Free Amino Acids in Human Saliva

1973 ◽  
Vol 52 (3) ◽  
pp. 418-419 ◽  
Author(s):  
Pan H. Papanayotou ◽  
Joanna Dozi-Vassiliades ◽  
Anast. Kovatsis
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Column Chromatography ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Human Saliva ◽  
Chromatographic Techniques

By the use of thin layer chromatographic techniques and exchange column chromatography, 20 free amino acids were found in human saliva. No qualitative or semiquantitative differences in the amino acid content were observed among caries-susceptible and caries-resistant individuals 20 to 30 years old.

A SURVEY OF AMINO ACID CONTENT OF THE UREDOSPORES OF SOME RACES OF WHEAT RUST (PUCCINIA GRAMINIS)

1960 ◽  
Vol 38 (2) ◽  
pp. 244-247 ◽  
Author(s):  
M. E. McKillican
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Column Chromatography ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Puccinia Graminis ◽  
Wheat Leaves ◽  
Wheat Rust

The free and combined amino acids of seven samples of wheat rust were analyzed quantitatively by paper and column chromatography and compared with the amino acids present in uninfected wheat leaves. All races studied contained different proportions of free amino acids.

THE METABOLISM OF YEAST SPORULATION: VI. CHANGES IN AMINO ACID CONTENT DURING SPOROGENESIS

1964 ◽  
Vol 10 (5) ◽  
pp. 623-631 ◽  
Author(s):  
C. Ramirez ◽  
J. J. Miller
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Proline Content ◽  
Sporulation Medium ◽  
Free Proline

During 6-day exposures of cells of Saccharomyces cerevisiae to acetate sporulation medium, the content of free amino acids declined to approximately one-third of that of vegetative cells, but proline was exceptional in that it increased conspicuously in amount. The content of combined amino acids also diminished to about one-third, ammonia was evolved, and amino acids (not including proline) passed out of the cells into the medium. When dihydroxyacetone replaced acetate in the sporulation medium, the results were similar except that the decline in content of free and combined amino acids was much greater, more ammonia was evolved, and only very small amounts of amino acids could be detected in the medium. Transfer of sporulated cells to growth medium led to an increase in the pool of free amino acids, except for proline, which declined in amount.In two other species of Saccharomyces the free proline content also increased on exposure to sporulation medium, but in Schizosaccharomyces pombe and Torulopsis famata no such increase was observed.

THE VENOM OF THE MUD-DAUBER WASPS: I. SCELIPHRON CAEMENTARIUM: PRELIMINARY SEPARATIONS AND FREE AMINO ACID CONTENT

1963 ◽  
Vol 41 (1) ◽  
pp. 1943-1948 ◽  
Author(s):  
Rod O'Connor ◽  
Wm. Rosenbrook Jr.
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Acid Content ◽  
Free Amino ◽  
Amino Acid Content ◽  
Electrical Excitation ◽  
Free Amino Acid Content ◽  
Quantitative Investigation ◽  
Layer Chromatography

Quantitative investigation of Sceliphron caementarium venom, obtained by electrical excitation of the wasp, has shown an unexpectedly small protein fraction of the dried venom. A few percent of the venom consists of the free amino acids histidine, methionine, and pipecolic acid. Three other components have been isolated by paper and thin-layer chromatography and are found to give weak ninhydrin reactions, in addition to certain color tests characteristic of alkaloids.

scholarly journals Free amino acids as phagostimulants in cricket nuptial gifts: support for the ‘Candymaker’ hypothesis

2009 ◽  
Vol 5 (2) ◽  
pp. 194-196 ◽  
Author(s):  
Stuart Warwick ◽  
Karim Vahed ◽  
David Raubenheimer ◽  
Stephen J Simpson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
High Performance ◽  
Amino Acid Content ◽  
Free Amino Acid Content ◽  
Nuptial Gifts

Nuptial gifts that are manufactured by the male are found in numerous insect species and some spiders, but there have been very few studies of the composition of such gifts. If, as has been proposed recently, nuptial gifts represent sensory traps, males will be selected to produce gifts that are attractive to females but such gifts will not necessarily provide the female with nutritional benefits (the ‘Candymaker’ hypothesis). We examined the free amino acid content of the spermatophylax of the cricket Gryllodes sigillatus (Orthoptera: Gryllidae) using high performance liquid chromatography (HPLC). The spermatophylax (dry weight) consisted of approximately 7 per cent free amino acids. The free amino acid composition was highly imbalanced, with a low proportion of essential amino acids (18.7%) and a high proportion of proline and glycine. The main free amino acids found in the spermatophylax appeared to act as phagostimulants: the duration of feeding on artificial gels by females was positively related to the free amino acid content of the gels. The results therefore suggest that males use free amino acids to ‘sweeten’ a relatively low-value food item. A possible function of glycine in inhibiting female movement is also proposed.

scholarly journals Changes in the Pree Amino Acids of the Blood of Blowfly Larvae At Metamorphosis

1956 ◽  
Vol 9 (3) ◽  
pp. 400 ◽  
Author(s):  
RH Hackman
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Free Amino Acid Content ◽  
Metabolic Significance

Blood from the larval, prepupal, and early pupal stages of Calliphora augttr' (F.) contains the same 18 free amino acids. In addition, hydroxyproline is present in larval and prepupal blood. The quantitative differenccs in the concent,ratiolls of these amino acids arc reported. Larval blood has the highest free amino acid content (6'6 mgjml) followcd by early pupal blood (4'6 mgjml) and prepnpal blood (3'3 mgjml). As the larva matures to the prepupa, the greatest decreases occm in the concentrations in the blood of alanine, glutamic acid, glutamine, glycine, proline, and tyrosine. The metabolic significance of these changes are discussed.

The Action of Periodic Acid and its Salts on Wool

1969 ◽  
Vol 39 (9) ◽  
pp. 851-858 ◽  
Author(s):  
A. Kantouch ◽  
A. Bendak
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Periodic Acid ◽  
Amino Acid Content ◽  
Controlling Factor ◽  
Organic Reactions ◽  
Reaction Conditions

In the present work, the amino acid content of periodate-oxidized wool is investigated. In general, amino acids in periodate-oxidized wool decrease to different degrees depending on their difference in reactivity. The controlling factor for the change of amino acids in periodate-oxidized wool is the periodate consumption, regardless of the reaction conditions. At a periodate consumption of 2.5 mmole/g wool, a decrease in amino acids ranging from 3% for arginine to 70% for serine and tryptophan is observed. The change in amino acids in wool is also discussed on the basis of organic reactions, and, by analogy, with reaction of periodate with free amino acids and peptides.

scholarly journals The Free-Amino-Acid Content in Six Potatoes Cultivars through Storage

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1322
Author(s):  
Anna Pęksa ◽  
Joanna Miedzianka ◽  
Agnieszka Nemś ◽  
Elżbieta Rytel
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Solanum Tuberosum L ◽  
Amino Acid Content ◽  
Potato Cultivar ◽  
Free Amino Acid Content

Potatoes of six cultivars (Solanum tuberosum L.) with red, purple, and yellow flesh were stored at 2 and 5 °C for 3 and 6 months, and the influence of these factors on the content of free amino acids was determined. The potato cultivar and storage time had the greatest impact on the free amino acid content. The tubers of red-fleshed (Rote Emma) and purple-fleshed (Blue Congo) potatoes contained over 28 mg/g DM of free amino acids, and the Blaue Annelise cultivar with purple flesh had over 18 mg/g DM. After 6 months, the highest increase in their content (by 36%) was recorded in tubers of the Fresco cultivar (yellow-fleshed). In the analysed potatoes, the content of alanine, proline, serine, γ-aminobutyric acid, and α-aminoadipic acid increased, while that of asparagine, aspartic acid, and glutamine decreased. Asparagine decreased to the greatest extent in “Blaue Annelise” potatoes (by 24%) and that of glutamine in tubers of Rote Emma and Vineta by 18%.

THE VENOM OF THE MUD-DAUBER WASPS: I. SCELIPHRON CAEMENTARIUM: PRELIMINARY SEPARATIONS AND FREE AMINO ACID CONTENT

1963 ◽  
Vol 41 (9) ◽  
pp. 1943-1948 ◽  
Author(s):  
Rod O'Connor ◽  
Wm. Rosenbrook Jr.
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Acid Content ◽  
Free Amino ◽  
Amino Acid Content ◽  
Electrical Excitation ◽  
Free Amino Acid Content ◽  
Quantitative Investigation ◽  
Layer Chromatography

Quantitative investigation of Sceliphron caementarium venom, obtained by electrical excitation of the wasp, has shown an unexpectedly small protein fraction of the dried venom. A few percent of the venom consists of the free amino acids histidine, methionine, and pipecolic acid. Three other components have been isolated by paper and thin-layer chromatography and are found to give weak ninhydrin reactions, in addition to certain color tests characteristic of alkaloids.

scholarly journals Amino acids through developmental stages of sunflower leaves

2013 ◽  
Vol 72 (1) ◽  
pp. 23-33 ◽  
Author(s):  
Nayan Roy ◽  
Subrata Laskar ◽  
Anandamay Barik
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Total Protein ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Developmental Stages ◽  
Amino Acid Content ◽  
Free Amino Acid Content ◽  
Protein Levels

Abstract The PICO-TAGanalysis of proteins revealed that 17 protein-bound and 18 free amino acids were present throughout the developmental stages of sunflower leaves. The total protein-bound amino acid content was much higher than total free amino acid content throughout the development of sunflower leaves. The contents of protein-bound and free amino acids as well as essential and non-essential ones displayed different patterns with leaf maturation, suggesting that total protein levels are poor predictors of the nutritive status of leaves.

Free amino acid content of lymphocytes nd granulocytes compared.

1982 ◽  
Vol 28 (8) ◽  
pp. 1758-1761 ◽  
Author(s):  
K Fukuda ◽  
Y Hirai ◽  
H Yoshida ◽  
T Nakajima ◽  
T Usui
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Free Amino Acid Content ◽  
Freezing And Thawing ◽  
Healthy Men

Abstract For extracting free amino acids from human leukocytes, we find that disruption of the cells by ultrasonication is more reliable than freezing and thawing. The amount of free amino acids extracted by the latter method depends on the temperature and duration of thawing. We extracted free amino acids from leukocytes of healthy men by ultrasonication and compared their concentrations in lymphocytes and granulocytes. The amino acid content of granulocytes significantly (p less than 0.001) exceeded that of lymphocytes. Of the amino acids extracted from granulocytes, 76% was taurine; for lymphocytes taurine, aspartic acid, and glutamic acid were predominant, respectively composing 44%, 17%, and 26% of the total. Taurine is proposed as an index of cell disruption.

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