Influence of experimental conditions and DNA repair ability on EMS-induced mutagenesis and DNA binding in Escherichia coli K12

1982 ◽  
Vol 92 (1-2) ◽  
pp. 15-27 ◽  
Author(s):  
G.R. Mohn ◽  
A.A. van Zeeland ◽  
B.W. Glickman
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Dna Binding ◽  
Experimental Conditions ◽  
Escherichia Coli K12 ◽  
Repair Ability ◽  
Induced Mutagenesis

The lacI Gene as a Target for Mutation in Transgenic Rodents and Escherichia coli

Genetics ◽  
1998 ◽  
Vol 148 (4) ◽  
pp. 1441-1451
Author(s):  
Johan G de Boer ◽  
Barry W Glickman
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Dna Binding ◽  
Transgenic Animals ◽  
Single Copy ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Large Database ◽  
Deletion Mutations ◽  
Laci Gene

Abstract The lacI gene has been used extensively for the recovery and analysis of mutations in bacteria with various DNA repair backgrounds and after exposure to a wide variety of mutagens. This has resulted in a large database of information on mutational mechanisms and specificity of many mutagens, as well as the effect of DNA repair background on mutagenicity. Most importantly, knowledge about the mutational sensitivity of the lacI gene is now available, yielding information about mutable nucleotides. This popularity and available knowledge resulted in the use of the lacI gene in transgenic rodents for the study of mutagenesis in mammals, where it resides in ~40 repeated copies. As the number of sequenced mutations recovered from these animals increases, we are able to analyze the sites at which mutations have been recovered in great detail and to compare the recovered sites between bacteria and transgenic animals. The nucleotides that code for the DNA-binding domain are nearly saturated with base substitutions. Even after determining the sequences of ~10,000 mutations recovered from the animals, however, new sites and new changes are still being recovered. In addition, we compare the nature of deletion mutations between bacteria and animals. Based on the nature of deletions in the animals, we conclude that each deletion occurs in a single copy of the gene.

scholarly journals Genomic analysis of DNA binding and gene regulation by homologous nucleoid-associated proteins IHF and HU in Escherichia coli K12

2011 ◽  
Vol 40 (8) ◽  
pp. 3524-3537 ◽  
Author(s):  
Ana I. Prieto ◽  
Christina Kahramanoglou ◽  
Ruhi M. Ali ◽  
Gillian M. Fraser ◽  
Aswin S. N. Seshasayee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Regulation ◽  
Dna Binding ◽  
Genomic Analysis ◽  
Escherichia Coli K12 ◽  
Associated Proteins

Nitrosamine-induced mutagenesis in Escherichia coli K12 (343/113)

1981 ◽  
Vol 89 (3) ◽  
pp. 209-215 ◽  
Author(s):  
T.K. Rao ◽  
B.E. Allen ◽  
W. Winton ◽  
W. Lijinsky ◽  
J.L. Epler
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli K12 ◽  
Induced Mutagenesis

The osmZ (bglY) gene encodes the DNA-binding protein H-NS (H1a), a component of the Escherichia coli K12 nucleoid

1990 ◽  
Vol 224 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Gerhard May ◽  
Petra Dersch ◽  
Martin Haardt ◽  
Anke Middendorf ◽  
Erhard Bremer
Keyword(s):  
Escherichia Coli ◽  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Escherichia Coli K12

scholarly journals The antimutagenic effect of monoterpenes against UV-irradiation-, 4NQO- and t-BOOH-induced mutagenesis in coli

2011 ◽  
Vol 63 (1) ◽  
pp. 117-128 ◽  
Author(s):  
Biljana Nikolic ◽  
Dragana Mitic-Culafic ◽  
Branka Vukovic-Gacic ◽  
Jelena Knezevic-Vukcevic
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Uv Irradiation ◽  
Mutagenic Effect ◽  
Evolutionary Conservation ◽  
Induced Mutations ◽  
Mutagenic Potential ◽  
E Coli ◽  
Antimutagenic Effect ◽  
Induced Mutagenesis

The aim of this work was to investigate the antimutagenic potential of monoterpenes from sage and basil in Escherichia coli. The mutagenic potential of monoterpenes was pre-screened with Salmonella/microsome reversion assay in strain TA100 and no mutagenic effect was detected. The antimutagenic potential against UV- 4NQO- and t-BOOH induced mutagenesis was evaluated in E. coli K12 and E. coli WP2 by reversion assays. The obtained results indicate that camphor and thujone reduce UV- and 4NQO-induced mutations; myrcene reduces t-BOOH-induced mutations, while eucalyptol and linalool reduce mutagenicity by all tested mutagens. Considering evolutionary conservation of DNA repair and antioxidative protection, the obtained results indicate that further antigenotoxicity studies should be undertaken in eukaryotes.

scholarly journals The Virulence Plasmid-Encoded impCAB Operon Enhances Survival and Induced Mutagenesis in Shigella flexneriafter Exposure to UV Radiation

1999 ◽  
Vol 67 (3) ◽  
pp. 1415-1423 ◽  
Author(s):  
Laura J. Runyen-Janecky ◽  
Mei Hong ◽  
Shelley M. Payne
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Nucleotide Sequence ◽  
Uv Radiation ◽  
Uv Irradiation ◽  
Shigella Flexneri ◽  
Virulence Plasmid ◽  
Repair System ◽  
Induced Mutagenesis ◽  
Dna Fragment

ABSTRACT Upon exposure to UV radiation, Shigella flexneri SA100 displayed survival and mutation frequencies comparable to those ofEscherichia coli AB1157, which contains a functional UmuDC error-prone DNA repair system. Survival of SA100 after UV irradiation was associated with the presence of the 220-kb virulence plasmid, pVP. This plasmid encodes homologues of ImpA and ImpB, which comprise an error-prone DNA repair system encoded on plasmid TP110 that was initially identified in Salmonella typhimurium, and ImpC, encoded upstream of ImpA and ImpB. Although the impBgene was present in representatives of all four species ofShigella, not all isolates tested contained the gene.Shigella isolates that lacked impB were more sensitive to UV radiation than isolates that containedimpB. The nucleotide sequence of a 2.4-kb DNA fragment containing the imp operon from S. flexneri SA100 pVP was 96% identical to the impoperon from the plasmid TP110. An SA100 derivative with a mutation in the impB gene had reduced survival following UV irradiation and less UV-induced mutagenesis relative to the parental strain. We also found that S. flexneri contained a chromosomally encoded umuDC operon; however, theumuDC promoter was not induced by exposure to UV radiation. This suggests that the imp operon but not theumuDC operon contributes to survival and induced mutagenesis in S. flexneri following exposure to UV radiation.

Effect of ruv mutations on recombination and DNA repair in Escherichia coli K12

1984 ◽  
Vol 194 (1-2) ◽  
pp. 303-309 ◽  
Author(s):  
Robert G. Lloyd ◽  
Fiona E. Benson ◽  
Claire E. Shurvinton
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Escherichia Coli K12
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