitro Binding ◽  
Regenerating Rat Liver ◽  
Vitro Binding
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