scholarly journals Essential role of phosphatidylinositol 3-kinase in insulin-induced activation and phosphorylation of the cGMP-inhibited cAMP phosphodiesterase in rat adipocytes studies using the selective inhibitor wortmannin

FEBS Letters ◽  
1994 ◽  
Vol 350 (2-3) ◽  
pp. 314-318 ◽  
Author(s):  
Tova Rahn ◽  
Martin Ridderstråle ◽  
Hans Tornqvist ◽  
Vincent Manganiello ◽  
Gudrun Fredrikson ◽  
...  
Keyword(s):  
Essential Role ◽  
Selective Inhibitor ◽  
Phosphatidylinositol 3 Kinase ◽  
Camp Phosphodiesterase ◽  
Rat Adipocytes ◽  
Phosphatidylinositol 3

scholarly journals Role of phosphatidylinositol 3-kinase in oxidative stress-induced disruption of tight junctions. Vol. 278 (2003) 49239-49245

2004 ◽  
Vol 279 (7) ◽  
pp. 6204
Author(s):  
Parimal Sheth ◽  
Shyamali Basuroy ◽  
Chunying Li ◽  
Anjaparavanda P. Naren ◽  
Radhakrishna K. Rao
Keyword(s):  
Oxidative Stress ◽  
Tight Junctions ◽  
Phosphatidylinositol 3 Kinase ◽  
Phosphatidylinositol 3

scholarly journals Novel Role of Phosphatidylinositol 3-Kinase in CD28-mediated Costimulation

2000 ◽  
Vol 276 (12) ◽  
pp. 9003-9008 ◽  
Author(s):  
Yohsuke Harada ◽  
Eri Tanabe ◽  
Ryosuke Watanabe ◽  
Bonnie D. Weiss ◽  
Akira Matsumoto ◽  
...  
Keyword(s):  
Phosphatidylinositol 3 Kinase ◽  
Phosphatidylinositol 3

scholarly journals cAMP-stimulated Na+ transport in H441 distal lung epithelial cells: role of PKA, phosphatidylinositol 3-kinase, and sgk1

2004 ◽  
Vol 287 (4) ◽  
pp. L843-L851 ◽  
Author(s):  
Christie P. Thomas ◽  
Jason R. Campbell ◽  
Patrick J. Wright ◽  
Russell F. Husted
Keyword(s):  
Apical Cell ◽  
P38 Map Kinase ◽  
Lung Epithelial Cells ◽  
Epithelial Cell Line ◽  
Phosphatidylinositol 3 Kinase ◽  
Transport Pathway ◽  
Early Effect ◽  
Sustained Effect ◽  
Phosphatidylinositol 3

H441 cells, a bronchiolar epithelial cell line, develop a cAMP-regulated benzamil-sensitive Na+ transport pathway on permeable supports (Itani OA, Auerbach SD, Husted RF, Volk KA, Ageloff S, Knepper MA, Stokes JB, Thomas CP. Am J Physiol Lung Cell Mol Physiol 282: L631–L641, 2002). To understand the molecular basis for the stimulation of Na+ transport, we delineated the role of specific intracellular pathways and examined the effect of cAMP on αβγ-epithelial Na+ channel (ENaC) and sgk1 expression. Na+ transport increases within 5 min of cAMP stimulation and is sustained for >24 h. The sustained effect of cAMP on Na+ transport is abolished by LY-294002, an inhibitor of phosphatidylinositol 3-kinase, by H89, an inhibitor of PKA, or by SB-202190, an inhibitor of p38 MAP kinase. The sustained effect of cAMP was associated with increases in α-ENaC mRNA and protein but without a detectable increase in βγ-ENaC and sgk1. The early effect of cAMP on Na+ transport is brefeldin sensitive and is mediated via PKA. These results are consistent with a model where the early effect of cAMP is to increase trafficking of Na+ channels to the apical cell surface whereas the sustained effect requires the synthesis of α-ENaC.

Sign in / Sign up

Export Citation Format

Share Document