Antigenic and symbiotic characterization of indigenous Rhizobium leguminosarum bv. tripolii recovered from root nodules of Trifolium pratense L. sown into subterranean clover pasture soils

1988 ◽  
Vol 20 (3) ◽  
pp. 267-274 ◽  
Author(s):  
B. Valdivia ◽  
M.H. Dughri ◽  
P.J. Bottomley
Keyword(s):  
Rhizobium Leguminosarum ◽  
Trifolium Pratense ◽  
Root Nodules ◽  
Subterranean Clover ◽  
Trifolium Pratense L ◽  
Clover Pasture

scholarly journals Mineral Composition of Red Clover under Rhizobium Inoculation and Lime Application in Acid Soil

2015 ◽  
Vol 43 (2) ◽  
pp. 554-560 ◽  
Author(s):  
Olivera STAJKOVIC-SRBINOVIC ◽  
Dušica DELIC ◽  
Nataša RASULIC ◽  
Dragan CAKMAK ◽  
Djordje KUZMANOVIC ◽  
...  
Keyword(s):  
Mineral Composition ◽  
Rhizobium Leguminosarum ◽  
Trifolium Pratense ◽  
Acid Soil ◽  
Red Clover ◽  
Dry Weight ◽  
Rhizobium Inoculation ◽  
Shoot Dry Weight ◽  
Trifolium Pratense L ◽  
Lime Application

In the present study the effects of Rhizobium inoculation and lime application on the mineral composition (N, P, K, Ca, Mg, Fe, Mn, Cu, Zn, B) of red clover (Trifolium pratense L.), in very acid soil were evaluated. Inoculation with Rhizobium leguminosarum bv. trifolii significantly increased shoot dry weight (SDW) of red clover plants (three times greater), as well as N, Mg, Fe, Mn and Cu contents in plants compared to the control. Application of lime and Rhizobium together, depending on the lime rate (3, 6 or 9 t ha-1 of lime) and the cut, increased SDW significantly, but decreased the contents of N, P, K, Mg, Mn, Zn and B in plants. Regardless of the changes, in all treatments in both cuts, contents of N, K, Ca, Mg, Mn and Zn in plants were among sufficiency levels (Mg content was elevated in the second cut), while Fe content was mainly high, as well as Cu (in the second cut). Contents of P and B in plants were somewhat lower than sufficiency levels, but above critical level. Therefore, red clover can be grown with satisfactory yield and mineral composition in acid soil with Rhizobium inoculation only, but the application of P and B fertilization is desirable.

scholarly journals The Response of Red Clover (Trifolium pratense L.) to Separate and Mixed Inoculations with Rhizobium leguminosarum and Azospirillum brasilense in Presence of Polycyclic Aromatic Hydrocarbons

2020 ◽  
Vol 17 (16) ◽  
pp. 5751
Author(s):  
Karolina Furtak ◽  
Karolina Gawryjołek ◽  
Anna Gałązka ◽  
Jarosław Grządziel
Keyword(s):  
Azospirillum Brasilense ◽  
Rhizobium Leguminosarum ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Shoot Biomass ◽  
Polycyclic Aromatic ◽  
Trifolium Pratense L ◽  
The Impact ◽  
Positive Effect ◽  
Root And Shoot Biomass

This study aimed to evaluate the impact of co-inoculation Rhizobium sp. and Azospirillum sp. on plant (Trifolium pratense L.) growth in the presence of polycyclic aromatic hydrocarbon (PAH) contamination (anthracene, phenanthrene, and pyrene). Eight strains from the genus Rhizobium leguminosarum bv. trifolii were selected for biotest analysis. Two methods of inoculation were used in the chamber experiment: (1) R. leguminosarum alone and (2) a combined inoculant (R. leguminosarum and Azospirillum brasilense). For comparison, non-contaminated controls were also used. The results demonstrated that co-inoculation of plants with Rhizobium and Azospirillum resulted in more root and shoot biomass than in plants inoculated with R. leguminosarum alone. The results indicated that application of a co-inoculation of bacteria from Rhizobium and Azospirillum species had a positive effect on clover nodulation and growth under the condition of PAH contamination.

scholarly journals Characterization of the Anomalous Infection and Nodulation of Subterranean Clover Roots by Rhizobium leguminosarum 1020

Microbiology ◽  
1985 ◽  
Vol 131 (12) ◽  
pp. 3287-3302 ◽  
Author(s):  
E. M. HRABAK ◽  
G. L. TRUCHET ◽  
F. B. DAZZO ◽  
F. GOVERS
Keyword(s):  
Rhizobium Leguminosarum ◽  
Subterranean Clover

scholarly journals Oestrogenic Isoflavone Content in Natural Strains of Subterranean Clover (Trifolium subterraneum L.) from Sardinia

2006 ◽  
Vol 1 (7) ◽  
pp. 1934578X0600100 ◽  
Author(s):  
Aldo Tava ◽  
Luciano Pecetti ◽  
Alessia Bertoli ◽  
Efisio Piano
Keyword(s):  
Trifolium Pratense ◽  
Red Clover ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Biochanin A ◽  
Lower Amount ◽  
Positive Role ◽  
Isoflavone Concentration ◽  
Trifolium Pratense L ◽  
Natural Strains

A collection of subterranean clover (Trifolium subterraneum L.) genotypes from Sardinia was evaluated for the content of oestrogenic isoflavones to assess differences in total and individual compounds. Daidzein, genistein, and their respective 4′-methoxy derivatives biochanin A and formononetin, were detected in leaves and quantified by GC/FID and GC/MS methods. A large variation among genotypes for individual compounds and for the total isoflavone concentration was observed. Total isoflavones were measured in the range 0.8-13.4 mg/g fresh weight (0.3-4.5% dry matter). Daidzein was usually present in lower amount compared to the other isoflavones. Genistein and biochanin A were found in most cases in higher concentration than formononetin. Phyto-oestrogens have been shown to have both oestrogenic and anti-oestrogenic properties, and their effects entail a positive role for the human health. T. subterraneum revealed the presence of higher concentration of isoflavones compared to other sources of these compounds, e.g. soybean (Glycine max (L.) Merrill) and red clover (Trifolium pratense L.). This study provided information on the biodiversity of the natural strains from Sardinia, emphasizing the importance of these genetic resources for their variation in the amount of total and individual isoflavones.

scholarly journals Molecular Characterization of pssCDE Genes of Rhizobium leguminosarum bv. trifolii strain TA1: pssD Mutant Is Affected in Exopolysaccharide Synthesis and Endocytosis of Bacteria

1998 ◽  
Vol 11 (11) ◽  
pp. 1142-1148 ◽  
Author(s):  
Jaroslaw Król ◽  
Jerzy Wielbo ◽  
Andrzej Mazur ◽  
Joanna Kopcinska ◽  
Barbara Lotocka ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Rhizobium Leguminosarum ◽  
Trifolium Pratense ◽  
Microscopic Analysis ◽  
Gene Products ◽  
Nitrogen Fixing ◽  
Infection Threads ◽  
Significant Homology ◽  
Symbiotic Phenotype

We have identified the three genes pssCDE in Rhizobium leguminosarum bv. trifolii TA1. Even though they were almost identical to earlier identified pssCDE genes of R. leguminosarum, they differed in gene lengths and gene overlaps. The predicted gene products of pssCDE genes shared significant homology to prokaryotic glycosyl transferases involved in exopolysaccharide synthesis. The Tn5 insertion in pssD created the nonmucoid mutant that induced non-nitrogen-fixing nodules. The microscopic analysis of the nodules, induced on Trifolium pratense by the pssD133 mutant, showed abnormally enlarged infection threads densely packed with bacteria, which were released from the infection threads in an unusual way. The symbiosomes were observed very rarely and the nodule remained almost empty. Symbiotic phenotype of the pssD133 suggested a correlation between this mutation and defective endocytosis of bacteria into nodule cells.

Environmental modulation of the pssTNOP gene expression in Rhizobium leguminosarum bv. trifolii

2004 ◽  
Vol 50 (3) ◽  
pp. 201-211 ◽  
Author(s):  
Jerzy Wielbo ◽  
Andrzej Mazur ◽  
Jarosław E Król ◽  
Małgorzata Marczak ◽  
Anna Skorupska
Keyword(s):  
Gene Expression ◽  
Rhizobium Leguminosarum ◽  
Trifolium Pratense ◽  
Gene Promoter ◽  
Dependent Manner ◽  
Wild Type ◽  
In Planta ◽  
Phosphate Availability ◽  
Trifolium Pratense L ◽  
Successful Establishment

Exopolysaccharide production by Rhizobium leguminosarum bv. trifolii is required for successful establishment of nitrogen-fixing symbiosis with clover (Trifolium pratense L.). Using plasmid-borne transcriptional fusions of promoters of pss genes with promoterless lacZ the effect of root exudate, phosphate, and ammonia on expression of pssT, pssN, pssO, and pssP genes in wild-type strain RtTA1 background was determined. A stimulating effect of these environmental factors on pssO and pssP gene expression was observed. The putative pssO gene promoter was determined to be a strong promoter within which the divergent nod-box element was identified. The pssO promoter was slightly inducible in a flavonoid-dependent manner in wild-type R. leguminosarum bv. trifolii strains RtTA1 and ANU843 and very weakly active in a mutant of strain ANU843 that lacks the regulatory nodD gene. The expression of pssO and pssP genes in planta was investigated using plasmid-borne pssO–gusA and pssP–gusA fusions under different phosphate availability to clover. The level of pssO–gusA fusion expression was shown to be dependent on phosphate concentration in the plant growth medium.Key words: Rhizobium leguminosarum, exopolysaccharide, nod box, transcriptional fusion.

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