Age-related changes in glucose metabolizing enzymes in spleen, thymus, and pulmonary lavage cells from F344 rats

The rostral ventrolateral medulla (RVLM) is an area of the brain stem that contains diverse neural substrates that are involved in systems critical for physiological function. There is evidence that aging affects some neural substrates within the RVLM, although age-related changes in RVLM molecular mechanisms are not well established. The goal of the present study was to characterize the transcriptomic profile of the aging RVLM and to test the hypothesis that aging is associated with altered gene expression in the RVLM, with an emphasis on immune system associated gene transcripts. RVLM tissue punches from young, middle-aged, and aged F344 rats were analyzed with Agilent’s whole rat genome microarray. The RVLM gene expression profile varied with age, and an association between chronological age and specific RVLM gene expression patterns was observed [ P < 0.05, false discovery rate (FDR) < 0.3]. Functional analysis of RVLM microarray data via gene ontology profiling and pathway analysis identified upregulation of genes associated with immune- and stress-related responses and downregulation of genes associated with lipid biosynthesis and neurotransmission in aged compared with middle-aged and young rats. Differentially expressed genes associated with the complement system and microglial cells were further validated by quantitative PCR with separate RVLM samples ( P < 0.05, FDR < 0.1). The present results have identified age-related changes in the transcriptomic profile of the RVLM, modifications that may provide the molecular backdrop for understanding age-dependent changes in physiological regulation.

Download Full-text

Age-related changes in the activities of the amine metabolizing enzymes of rat eye

Journal of Pharmacy and Pharmacology ◽

10.1111/j.2042-7158.1985.tb05084.x ◽

1985 ◽

Vol 37 (5) ◽

pp. 357-361 ◽

Cited By ~ 5

Author(s):

H. Cao Danh ◽

M. Strolin Benedetti ◽

A. Mousset ◽

P. Pasquier Béchet

Keyword(s):

Metabolizing Enzymes ◽

Age Related ◽

Age Related Changes

Download Full-text

Age-related changes in bone mineral content and density in intact male F344 rats

Bone ◽

10.1016/s8756-3282(01)00636-6 ◽

2002 ◽

Vol 30 (1) ◽

pp. 125-130 ◽

Cited By ~ 28

Author(s):

J. Banu ◽

L. Wang ◽

D.N. Kalu

Keyword(s):

Bone Mineral Content ◽

Bone Mineral ◽

Mineral Content ◽

Intact Male ◽

Age Related ◽

F344 Rats ◽

Male F344 Rats ◽

Age Related Changes

Download Full-text

Age-related changes in the haematology of female F344 rats

Laboratory Animals ◽

10.1258/002367789780746006 ◽

1989 ◽

Vol 23 (4) ◽

pp. 295-301 ◽

Cited By ~ 17

Author(s):

J. A. Turton ◽

C. M. Hawkey ◽

M. G. Hart ◽

J. Gwynne ◽

R. M. Hicks

Keyword(s):

Cell Types ◽

Age Group ◽

Cell Type ◽

Rat Strains ◽

Lymphoid Leukaemia ◽

Adult Level ◽

Age Related ◽

Haematological Changes ◽

F344 Rats ◽

Age Related Changes

As little comprehensive baseline data are available on age-related haematological changes in genetically-defined rat strains, the haematology of female F344 rats is described in animals sampled at 2, 4, 8, 20, 66 and 121 weeks of age. Values for Hb, RBC and PCV increased from 2 weeks of age to reach adult levels at 8 weeks, whereas MCV, MCH and reticulocyte counts were high initially but decreased to reach the adult range at 8 weeks. Between 66 and 121 weeks, reticulocyte counts were significantly increased and values for MCHC significantly decreased. Lymphocytes were the predominant white cell type in each age group. The absolute numbers of neutrophils and lymphocytes showed slight variations between 2 and 66 weeks and both cell types increased significantly between 66 and121 weeks. Platelet counts showed no overall age-related trends. Fibrinogen values increased from 2 weeks of age to reach the adult level at 8 weeks. One animal of the 14 sampled at 121 weeks showed changes in the blood, liver and spleen consistent with a diagnosis of lymphoid leukaemia.

Download Full-text