scholarly journals Defective RNAs of clover yellow mosaic virus encode nonstructural/coat protein fusion products

Virology ◽  
1991 ◽  
Vol 183 (2) ◽  
pp. 479-486 ◽  
Author(s):  
K.Andrew White ◽  
J.B. Brancroft ◽  
George A. Mackie
1996 ◽  
Vol 23 (4) ◽  
pp. 429 ◽  
Author(s):  
S Karunaratne ◽  
A Sohn ◽  
A Mouradov ◽  
J Scott ◽  
HH Steinbiss ◽  
...  

Wheat plants (Triticum aestivum cv. Hartog) were stably transformed with the bar gene and the gene encoding the barley yellow mosaic virus coat protein. Cultured immature wheat embryos were bombarded with tungsten particles coated with the pEmuPAT-cp construct. Fifteen regenerating 'PPT- resistant' plants were selected on medium containing phosphinothricin. Of these, 11 plants had both the bar and cp genes integrated into the wheat genome and two plants had only the bar gene. Transmission of the two genes to progeny of two independent plants was confirmed. The barley yellow mosaic virus coat protein was detected in both the parent and progeny plants; however, bar gene expression occurred only in the parent plants.


Nature ◽  
1962 ◽  
Vol 194 (4826) ◽  
pp. 408-409 ◽  
Author(s):  
H. AGRAWAL ◽  
M. CHESSIN ◽  
L. BOS

Plant Disease ◽  
1997 ◽  
Vol 81 (7) ◽  
pp. 817-820 ◽  
Author(s):  
Robert T. Sherwood

Incidence of six viruses was tested in white clover from 28 rotationally grazed pastures of Pennsylvania (PA), New York (NY), and Vermont (VT). Each of 17 PA pastures was sampled fall 1994, spring 1995, fall 1995, and spring 1996, and 10 pastures were sampled fall 1996. Each of five NY and six VT pastures was sampled spring and fall 1995 and 1996. Enzyme-linked immunosorbent assays (ELISA) were conducted for red clover vein mosaic virus (RCVMV), white clover mosaic virus (WCMV), alfalfa mosaic virus (AlMV), peanut stunt virus (PSV), clover yellow mosaic virus (CYMV), and the potyvirus group (POTY). RCVMV, WCMV, AlMV, and POTY were detected in 28, 28, 27, and 25 of the 28 pastures and in 67, 32, 30, and 7% of the 3,065 samples tested, respectively. PSV occurred at low to moderate levels in 11 PA pastures. PSV was rare in NY and was not detected in VT. CYMV was never found. Incidence of each virus varied significantly among pastures. For any given virus, there was not a significant variation in incidence among sampling dates within the NY-VT samples. RCVMV, WCMV, and POTY varied among dates within PA.


2009 ◽  
Vol 99 (5) ◽  
pp. 512-518 ◽  
Author(s):  
S. J. Wylie ◽  
R. A. C. Jones

Seven complete genomes and 64 coat protein gene sequences belonging to Bean yellow mosaic virus (BYMV) isolates from different continents were examined for evidence of genetic recombination using six different recombination-detection programs. In the seven complete genomes and a single complete genome of the related virus Clover yellow vein virus (ClYVV), evidence for eight recombination patterns was found by four or more programs, giving firm evidence of their presence, and five additional recombination patterns were detected by three or fewer programs, giving tentative evidence of their occurrence. When the nucleotide sequences of 64 BYMV and one ClYVV coat protein genes were analyzed, three firm recombination patterns were detected in 21 isolates (32%). With another six isolates (9%), tentative evidence was found for three further recombination patterns. Of the 19 firm or tentative recombination patterns detected within and between strain groups of BYMV, and with ClYVV, 12 involved a generalist group of isolates as a parent but none of the other BYMV groups acted as parents more than six times. These findings suggest that recombination played an important role in the evolution of BYMV strain groups that specialize in infecting particular groups of domesticated plants.


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