Studies on the protein metabolism of Culex pipiens L.—IV. Separation of free amino acids and peptides in adult mosquitoes by column chromatography

1963 ◽  
Vol 9 (4) ◽  
pp. 453-462 ◽  
Author(s):  
P.S. Chen
Keyword(s):  
Amino Acids ◽  
Column Chromatography ◽  
Protein Metabolism ◽  
Free Amino Acids ◽  
Free Amino ◽  
Culex Pipiens

Free Amino Acids in Human Saliva

1973 ◽  
Vol 52 (3) ◽  
pp. 418-419 ◽  
Author(s):  
Pan H. Papanayotou ◽  
Joanna Dozi-Vassiliades ◽  
Anast. Kovatsis
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Column Chromatography ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Human Saliva ◽  
Chromatographic Techniques

By the use of thin layer chromatographic techniques and exchange column chromatography, 20 free amino acids were found in human saliva. No qualitative or semiquantitative differences in the amino acid content were observed among caries-susceptible and caries-resistant individuals 20 to 30 years old.

The costs of cell volume regulation: protein metabolism during hyperosmotic adjustment

1992 ◽  
Vol 72 (3) ◽  
pp. 569-578 ◽  
Author(s):  
A. J. S. Hawkins ◽  
T. J. Hilbish
Keyword(s):  
Amino Acids ◽  
Cell Volume ◽  
Protein Metabolism ◽  
Free Amino Acids ◽  
Volume Regulation ◽  
Free Amino ◽  
Cell Volume Regulation ◽  
Amino Nitrogen ◽  
Body Protein ◽  
Metabolic Pool

To resolve the sources of amino nitrogen which is accumulated as intracellular solute during hyperosmotic volume regulation, components of protein metabolism were monitored during compensation for a change from 15 to 30% salinity in the blue mussel, Mytilus edulis L. Net solute gain stemmed primarily from a marked reduction in total output from the metabolic pool of free amino acids, most of this ‘saving’ resulting from slower whole-body protein synthesis, and the remainder from lower nitrogenous excretion. Indeed, total inputs to the metabolic pool of free amino acids actually decreased over the period of net solute gain at 30%. Associated contributions from dietary assimilation, de novo synthesis and the direct uptake of dissolved amino acids were each negligible, indicating that breakdown products from endogenous body protein were the only significant source of amino nitrogen accumulated during hyperosmotic regulation. This accumulation represented more than 3% of soft-tissue protein nitrogen within the whole animal. We therefore impress that the excretion, during hyposaline adjustment, of all nitrogen previously accumulated as solute in response to equivalent hypersaline change, represents a major component cost of cell volume regulation, and which helps to explain stress and even mortality consequent upon what may be small but frequent fluctuations of salinity.

scholarly journals The Iodine Compounds of Thyroid and Plasma Studied by Column Chromatography

1956 ◽  
Vol 9 (4) ◽  
pp. 586 ◽  
Author(s):  
TH Kennedy ◽  
HD Purves
Keyword(s):  
Amino Acids ◽  
Column Chromatography ◽  
Relative Abundance ◽  
Free Amino Acids ◽  
Free Amino ◽  
Iodine Compounds

A system of Goluum chromat.ography suitable for the separation of the biologically important iodine compounds is described. Analysis of hydrolysed i;hyroid tissue shows the presence of thyroxine, 3,5,3'-tri-iodothyronine enT), 3,5-di-iodotyrosille (DIT)', mono-iodotyrosine (MIT), and other unidentified iodinecontaining compounds. The relative abundance of thyroxiue and TIT present. as free amino acids in 'the thyroid is different. from that of the same amino acids in protein combinat.ion. The amount of freo t.hyroxine is less thall tho free TIT, whereas in the oombined amino acids thyroxine is in excess of TIT.

A SURVEY OF AMINO ACID CONTENT OF THE UREDOSPORES OF SOME RACES OF WHEAT RUST (PUCCINIA GRAMINIS)

1960 ◽  
Vol 38 (2) ◽  
pp. 244-247 ◽  
Author(s):  
M. E. McKillican
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Column Chromatography ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Puccinia Graminis ◽  
Wheat Leaves ◽  
Wheat Rust

The free and combined amino acids of seven samples of wheat rust were analyzed quantitatively by paper and column chromatography and compared with the amino acids present in uninfected wheat leaves. All races studied contained different proportions of free amino acids.

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