scholarly journals Determination of the free-energy difference of the adenine nucleotide translocator reaction in rat-liver mitochondria using intra- and extramitochondrial ATP-utilizing reactions

FEBS Letters ◽  
1981 ◽  
Vol 132 (2) ◽  
pp. 201-206 ◽  
Author(s):  
Ron J.A. Wanders ◽  
Albert K. Groen ◽  
Alfred J. Meijer ◽  
Joseph M. Tager
Keyword(s):  
Free Energy ◽  
Rat Liver ◽  
Adenine Nucleotide ◽  
Energy Difference ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Adenine Nucleotide Translocator ◽  
Free Energy Difference

scholarly journals The effect of butacaine on adenine nucleotide binding and translocation in rat liver mitochondria

1975 ◽  
Vol 148 (3) ◽  
pp. 527-531 ◽  
Author(s):  
D R Fayle ◽  
G J Barritt ◽  
F L Bygrave
Keyword(s):  
Rat Liver ◽  
Local Anaesthetic ◽  
Binding Sites ◽  
Adenine Nucleotide ◽  
Adenine Nucleotides ◽  
Nucleotide Binding ◽  
Local Anaesthetics ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Mitochondrial Inner Membrane

The effect of the local anaesthetic, butacaine, on adenine nucleotide binding and translocation in rat liver mitochondria partially depleted of their adenine nucleotide content was investigated. The range of butacaine concentrations that inhibit adenine nucleotide translocation and the extent of the inhibition are similar to the values obtained for native mitochondria. Butacaine does not alter either the total number of atractyloside-sensitive binding sites of depleted mitochondria, or the affinity of these sites for ADP or ATP under conditions where a partial inhibition of the rate of adenine nucleotide translocation is observed. The data are consistent with an effect of butacaine on the process by which adenine nucleotides are transported across the mitochondrial inner membrane rather than on the binding of adenine nucleotides to sites on the adenine nucleotide carrier. The results are briefly discussed in relation to the use of local anaesthetics in investigations of the mechanism of adenine nucleotide translocation.

scholarly journals Thyroid-hormone control of state-3 respiration in isolated rat liver mitochondria

1990 ◽  
Vol 265 (3) ◽  
pp. 731-734 ◽  
Author(s):  
R P Hafner ◽  
G C Brown ◽  
M D Brand
Keyword(s):  
Thyroid Hormone ◽  
Atp Synthase ◽  
Adenine Nucleotide ◽  
Liver Mitochondria ◽  
Proton Leak ◽  
Rat Liver Mitochondria ◽  
Protonmotive Force ◽  
Adenine Nucleotide Translocator ◽  
Hormone Control ◽  
Kinetics Of

Oxidative phosphorylation can be treated as two groups of reactions; those that generate protonmotive force (dicarboxylate carrier, succinate dehydrogenase and the respiratory chain) and those that consume protonmotive force (adenine nucleotide and phosphate carriers. ATP synthase and proton leak). Mitochondria from hypothyroid rats have lower rates of respiration in the presence of ADP (state 3) than euthyroid controls. We show that the kinetics of the protonmotive-force generators are unchanged in mitochondria from hypothyroid animals, but the kinetics of the protonmotive-force consumers are altered, supporting proposals that the important effects of thyroid hormone on state 3 are on the ATP synthase or the adenine nucleotide translocator.

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