The participation of growth factors in simulating the quiescent, proliferate, and differentiate stages of rat granulosa cells grown in a serum-free medium

The aim of this study was to explore the direct action of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells cultured in serum-free medium. Progesterone and estradiol production was measured in the presence and absence of noradrenaline, dopamine or propranolol using radioimmunoassays; statistical analysis was performed by analysis of variance and Newman-Keul's multiple range test. Twenty-six women aged 31±3 years undergoing in vitro fertilization and embryo transfer for infertility treatment at University Women's Hospital, University of Tübingen, Germany, took part in this study. Noradrenaline significantly inhibited progesterone production by human granulosa cells in a dose-related manner at a concentration of 10−4–10−6 mol/l. Dopamine significantly stimulated estradiol secretion by granulosa cells in an inverse dose-related manner. Both effects were blocked by propranolol. The results suggest that catecholaminergic actions switch over the steroid production of human granulosa cells cultured in serum-free medium from progesterone to estradiol.

Download Full-text

Effect of Growth Factors on Growth of Bovine Parathyroid Cells in Serum-Free Medium

World Journal of Surgery ◽

10.1007/s002689900125 ◽

1996 ◽

Vol 20 (7) ◽

pp. 822-829 ◽

Cited By ~ 11

Author(s):

Gregory P. Sadler ◽

Derek L. Jones ◽

J. Stuart Woodhead ◽

Kieran Horgan ◽

Malcolm H. Wheeler

Keyword(s):

Growth Factors ◽

Serum Free Medium ◽

Free Medium ◽

Serum Free

Download Full-text

Clonal proliferation of rat tracheal epithelial cells in serum-free medium and their responses to hormones, growth factors and carcinogens

Carcinogenesis ◽

10.1093/carcin/7.12.2033 ◽

1986 ◽

Vol 7 (12) ◽

pp. 2033-2039 ◽

Cited By ~ 40

Author(s):

David G. Thomassen ◽

Umberto Saffiotti ◽

M.Edward Kaighn

Keyword(s):

Growth Factors ◽

Epithelial Cells ◽

Serum Free Medium ◽

Free Medium ◽

Serum Free ◽

Tracheal Epithelial Cells ◽

Clonal Proliferation ◽

Tracheal Epithelial

Download Full-text

Insulin-like growth factors, IGF-1, IGF-2 and somatomedin C trigger cell proliferation in mammalian epithelial cells cultured in a serum-free medium

Experimental Cell Research ◽

10.1016/0014-4827(82)90370-6 ◽

1982 ◽

Vol 142 (2) ◽

pp. 293-300 ◽

Cited By ~ 29

Author(s):

John R. Reddan ◽

Dorothy C. Dziedzic

Keyword(s):

Cell Proliferation ◽

Growth Factors ◽

Epithelial Cells ◽

Serum Free Medium ◽

Free Medium ◽

Somatomedin C ◽

Serum Free ◽

Cells Cultured ◽

Insulin Like Growth Factors

Download Full-text

Orientation and directed migration of cultured corneal epithelial cells in small electric fields are serum dependent

Journal of Cell Science ◽

10.1242/jcs.109.6.1405 ◽

1996 ◽

Vol 109 (6) ◽

pp. 1405-1414 ◽

Cited By ~ 9

Author(s):

M. Zhao ◽

A. Agius-Fernandez ◽

J.V. Forrester ◽

C.D. McCaig

Keyword(s):

Growth Factors ◽

Electric Field ◽

Electric Fields ◽

Single Cells ◽

Serum Free Medium ◽

Free Medium ◽

Corneal Epithelial ◽

Serum Free ◽

Directed Migration ◽

Serum Dependent

Reorientation and migration of cultured bovine corneal epithelial cells (CECs) in an electric field were studied. Electric field application was designed to model the laterally directed, steady direct current electric fields which arise in an injured corneal epithelium. Single cells cultured in media containing 10% foetal bovine serum showed significant galvanotropism, reorienting to lie perpendicular to electric field vector with a threshold field strength of less than 100 mV/mm. Cells cultured in serum-free medium showed no reorientation until 250 mV/mm. Addition of EGF, bFGF or TGF-beta 1 singly or in combination to serum free medium significantly restored the reorientation response at low field strengths. Both the mean translocation rate and directedness of cell migration were serum dependent. Cultured in medium with serum or serum plus added EGF, single cells showed obvious cathodal migration at 100 mV/mm. Increasing electric field strength enhanced the cathodal directedness of single cell migration. Supplementing serum free medium with growth factors restored the cathodal directed migration of single cells and highest directedness was found for the combination of EGF and TGF-beta 1. Corneal epithelial sheets also migrated towards the cathode in electric fields. Serum or individual growth factors stimulated CEC motility (randomly directed). Applied fields did not further augment migration rates but added a vector to stimulated migration. Electric fields which are present in wounded cornea interact with other environmental factors and may impinge on CECs migration during wound healing. Therapies which combine the application of growth factors and electric fields may be useful clinically.

Download Full-text