Cell position regulates endodermal differentiation in embryonal carcinoma cell aggregates

1983 ◽  
Vol 98 (1) ◽  
pp. 110-116 ◽  
Author(s):  
Maurice J. Rosenstraus ◽  
Joanne P. Spadoro ◽  
Joy Nilsson
Keyword(s):  
Embryonal Carcinoma ◽  
Carcinoma Cell ◽  
Embryonal Carcinoma Cell ◽  
Cell Aggregates ◽  
Cell Position ◽  
Endodermal Differentiation

Calcium-induced compaction and its inhibition in embryonal carcinoma cell aggregates

1983 ◽  
Vol 100 (1) ◽  
pp. 172-180 ◽  
Author(s):  
Sreedharan Kartha ◽  
Jeanette S. Felix ◽  
John W. Littlefield
Keyword(s):  
Embryonal Carcinoma ◽  
Carcinoma Cell ◽  
Embryonal Carcinoma Cell ◽  
Cell Aggregates

The mode of cell death associated with cavitation in teratocarcinoma-derived embryoid bodies

Development ◽  
1984 ◽  
Vol 80 (1) ◽  
pp. 63-74
Author(s):  
Sheilagh M. Boyd ◽  
M. L. Hooper ◽  
A. H. Wyllie
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Embryonal Carcinoma ◽  
Carcinoma Cell ◽  
Embryoid Bodies ◽  
Embryonal Carcinoma Cell ◽  
Embryonal Carcinoma Cell Line ◽  
Related Cell ◽  
Significant Difference ◽  
Tissue Modelling

Cell death occurring in embryoid bodies derived from the embryonal carcinoma cell line, PSA4, which undergo cavitation, and in those from the related cell line S2, which do not undergo cavitation, was classified as apoptosis or necrosis by ultrastructural criteria. Both modes of cell death were seen in PSA4 embryoid bodies while apoptosis alone was seen in S2 embryoid bodies. No significant difference was found between PSA4 and S2 embryoid bodies either in apoptotic incidence score or in the spatial distribution of apoptotic events. We therefore conclude that although apoptosis and tissue modelling coexist in PSA4 embryoid bodies, necrosis rather than apoptosis is causally related to formation of the cavity.

Expression of the Brachyury gene during mesoderm development in differentiating embryonal carcinoma cell cultures

Development ◽  
1994 ◽  
Vol 120 (1) ◽  
pp. 115-122 ◽  
Author(s):  
G. Vidricaire ◽  
K. Jardine ◽  
M.W. McBurney
Keyword(s):  
Bone Morphogenetic Proteins ◽  
Embryonal Carcinoma ◽  
Carcinoma Cell ◽  
Cell Aggregation ◽  
Cell Types ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cell ◽  
P19 Cells ◽  
Mesoderm Development ◽  
P19 Embryonal Carcinoma Cells

When aggregated and treated with dimethyl sulfoxide (DMSO), P19 embryonal carcinoma cells differentiate into cell types normally derived from the mesoderm and endoderm including epithelium and cardiac and skeletal muscle. The Brachyury gene is expressed transiently in these differentiating cultures several days before the appearance of markers of the differentiated cell types. The expression of Brachyury is not affected by DMSO but is induced by cell aggregation, which requires extracellular calcium. Expression of Brachyury is also induced by various members of the TGF beta family such as activin and bone morphogenetic proteins. D3 is a mutant clone of P19 cells selected for its failure to differentiate when aggregated in DMSO. Aggregated D3 cells express Brachyury mRNA suggesting that the mutation(s) responsible for the phenotype of D3 cells is downstream of the chain of events initiated by Brachyury expression.

STY, a tyrosine-phosphorylating enzyme with sequence homology to serine/threonine kinases

1991 ◽  
Vol 11 (1) ◽  
pp. 568-572
Author(s):  
B W Howell ◽  
D E Afar ◽  
J Lew ◽  
E M Douville ◽  
P L Icely ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nuclear Localization ◽  
Sequence Homology ◽  
Nuclear Localization Signal ◽  
Embryonal Carcinoma ◽  
Carcinoma Cell ◽  
Embryonal Carcinoma Cell ◽  
Embryonal Carcinoma Cell Line ◽  
Expression Product ◽  
Localization Signal

We have cloned a novel kinase (STY) from an embryonal carcinoma cell line. Sequence analysis of the STY cDNA reveals that it shares sequence homology with serine/threonine-type kinases and yet the bacterial expression product of the STY cDNA appears to have serine-, threonine-, and tyrosine-phosphorylating activities. The predicted STY protein is highly basic and contains a putative nuclear localization signal. During differentiation, two new mRNAs were detected in addition to the embryonic transcript.

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