Molecular Characterization of pssCDE Genes of Rhizobium leguminosarum bv. trifolii strain TA1: pssD Mutant Is Affected in Exopolysaccharide Synthesis and Endocytosis of Bacteria

We have identified the three genes pssCDE in Rhizobium leguminosarum bv. trifolii TA1. Even though they were almost identical to earlier identified pssCDE genes of R. leguminosarum, they differed in gene lengths and gene overlaps. The predicted gene products of pssCDE genes shared significant homology to prokaryotic glycosyl transferases involved in exopolysaccharide synthesis. The Tn5 insertion in pssD created the nonmucoid mutant that induced non-nitrogen-fixing nodules. The microscopic analysis of the nodules, induced on Trifolium pratense by the pssD133 mutant, showed abnormally enlarged infection threads densely packed with bacteria, which were released from the infection threads in an unusual way. The symbiosomes were observed very rarely and the nodule remained almost empty. Symbiotic phenotype of the pssD133 suggested a correlation between this mutation and defective endocytosis of bacteria into nodule cells.

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Molecular characterization and symbiotic importance of prsD gene of Rhizobium leguminosarum bv. trifolii TA1.

Acta Biochimica Polonica ◽

10.18388/abp.1998_4364 ◽

1998 ◽

Vol 45 (4) ◽

pp. 1067-1073 ◽

Cited By ~ 2

Author(s):

A Mazur ◽

J Wielbo ◽

J Król ◽

J Kopcińska ◽

B Lotocka ◽

...

Keyword(s):

Molecular Characterization ◽

Abc Transporters ◽

Rhizobium Leguminosarum ◽

Trifolium Pratense ◽

Microscopic Analysis ◽

Type I ◽

Secretion Systems ◽

Sds Page ◽

The Family ◽

Culture Supernatants

The prsD, prsE and orf3 genes of Rhizobium leguminosarum bv. trifolii strain TA1 encode the proteins which are significantly related to the family of bacterial ABC transporters type I secretion systems. The prsD:Km(r) mutant of strain TA1 induced non-nitrogen-fixing nodules on Trifolium pratense. Microscopic analysis of the nodules induced by prsD mutant did not reveal major abberations in the bacteroid appearance. The exopolysaccharide of prsD mutant was produced in increased amount and its level of polymerization was changed. SDS/PAGE of the proteins from the culture supernatants showed a lack of the 47-kDa protein in the culture of prsD mutant. Thus, PrsD may play a role in the export of this protein.

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Antigenic and symbiotic characterization of indigenous Rhizobium leguminosarum bv. tripolii recovered from root nodules of Trifolium pratense L. sown into subterranean clover pasture soils

Soil Biology and Biochemistry ◽

10.1016/0038-0717(88)90002-8 ◽

1988 ◽

Vol 20 (3) ◽

pp. 267-274 ◽

Cited By ~ 9

Author(s):

B. Valdivia ◽

M.H. Dughri ◽

P.J. Bottomley

Keyword(s):

Rhizobium Leguminosarum ◽

Trifolium Pratense ◽

Root Nodules ◽

Subterranean Clover ◽

Trifolium Pratense L ◽

Clover Pasture

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Morphological, Biochemical and Molecular Characterization of Twelve Nitrogen-Fixing Bacteria and Their Response to Various Zinc Concentration

Jundishapur Journal of Microbiology ◽

10.5812/jjm.9415 ◽

2014 ◽

Vol 7 (4) ◽

Cited By ~ 4

Author(s):

Mohammad Dadook ◽

Sedigheh Mehrabian ◽

Mitra Salehi ◽

Saeed Irian

Keyword(s):

Molecular Characterization ◽

Zinc Concentration ◽

Nitrogen Fixing ◽

Nitrogen Fixing Bacteria

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Molecular Characterization of the vanE Gene Cluster in Vancomycin-Resistant Enterococcus faecalis N00-410 Isolated in Canada

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.46.6.1977-1979.2002 ◽

2002 ◽

Vol 46 (6) ◽

pp. 1977-1979 ◽

Cited By ~ 23

Author(s):

D. A. Boyd ◽

T. Cabral ◽

P. Van Caeseele ◽

J. Wylie ◽

M. R. Mulvey

Keyword(s):

Gene Cluster ◽

Molecular Characterization ◽

Enterococcus Faecalis ◽

Open Reading Frame ◽

Vancomycin Resistant Enterococcus ◽

Reading Frame ◽

Significant Homology ◽

Vancomycin Resistant ◽

Enterococcus Gallinarum

ABSTRACT The vanE operon was characterized from Enterococcus faecalis N00-410 (MIC of vancomycin = 24 μg/ml). The organization of the vanE operon was identical to that of the vanC1 operon from Enterococcus gallinarum, with protein identities ranging from 46 to 63%. An open reading frame located downstream of the vanE operon showed significant homology to a number of integrase genes, all of which are located downstream of the chromosomal GMP synthase gene guaA.

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Genetic and molecular characterization of GAL83: its interaction and similarities with other genes involved in glucose repression in Saccharomyces cerevisiae.

Genetics ◽

10.1093/genetics/135.3.655 ◽

1993 ◽

Vol 135 (3) ◽

pp. 655-664 ◽

Cited By ~ 6

Author(s):

J R Erickson ◽

M Johnston

Keyword(s):

Saccharomyces Cerevisiae ◽

Protein Kinase ◽

Molecular Characterization ◽

Gene Product ◽

Regulatory Mechanism ◽

Glucose Repression ◽

Null Mutation ◽

Gene Products ◽

Gal Genes

Abstract Expression of the GAL genes of Saccharomyces cerevisiae is subject to glucose repression, a global regulatory mechanism that requires several gene products. We have isolated GAL83, one of these genes required for glucose repression. The sequence of the predicted Gal83 protein is homologous to two other yeast proteins, Sip1p and Sip2p, which are known to interact with the SNF1 gene product, a protein kinase required for expression of the GAL genes. High-copy clones of SIP1 and SIP2 cross-complement the GAL83-2000 mutation (as well as GAL82-1, a mutation in another gene involved in glucose repression), suggesting that these four genes may perform similar functions in glucose repression. Consistent with this hypothesis, a gal83 null mutation does not affect glucose repression, and only dominant or partially dominant mutations exist in GAL83 (and GAL82). Two other observations were made that suggests that GAL83 functions interdependently with GAL82 and REG1 (another gene involved in glucose repression) to effect glucose repression: 1) REG1 on a low-copy plasmid cross-complements GAL82-1 and GAL83-2000 mutations, and 2) all pairwise combinations of reg1, GAL82-1 and GAL83-2000 fail to complement one another. Such unlinked noncomplementation suggests that Gal83p, Gal82p and Reg1p may interact with one another. Possible roles for GAL83, GAL82 and REG1 are discussed in relation to SNF1, SIP1 and SIP2.

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Detection and molecular characterization of ascarid nematode infection (Toxascaris leonina and Toxocara cati) in captive Asiatic lions (Panthera leo persica)

Acta Parasitologica ◽

10.2478/s11686-012-0012-y ◽

2012 ◽

Vol 57 (1) ◽

Cited By ~ 7

Author(s):

Rahul Pawar ◽

Uthandaraman Lakshmikantan ◽

Shakir Hasan ◽

Anantula Poornachandar ◽

Sisinthy Shivaji

Keyword(s):

Sequence Analysis ◽

Molecular Characterization ◽

Ribosomal Rna ◽

Pcr Amplification ◽

Microscopic Analysis ◽

Toxocara Canis ◽

Nucleotide Sequence Analysis ◽

Toxocara Cati ◽

Toxascaris Leonina

AbstractThe objective of this study was to investigate the ascarid infection in Asiatic lions using scat samples, based on microscopic analysis, PCR amplification of the ITS-2 region of ribosomal DNA and sequence analysis of the amplicons. Microscopic analysis indicated the presence of eggs of Toxascaris leonina in eleven of the sixteen scat samples analysed and in one of these eleven scats eggs of Toxocara cati were also detected. In five of the scats eggs were not detectable. The presence of T. leonina in all the infected samples was also confirmed by PCR amplification of the ITS-2 of ribosomal RNA gene and five of these also showed amplicons corresponding to T. cati, respectively. Toxocara canis infection was not observed in any of the scat samples. Nucleotide sequence analysis of the ITS-2 region indicated 97% to 99% similarity with T. leonina and T. cati, respectively. To our knowledge, this is the first molecular characterization of ascarid infection in captive Asiatic lions from a zoological garden of India. This study also indicates that Asiatic lions are more prone to infection either with T. leonina or T. cati and the parasite is not host specific.

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