Determination of haemoglobin A1c by liquid chromatography using a new cation-exchange column

2003 ◽  
Vol 791 (1-2) ◽  
pp. 73-83 ◽  
Author(s):  
Maria Beatriz de la Calle Guntiñas ◽  
René Wissiack ◽  
Guy Bordin ◽  
Adela Rosa Rodrı́guez
Keyword(s):  
Liquid Chromatography ◽  
Cation Exchange ◽  
Haemoglobin A1c ◽  
Exchange Column ◽  
Cation Exchange Column

scholarly journals Structural similarity of chymopapain forms as indicated by circular dichroism

1989 ◽  
Vol 257 (1) ◽  
pp. 183-186 ◽  
Author(s):  
S Solis-Mendiola ◽  
R Zubillaga-Luna ◽  
A Rojo-Dominguez ◽  
A Hernandez-Arana
Keyword(s):  
Liquid Chromatography ◽  
High Resolution ◽  
Cation Exchange ◽  
Proteolytic Activity ◽  
Structural Similarity ◽  
Tertiary Structures ◽  
Exchange Column ◽  
Cation Exchange Column ◽  
Modified Proteins ◽  
Circular Dichroïsm

Four chymopapain forms were isolated by high-resolution liquid chromatography on a cation-exchange column. The three major forms possess nearly identical secondary and tertiary structures, as judged from their c.d. spectra; these components showed similar proteolytic activity and Mr values close to that of papain. The fourth isolated component seems to be a mixture of modified proteins.

ANALYSIS FOR SMALL AMOUNTS OF CALCIUM, MAGNESIUM, BARIUM, AND SULPHATE USING PHTHALEIN PURPLE

1956 ◽  
Vol 34 (7) ◽  
pp. 921-925 ◽  
Author(s):  
J. R. McCallum
Keyword(s):  
Cation Exchange ◽  
Distilled Water ◽  
Complexometric Method ◽  
Exchange Column ◽  
Cation Exchange Column ◽  
Calcium Magnesium

For the determination of sulphate ion in samples containing 3 to 20 mgm., it was found that the standard precipitation techniques did not give sufficiently accurate results, and were time consuming. Reference to the literature showed that with certain modifications a complexometric method proposed by Schwarzenbach etal. for samples containing 20 to 50 mgm. of sulphate was most likely to give accurate results rapidly. In this method the sample is passed through a cation exchange column and washed with distilled water into a flask. This solution is boiled and precipitated with excess standard disodium ethylenediaminetetracetate in the presence of alcohol and ammonia using phthalein purple indicator. Provided that the details of procedure described in this paper are closely adhered to, accuracy is usually within two per cent. Calcium, magnesium, and barium may also be titrated directly using the phthalein purple indicator.

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