Genome sequence of tick-borne encephalitis virus (Western subtype) and comparative analysis of nonstructural proteins with other flaviviruses

Virology ◽  
1989 ◽  
Vol 173 (1) ◽  
pp. 291-301 ◽  
Author(s):  
Christian W. Mandl ◽  
Franz X. Heinz ◽  
Elisabeth Stöckl ◽  
Christian Kunz
Keyword(s):  
Comparative Analysis ◽  
Genome Sequence ◽  
Encephalitis Virus ◽  
Nonstructural Proteins ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

scholarly journals Complete Genome Sequence of a Low-Virulence Tick-Borne Encephalitis Virus Strain

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
G. Dobler ◽  
M. Bestehorn ◽  
M. Antwerpen ◽  
A. Överby-Wernstedt
Keyword(s):  
Genome Sequence ◽  
Ixodes Ricinus ◽  
Virus Strain ◽  
Complete Genome Sequence ◽  
Neurological Disease ◽  
Complete Genome ◽  
Encephalitis Virus ◽  
Natural Focus ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

We report here the complete genome sequence (GenBank accession no. KX268728) of tick-borne encephalitis strain HB171/11, isolated from an Ixodes ricinus tick from a natural focus where human neurological disease is rare. The strain shows unique characteristics in neuroinvasiveness and neurovirulence.

scholarly journals Comparative Analysis of NS5 Protein for Tick Borne Encephalitis Virus Strains in three Virus Subtypes

2019 ◽  
Vol 3 (6) ◽  
pp. 36-47
Author(s):  
U. V. Potapova ◽  
S. I. Feranchuk ◽  
S. I. Belikov ◽  
G. N. Leonova
Keyword(s):  
Comparative Analysis ◽  
Normal Mode ◽  
Normal Mode Analysis ◽  
Encephalitis Virus ◽  
Mode Analysis ◽  
Structural Protein ◽  
Polymerase Domain ◽  
Methyltransferase Domain ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

Non-structural protein 5 (NS5) of tick-borne encephalitis virus is an enzyme which is responsible for a copying of viral RNA, and it has a strong structural similarity to RNA polymerases of another RNA virus families. The strains of the virus are separated into three subtypes, which differ by specific mutations in virus proteins, including NS5 protein. The methods of structural bioinformatics allow to construct a model of NS5 protein for several strains of the virus.The paper presents the comparative analysis of sequences and structures of NS5 protein, for three subtypes of the tick-borne encephalitis virus. The segments of protein were identified where the highest difference between subtypes and within subtypes is observed. These segments, where most of the mutations are accumulated, are located in methyltransferase domain, in the inter-domain interface, and in the three subdomains of polymerase domain. The association between the locations of mutations in NS5 protein and the flexibility of a protein backbone was observed using normal mode analysis. Namely, the most important mutations are located in the parts of protein where the amplitude of synchronous oscillations estimated using normal mode analysis is the highest: in the second zinc binding pocket within polymerase domain, in the N-terminal extension within inter-domain interface, and around an active site of methyltransferase domain.

scholarly journals Characterization of a Siberian Virus Isolated from a Patient with Progressive Chronic Tick-Borne Encephalitis

2003 ◽  
Vol 77 (1) ◽  
pp. 25-36 ◽  
Author(s):  
T. S. Gritsun ◽  
T. V. Frolova ◽  
A. I. Zhankov ◽  
M. Armesto ◽  
S. L. Turner ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genomic Sequence ◽  
Encephalitis Virus ◽  
Plaque Morphology ◽  
Amino Acid Substitutions ◽  
Ns1 Protein ◽  
Nonstructural Proteins ◽  
Dimerization Domain ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

ABSTRACT A strain of Tick-borne encephalitis virus designated Zausaev (Za) was isolated in Siberia from a patient who died of a progressive (2-year) form of tick-borne encephalitis 10 years after being bitten by a tick. The complete genomic sequence of this virus was determined, and an attempt was made to correlate the sequence with the biological characteristics of the virus. Phylogenetic analysis demonstrated that this virus belongs to the Siberian subtype of Tick-borne encephalitis virus. Comparison of Za virus with two related viruses, a Far Eastern isolate, Sofjin, and a Siberian isolate, Vasilchenko, revealed differences among the three viruses in pathogenicity for Syrian hamsters, cytopathogenicity for PS cells, plaque morphology, and the electrophoretic profiles of virus-specific nonstructural proteins. Comparative amino acid alignments revealed 10 individual amino acid substitutions in the Za virus polyprotein sequence that were different from those of other tick-borne flaviviruses. Notably, the dimeric form of the Za virus NS1 protein migrated in polyacrylamide gels as a heterogeneous group of molecules with a significantly higher electrophoretic mobility than those of the Sofjin and Vasilchenko viruses. Two amino acid substitutions, T277→V and E279→G, within the NS1 dimerization domain are probably responsible for the altered oligomerization of Za virus NS1. These studies suggest that the patient from whom Za virus was isolated died due to increased pathogenicity of the latent virus following spontaneous mutagenesis.

TBE in South Korea

2019 ◽  
Author(s):  
Joon Young Song
Keyword(s):  
South Korea ◽  
Human Case ◽  
Encephalitis Virus ◽  
Surveillance Studies ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

Although no human case of tick-borne encephalitis (TBE) has been documented in South Korea to date, surveillance studies have been conducted to evaluate the prevalence of tick-borne encephalitis virus (TBEV) in wild ticks.

TBE in Slovakia

2019 ◽  
Author(s):  
Jana Kerlik
Keyword(s):  
Encephalitis Virus ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

The former Czechoslovak Republic was one of the first countries in Europe where the tick-borne encephalitis virus (TBEV) was identified.

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