Tritiated thymidine uptake by group G chromosomes of female individuals with Down's syndrome

In attempts to label the nuclei of intact amphibian embryos with tritiated thymidine we found, in agreement with other authors (Tencer, 1961; Quertier, 1962; Chibon, 1962), that when the nucleoside is dissolved in tap water or media of low salt concentration it fails to enter the embryo. However, when tritiated thymidine was either injected into the embryo or applied to isolated tissues immersed in salt solution, the marker diffused readily through the tissues. Evidently the outer surface of the amphibian embryo is furnished with a special permeability barrier which prevents or reduces the entrance of these molecules. This barrier corresponds most likely to what Holtfreter (1943a, 1943b) has described as the ‘surface coat’. In order that the results of our present experiments may be better understood some of the pertinent properties of the coat will be reviewed. A protective coat, while still absent in the ovarian egg, arises after the egg is deposited in water.

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Damage by Dichlorvos of Human Lymphocyte DNA

Tumori Journal ◽

10.1177/030089168006600403 ◽

1980 ◽

Vol 66 (4) ◽

pp. 425-430 ◽

Cited By ~ 5

Author(s):

Paolo Perocco ◽

Angela Fini

Keyword(s):

Dna Synthesis ◽

Human Lymphocyte ◽

Tritiated Thymidine ◽

Human Lymphocytes ◽

Ultraviolet Rays ◽

Thymidine Uptake ◽

Short Term ◽

In Vitro System ◽

Tritiated Thymidine Uptake

The action of dichlorvos (2.2-dichlorovinyldimethyl phosphate) was studied with a short-term in vitro system which utilizes human lymphocytes. The parameters studied were the action exerted by the pesticide on scheduled (semiconservative) and unscheduled (reparative) DNA synthesis measured as tritiated thymidine uptake. The results obtained show that dichlorvos affects semiconservative DNA synthesis, damages human lymphocyte DNA inducing low reparative synthesis, and interferes with DNA repair processes after damage exerted by ultraviolet rays.

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Comparative Studies of Chemotherapy of Human Tumor Cells In Vitro by Tritiated Thymidine Uptake Inhibition and Soft Agar Clonogenic Assay

The Journal of Urology ◽

10.1016/s0022-5347(17)45156-1 ◽

1986 ◽

Vol 136 (4) ◽

pp. 980-980

Author(s):

K.A. Zirvi ◽

G.J. Hill ◽

H.Z. Hill

Keyword(s):

Clonogenic Assay ◽

Tritiated Thymidine ◽

Human Tumor ◽

Soft Agar ◽

Thymidine Uptake ◽

Human Tumor Cells ◽

Uptake Inhibition ◽

Tritiated Thymidine Uptake ◽

Soft Agar Clonogenic Assay

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Vandellia Cordifolia Regulated Cell Proliferation and Cytokines Production in Human Mononuclear Cells

The American Journal of Chinese Medicine ◽

10.1142/s0192415x00000374 ◽

2000 ◽

Vol 28 (03n04) ◽

pp. 313-323 ◽

Cited By ~ 3

Author(s):

An-Pang Lin ◽

Wei-Jern Tsai ◽

Chi-Yen Fan ◽

Ming-Jen Lee ◽

Yuh-Chi Kuo

Keyword(s):

Cell Cycle Progression ◽

Mononuclear Cells ◽

Tritiated Thymidine ◽

Interleukin 2 ◽

Interferon Γ ◽

Thymidine Uptake ◽

Dependent Manner ◽

Human Mononuclear Cells ◽

Tritiated Thymidine Uptake ◽

Ifn Γ

Vandellia cordifolia (V. cordifolia) used for treatment inflammation in traditional Chinese medicine was selected for immunopharmacological activity test. The effects of V. cordifolia extracted fractions on human mononuclear cells (HMNC) proliferation were determined by tritiated thymidine uptake. The results indicated that VC-ME fraction suppressed HMNC proliferation activated with phytohemagglutinin (PHA) and stimulated cell cycle progression was arrested at the G0/G1 stage. The inhibitory mechanisms may involve the blocking of interleukin-2 (IL-2) and interferon-γ (IFN-γ) production, since VC-ME suppressed IL-2 and IFN-γ production of HMNC in a dose-dependent manner. Therefore, it is suggested that immunomodulatory agents are contained in V. cordifolia.

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