A comparative analysis on the physicochemical properties of tick-borne encephalitis virus envelope protein residues that affect its antigenic properties

2017 ◽  
Vol 238 ◽  
pp. 124-132 ◽  
Author(s):  
Yu. S. Bukin ◽  
Yu. P. Dzhioev ◽  
S.E. Tkachev ◽  
I.V. Kozlova ◽  
A.I. Paramonov ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Physicochemical Properties ◽  
Envelope Protein ◽  
Encephalitis Virus ◽  
Virus Envelope ◽  
Protein Residues ◽  
Tick Borne Encephalitis ◽  
Antigenic Properties ◽  
Virus Envelope Protein ◽  
Tick Borne Encephalitis Virus

scholarly journals N-linked glycan in tick-borne encephalitis virus envelope protein affects viral secretion in mammalian cells, but not in tick cells

2013 ◽  
Vol 94 (10) ◽  
pp. 2249-2258 ◽  
Author(s):  
Kentaro Yoshii ◽  
Natsumi Yanagihara ◽  
Mariko Ishizuka ◽  
Mizuki Sakai ◽  
Hiroaki Kariwa
Keyword(s):  
Envelope Protein ◽  
Mammalian Cells ◽  
Molecular Mechanisms ◽  
Consensus Sequence ◽  
Encephalitis Virus ◽  
Secretory Process ◽  
Virus Envelope ◽  
Tick Borne Encephalitis ◽  
Virus Envelope Protein ◽  
Tick Borne Encephalitis Virus

Tick-borne encephalitis virus (TBEV) is a zoonotic disease agent that causes severe encephalitis in humans. The envelope protein E of TBEV has one N-linked glycosylation consensus sequence, but little is known about the biological function of the N-linked glycan. In this study, the function of protein E glycosylation was investigated using recombinant TBEV with or without the protein E N-linked glycan. Virion infectivity was not affected after removing the N-linked glycans using N-glycosidase F. In mammalian cells, loss of glycosylation affected the conformation of protein E during secretion, reducing the infectivity of secreted virions. Mice subcutaneously infected with TBEV lacking protein E glycosylation showed no signs of disease, and viral multiplication in peripheral organs was reduced relative to that with the parental virus. In contrast, loss of glycosylation did not affect the secretory process of infectious virions in tick cells. Furthermore, inhibition of transport to the Golgi apparatus affected TBEV secretion in mammalian cells, but not in tick cells, indicating that TBEV was secreted through an unidentified pathway after synthesis in endoplasmic reticulum in tick cells. These results increase our understanding of the molecular mechanisms of TBEV maturation.

Characterization of Japanese encephalitis virus envelope protein expressed by recombinant baculoviruses

Virology ◽  
1989 ◽  
Vol 173 (2) ◽  
pp. 674-682 ◽  
Author(s):  
Yoshiharu Matsuura ◽  
Michiko Miyamoto ◽  
Takanori Sato ◽  
Chiharu Morita ◽  
Kotaro Yasui
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Envelope Protein ◽  
Encephalitis Virus ◽  
Recombinant Baculoviruses ◽  
Virus Envelope ◽  
Virus Envelope Protein
Sign in / Sign up

Export Citation Format

Share Document