Differences in in vitro binding of diazepam and N-desmethyldiazepam to maternal and fetal plasma proteins at birth: Relation to free fatty acid concentration and other parameters

1983 ◽  
Vol 34 (2) ◽  
pp. 220-226 ◽  
Author(s):  
Wilhelm Kuhnz ◽  
Heinz Nau
2015 ◽  
Vol 17 (2) ◽  
pp. 92
Author(s):  
Lilis Hartati ◽  
Ali Agus ◽  
Lies Mira Yusiati ◽  
Budi Prasetyo Widyobroto

The aim of this study was to determine the levels of free fatty acids and carboxymethylcellulase activity (cmc-ase) activity of some protected fat-proteins base on in vitro Tilley and Terry method. Two sources of fat, i.e. crude palm oil and fish oil and three sources of protein i.e. skim milk, soybean flour and soybean meal were used in the formulation of protected fat-protein, and thus there were six treatment combinations. The filtrate from the in vitro test was analyzed for the levels of free fatty acids and  cmcase activity. The result of this research indicates that different combinations of feed materials and fat give different content of free fatty acid in first stage and second stage in vitro, with the best results in the combination treatment of skim milk and palm oil that give the lowest result of  free fatty acid concentration in fisrt stage in vitro (0.168%) and the highest result free fatty acid concentration in second stage in vitro ( 4.312%) . The activity of CMC-ase was not influenced by different  sources of fat and protein. It can be concluded was that the protection of the combination between skim milk and CPO gives the highest protection results.


1989 ◽  
Vol 8 (4) ◽  
pp. 156-158
Author(s):  
F. A. Müller ◽  
J. M. C. Oosthuizen ◽  
I. Reyneke

The separate and combined addition of insulin and melatonin to incubated liver slices produced an increase in the free fatty acid concentration in medium after incubation. Compared to insulin the addition of melatonin produced a more dramatic increase in medium free fatty acid concentration. The combined addition of insulin and melatonin have an additive effect on liver tissue in the production of free fatty acids. The increase in the free fatty acid concentration in incubation media is probably caused by stimulation of intracellular lipolysis by melatonin.


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