Enzyme studies on human blood. XI. The isolation and characterization of thromboplastic cell and plasma components

1952 ◽  
Vol 5 (2) ◽  
pp. 223-224
Author(s):  
Jack Bloom
Keyword(s):  
Human Blood ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of human blood platelet gelsolin

FEBS Letters ◽  
1984 ◽  
Vol 174 (1) ◽  
pp. 80-85 ◽  
Author(s):  
A. Olomucki ◽  
C. Hue ◽  
F. Lefébure ◽  
M. Coué
Keyword(s):  
Human Blood ◽  
Blood Platelet ◽  
Isolation And Characterization ◽  
Human Blood Platelet

scholarly journals Regenerative Translation of Human Blood-Vessel-Derived MSC Precursors

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
William C. W. Chen ◽  
Bruno Péault ◽  
Johnny Huard
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Human Blood ◽  
Current Status ◽  
Tissue Repair And Regeneration ◽  
Isolation And Characterization ◽  
Adventitial Cells ◽  
Preclinical Animal Models

Mesenchymal stem/stromal cells (MSCs) represent a promising adult progenitor cell source for tissue repair and regeneration. Their mysterious identityin situhas gradually been unveiled by the accumulating evidence indicating an association between adult multipotent stem/progenitor cells and vascular/perivascular niches. Using immunohistochemistry and fluorescence-activated cell sorting, we and other groups have prospectively identified and purified subpopulations of multipotent precursor cells associated with the blood vessels within multiple human organs. The three precursor subsets, myogenic endothelial cells (MECs), pericytes (PCs), and adventitial cells (ACs), are located, respectively, in the three structural tiers of typical blood vessels: intima, media, and adventitia. MECs, PCs, and ACs have been extensively characterized in prior studies and are currently under investigation for their therapeutic potentials in preclinical animal models. In this review, we will briefly discuss the identification, isolation, and characterization of these human blood-vessel-derived stem cells (hBVSCs) and summarize the current status of regenerative applications of hBVSC subsets.

Dendritic cell precursor populations of mouse blood: identification of the murine homologues of human blood plasmacytoid pre-DC2 and CD11c+ DC1 precursors

Blood ◽  
2003 ◽  
Vol 101 (4) ◽  
pp. 1453-1459 ◽  
Author(s):  
Meredith O'Keeffe ◽  
Hubertus Hochrein ◽  
David Vremec ◽  
Bernadette Scott ◽  
Paul Hertzog ◽  
...  
Keyword(s):  
Human Blood ◽  
Ex Vivo ◽  
Mouse Blood ◽  
Surface Phenotype ◽  
Isolation And Characterization ◽  
Tnf Α ◽  
Close Relationship ◽  
Factor Α ◽  
And Function

Immature and predendritic cells (pre-DCs) of human blood are the most readily accessible human DC sources available for study ex vivo. Murine homologues of human blood DCs have not been described. We report the isolation and characterization of 2 populations of precursor DCs in mouse blood. Mouse blood cells with the surface phenotype CD11cloCD11b−CD45RAhi closely resemble human plasmacytoid cells (or pre-DC2) by morphology and function. On stimulation with oligonucleotides containing CpG motifs (CpG), these cells make large amounts of type 1 interferons and rapidly develop into DCs that bear CD8, though they may be distinct from the CD8+ DCs in the unstimulated mouse. A second population of cells with the surface phenotype CD11c+CD11b+CD45RA− closely resembles the immediate precursors of pre-DC1, rapidly transforming into CD8− DCs after tumor necrosis factor-α (TNF-α) stimulation. These findings indicate the close relationship between human and mouse DCs, provided cells are obtained directly from equivalent source materials.

Isolation and Characterization of Human Blood Platelet mRNA and Construction of a cDNA Library in λgt11

1989 ◽  
Vol 61 (03) ◽  
pp. 448-453 ◽  
Author(s):  
Andreas N Wicki ◽  
Alfred Walz ◽  
Susan N Gerber-Huber ◽  
Roland H Wenger ◽  
Rolf Vornhagen ◽  
...  
Keyword(s):  
Human Blood ◽  
Blood Platelets ◽  
Blood Platelet ◽  
Cdna Expression Library ◽  
Expression Library ◽  
Purification Method ◽  
Isolation And Characterization ◽  
Reticulocyte Lysate ◽  
Human Blood Platelets

SummaryWe have developed a purification method for the isolation of platelet-specific poly (A+) RNA and demonstrated that human blood platelets, despite the absence of a nucleus, contain stable mRNA. The poly (A+) RNA was used to construct a platelet- specific cDNA expression library in λgtll. The platelet derivation of the purified mRNA was confirmed by identification of membrane glycoprotein Ib (GPIb) message by immunoprecipitation of rabbit reticulocyte lysate translation products with poly- and monoclonal antibodies against GPIbα and by sequencing of a GPIbα cDNA clone

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