Determination of polyamines in hydrolysates of uremic plasma by high-performance cation-exchange column chromatography

Abstract A fast and simple method for determination of taurine in Infant formulas has been developed. The sample preparation uses disposable ultrafiltration cartridges to remove protein and clarify the sample. Hydrolysis Is avoided, simplifying the procedure and increasing efficiency. One mL sample Is centrlfuged In a cartridge for 45 mln. The filtrate Is diluted with pH 2.2 citrate buffer and Injected into a high performance amino acid analyzer. A cation-exchange column (sodium phase) Is used with a single buffer eluant and an Isocratic chromatographic program. Colorimetrlc detection is performed following post-column nlnhydrln reaction. Chromatographic resolution from other nlnhydrln-posltive compounds is excellent. Average recoveries for 3 levels of spike for various products were 100-102%. Precision Is 1-3% RSD, depending on product. Linearity, specificity, and ruggedness are excellent. The method Is applicable to quality control testing of milk-based, soy-based, and prehydrolyzed proteinbased Infant formulas In the ready-to-use, concentrate, and powder forms. A variety of commercially available Infant formulas from different manufacturers were analyzed and all were found to contain taurine levels comparable to human milk. Some human milk and cow's milk samples were also analyzed and results compare well with literature values

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High-performance liquid chromatographic analysis of platelet activating factor on a cation-exchange column by direct ultraviolet detection

Journal of Chromatography A ◽

10.1016/s0021-9673(01)83766-3 ◽

1986 ◽

Vol 363 (2) ◽

pp. 412-417 ◽

Cited By ~ 21

Author(s):

Nikolaos K. Andrikopoulos ◽

Constantinos A. Demopoulos ◽

Athanasia Siafaka-Kapadai

Keyword(s):

Cation Exchange ◽

Chromatographic Analysis ◽

High Performance ◽

Platelet Activating Factor ◽

High Performance Liquid Chromatographic ◽

Ultraviolet Detection ◽

Exchange Column ◽

Cation Exchange Column ◽

Liquid Chromatographic Analysis ◽

Liquid Chromatographic

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Determination of haemoglobin A1c by liquid chromatography using a new cation-exchange column

Journal of Chromatography B ◽

10.1016/s1570-0232(03)00202-2 ◽

2003 ◽

Vol 791 (1-2) ◽

pp. 73-83 ◽

Cited By ~ 18

Author(s):

Maria Beatriz de la Calle Guntiñas ◽

René Wissiack ◽

Guy Bordin ◽

Adela Rosa Rodrı́guez

Keyword(s):

Liquid Chromatography ◽

Cation Exchange ◽

Haemoglobin A1c ◽

Exchange Column ◽

Cation Exchange Column

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ANALYSIS FOR SMALL AMOUNTS OF CALCIUM, MAGNESIUM, BARIUM, AND SULPHATE USING PHTHALEIN PURPLE

Canadian Journal of Chemistry ◽

10.1139/v56-122 ◽

1956 ◽

Vol 34 (7) ◽

pp. 921-925 ◽

Cited By ~ 3

Author(s):

J. R. McCallum

Keyword(s):

Cation Exchange ◽

Distilled Water ◽

Complexometric Method ◽

Exchange Column ◽

Cation Exchange Column ◽

Calcium Magnesium

For the determination of sulphate ion in samples containing 3 to 20 mgm., it was found that the standard precipitation techniques did not give sufficiently accurate results, and were time consuming. Reference to the literature showed that with certain modifications a complexometric method proposed by Schwarzenbach etal. for samples containing 20 to 50 mgm. of sulphate was most likely to give accurate results rapidly. In this method the sample is passed through a cation exchange column and washed with distilled water into a flask. This solution is boiled and precipitated with excess standard disodium ethylenediaminetetracetate in the presence of alcohol and ammonia using phthalein purple indicator. Provided that the details of procedure described in this paper are closely adhered to, accuracy is usually within two per cent. Calcium, magnesium, and barium may also be titrated directly using the phthalein purple indicator.

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