scholarly journals DNA sequences in a cloned restriction fragment containing the inverted repeat region from yeast 2 μm plasmid

FEBS Letters ◽  
1977 ◽  
Vol 82 (1) ◽  
pp. 121-124 ◽  
Author(s):  
J. Hindley
Keyword(s):  
Restriction Fragment ◽  
Dna Sequences ◽  
Inverted Repeat ◽  
Repeat Region ◽  
Inverted Repeat Region ◽  
2 Μm Plasmid

scholarly journals Functional analysis of a centromere from fission yeast: a role for centromere-specific repeated DNA sequences.

1990 ◽  
Vol 10 (5) ◽  
pp. 1863-1872 ◽  
Author(s):  
L Clarke ◽  
M P Baum
Keyword(s):  
Dna Sequences ◽  
Inverted Repeat ◽  
Repeated Sequence ◽  
Repeated Sequences ◽  
Central Core ◽  
Repeated Dna ◽  
Inverted Repeat Region ◽  
Repeated Dna Sequences ◽  
Centromere Function ◽  
Chromosome Ii

A circular minichromosome carrying functional centromere sequences (cen2) from Schizosaccharomyces pombe chromosome II behaves as a stable, independent genetic linkage group in S. pombe. The cen2 region was found to be organized into four large tandemly repeated sequence units which span over 80 kilobase pairs (kb) of untranscribed DNA. Two of these units occurred in a 31-kb inverted repeat that flanked a 7-kb central core of nonhomology. The inverted repeat region had centromere function, but neither the central core alone nor one arm of the inverted repeat was functional. Deletion of a portion of the repeated sequences that flank the central core had no effect on mitotic segregation functions or on meiotic segregation of a minichromosome to two of the four haploid progeny, but drastically impaired centromere-mediated maintenance of sister chromatid attachment in meiosis I. This requirement for centromere-specific repeated sequences could not be satisfied by introduction of random DNA sequences. These observations suggest a function for the heterochromatic repeated DNA sequences found in the centromere regions of higher eucaryotes.

Evolutionary stability of mitochondrial DNA organization in Achlya

1984 ◽  
Vol 62 (7) ◽  
pp. 571-576 ◽  
Author(s):  
David A. Boyd ◽  
Thomas C. Hobman ◽  
Sally A. Gruenke ◽  
Glen R. Klassen
Keyword(s):  
Mitochondrial Dna ◽  
Inverted Repeat ◽  
Restriction Site ◽  
Physical Map ◽  
Rrna Gene ◽  
Repeat Region ◽  
Inverted Repeat Region ◽  
Flip Flop ◽  
Unique Region ◽  
Achlya Klebsiana

A physical map of the mtDNA (mitochondrial DNA) of Achlya klebsiana has been constructed. The molecule is circular and has a size of 50.7 ± 1.3 kilobases (kb). A large portion of genome exists in the form of an inverted repeat region, each copy being from 9.74 to 10.92 kb in length. Part of the repeat region is homologous to the yeast mitochondrial 21S rRNA gene. Two isomers of the molecule are present owing to a flip–flop rearrangement of the unique regions. Since no restriction-site differences have been detected between the two repeat regions, it is likely that the inverted repeat condition promotes continual sequence conversion, thus preserving the evolutionary advantages of gene duplication. The physical map of A. klebsiana mtDNA was compared with that of Achlya ambisexualis E-87 and it was found that the inverted repeat regions, as well as a 7-kb unique region adjacent to one of the repeat regions, were highly conserved with respect to restriction-site spacing.

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