Determination of amphoretic surfactants in cosmetic cleansing products by high-performance liquid chromatography on a cation-exchange column

1995 ◽  
Vol 715 (1) ◽  
pp. 195-198 ◽  
Author(s):  
Angela Tegeler ◽  
Wolfgang Ruess ◽  
Erich Gmahl
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cation Exchange ◽  
High Performance ◽  
Exchange Column ◽  
Cation Exchange Column

Determination of haemoglobin A1c by liquid chromatography using a new cation-exchange column

2003 ◽  
Vol 791 (1-2) ◽  
pp. 73-83 ◽  
Author(s):  
Maria Beatriz de la Calle Guntiñas ◽  
René Wissiack ◽  
Guy Bordin ◽  
Adela Rosa Rodrı́guez
Keyword(s):  
Liquid Chromatography ◽  
Cation Exchange ◽  
Haemoglobin A1c ◽  
Exchange Column ◽  
Cation Exchange Column

scholarly journals DEVELOPMENT OF ANALYTICAL METHOD FOR THE DETERMINATION OF NINHYDRIN-POSITIVE SUBSTANCES IN AMINO ACIDS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

2018 ◽  
Vol 11 ◽  
Author(s):  
Srinivasa Rajagopalachari Nk ◽  
Shanmugasundaram P
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
High Performance ◽  
Hplc Method ◽  
Drug Products ◽  
Cation Exchange Column ◽  
The Individual

Aim and Objectives: The aim of the work is focused on the optimization of the high-performance liquid chromatography (HPLC) method for the determination of ninhydrin-positive substances in amino acids using HPLC technique in a single method. Since, most of the amino acids are widely used in the determination of Renal and Nutrition drug products exist independently in the monograph for each amino acid either by TLC or HPLC.Methods: The chromatographic separation was performed using sodium amino acid analysis cation exchange column using Sodium Eluent Na 315, Sodium eluent Na 425, and Sodium Eluent Na 640 as Mobile phase, performed by gradient program with detection of wavelength 570 nm using flow rate as 0.4 mL/min. The method has been evaluated using post-column derivatization technique (HPLC/Pinnacle PCX).Results: All the amino acids were eluted correspondingly at the individual retention time and the method shall be validated as per the ICH Q2R1 Guideline.Conclusion: The method has been successfully evaluated and developed for the analytical applications.

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