Age-related changes in the haematology of female F344 rats

1989 ◽  
Vol 23 (4) ◽  
pp. 295-301 ◽  
Author(s):  
J. A. Turton ◽  
C. M. Hawkey ◽  
M. G. Hart ◽  
J. Gwynne ◽  
R. M. Hicks
Keyword(s):  
Cell Types ◽  
Age Group ◽  
Cell Type ◽  
Rat Strains ◽  
Lymphoid Leukaemia ◽  
Adult Level ◽  
Age Related ◽  
Haematological Changes ◽  
F344 Rats ◽  
Age Related Changes

As little comprehensive baseline data are available on age-related haematological changes in genetically-defined rat strains, the haematology of female F344 rats is described in animals sampled at 2, 4, 8, 20, 66 and 121 weeks of age. Values for Hb, RBC and PCV increased from 2 weeks of age to reach adult levels at 8 weeks, whereas MCV, MCH and reticulocyte counts were high initially but decreased to reach the adult range at 8 weeks. Between 66 and 121 weeks, reticulocyte counts were significantly increased and values for MCHC significantly decreased. Lymphocytes were the predominant white cell type in each age group. The absolute numbers of neutrophils and lymphocytes showed slight variations between 2 and 66 weeks and both cell types increased significantly between 66 and121 weeks. Platelet counts showed no overall age-related trends. Fibrinogen values increased from 2 weeks of age to reach the adult level at 8 weeks. One animal of the 14 sampled at 121 weeks showed changes in the blood, liver and spleen consistent with a diagnosis of lymphoid leukaemia.

Nomifensine reveals age-related changes in K + -evoked striatal DA overflow in F344 rats

2001 ◽  
Vol 22 (3) ◽  
pp. 495-502 ◽  
Author(s):  
John A. Stanford ◽  
Theresa D. Currier ◽  
Matthew S. Purdom ◽  
Greg A. Gerhardt
Keyword(s):  
Age Related ◽  
F344 Rats ◽  
Age Related Changes

Effects of Aging and TGF-Beta 3 on Chondrocyte and Mesenchymal Stem Cell Matrix Formation

2010 ◽  
Author(s):  
Isaac E. Erickson ◽  
Steven C. van Veen ◽  
Swarnali Sengupta ◽  
Sydney R. Kestle ◽  
Jason A. Burdick ◽  
...  
Keyword(s):  
Stem Cells ◽  
Articular Chondrocytes ◽  
Cell Types ◽  
Matrix Proteins ◽  
Cell Type ◽  
Cell Matrix ◽  
Age Related ◽  
Cartilage Restoration ◽  
Effects Of Aging

Articular cartilage pathology is common in the aged population. Numerous studies have shown that aged chondrocytes (CHs) are inferior to juvenile CHs in their ability to proliferate and produce cartilage-specific extracellular matrix proteins, potentially limiting their use in tissue engineering applications for cartilage restoration [1,2]. Mesenchymal stem cells (MSCs) are an alternative cell type that can be expanded in vitro while maintaining their ability to differentiate into cell types comparable to articular chondrocytes. However, organismal aging also influences human MSC proliferation [3,4] and multi-potential differentiation [5], though for chondrogenesis these findings are mixed, with some suggesting that aged progenitor cells retain their chondrogenic capacity [6]. The objective of this study was to assess age related differences in donor-matched CH and MSC potential for chondrogenic repair. In addition, the effects of the chondrogenic growth factor TGF-β3 on CHs and MSCs were evaluated.

scholarly journals Microarray analysis of aging-associated immune system alterations in the rostral ventrolateral medulla of F344 rats

2017 ◽  
Vol 49 (8) ◽  
pp. 400-415 ◽  
Author(s):  
Sivasai Balivada ◽  
Chanran K. Ganta ◽  
Yongqing Zhang ◽  
Hitesh N. Pawar ◽  
Richard J. Ortiz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune System ◽  
Neural Substrates ◽  
Rostral Ventrolateral Medulla ◽  
Ventrolateral Medulla ◽  
Middle Aged ◽  
Transcriptomic Profile ◽  
Age Related ◽  
F344 Rats ◽  
Age Related Changes

The rostral ventrolateral medulla (RVLM) is an area of the brain stem that contains diverse neural substrates that are involved in systems critical for physiological function. There is evidence that aging affects some neural substrates within the RVLM, although age-related changes in RVLM molecular mechanisms are not well established. The goal of the present study was to characterize the transcriptomic profile of the aging RVLM and to test the hypothesis that aging is associated with altered gene expression in the RVLM, with an emphasis on immune system associated gene transcripts. RVLM tissue punches from young, middle-aged, and aged F344 rats were analyzed with Agilent’s whole rat genome microarray. The RVLM gene expression profile varied with age, and an association between chronological age and specific RVLM gene expression patterns was observed [ P < 0.05, false discovery rate (FDR) < 0.3]. Functional analysis of RVLM microarray data via gene ontology profiling and pathway analysis identified upregulation of genes associated with immune- and stress-related responses and downregulation of genes associated with lipid biosynthesis and neurotransmission in aged compared with middle-aged and young rats. Differentially expressed genes associated with the complement system and microglial cells were further validated by quantitative PCR with separate RVLM samples ( P < 0.05, FDR < 0.1). The present results have identified age-related changes in the transcriptomic profile of the RVLM, modifications that may provide the molecular backdrop for understanding age-dependent changes in physiological regulation.

scholarly journals Changes in Cellular Crosstalk between Skeletal Muscle Myoblasts and Bone Osteoblasts with Aging

2021 ◽  
Author(s):  
Jonathan A. Doering ◽  
Carly E. Britt ◽  
Gregory S. Sawicki ◽  
Jacqueline H. Cole
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
Cell Types ◽  
Cell Type ◽  
Bone Mesenchymal Stem Cells ◽  
Tissue Mass ◽  
Age Related ◽  
Proliferation And Differentiation ◽  
Potent Growth ◽  
Musculoskeletal Function

AbstractMusculoskeletal function declines with aging, resulting in an increased incidence of trips and falls. Both bone and muscle experience age-related losses in tissue mass that alter their mechanical interactions in a well characterized manner, but changes in the biochemical interactions between bone and muscle with aging are not well understood. Of note, insulin-like growth factor 1 (IGF-1), a potent growth factor for bone and muscle, can be negatively altered with aging and may help explain losses in these tissues. We recently developed a co-culture system for simultaneous growth of bone mesenchymal stem cells (MSCs) and muscle satellite cells (SCs) to investigate the biochemical crosstalk between the two cell types. Here, we utilized an aging rat model to study cellular changes between young and old rat MSCs and SCs, in particular whether 1) young MSCs and SCs have increased proliferation and differentiation compared to old MSCs and SCs; 2) young cells have increased IGF-1 and collagen expression as a measure of crosstalk compared to old cells; and 3) young cells can mitigate the aging phenotype of old cells in co-culture. Rat MSCs and SCs were either mono- or co-cultured in Transwell® plates, grown to confluence, and allowed to differentiate for 14 days. Across the 14 days, cell proliferation was measured, with differentiation and crosstalk measurements evaluated at 14 days. The results suggest that in both young and old, proliferation is greater in mono-cultures compared to co-cultures, yet age and cell type did not have a significant effect. Differentiation did not differ between young and old cells, yet MSCs and SCs demonstrated the greatest amount of differentiation in co-culture. Finally, age, cell type, and culture type did not have a significant effect on collagen or IGF-1 expression. These results suggest co-culture may have a controlling effect, with the two cell types acting together to promote differentiation more than in mono-cultures, yet this response was not altered by age. In general, results for old cells had higher variability, suggesting a wider variety in the aging phenotypes demonstrated in these animals. This study was the first to use this rat aging model to investigate changes between bone and skeletal muscle cells, however further investigations are required to determine what signaling changes occur in response to age. Determining these signaling changes could lead to new targets for mitigating the progression of aging.

scholarly journals Computed tomographic assessment of equine maxillary cheek teeth anatomical relationships, and paranasal sinus volumes

2017 ◽  
Vol 181 (17) ◽  
pp. 452-452 ◽  
Author(s):  
Tiziana Liuti ◽  
Richard Reardon ◽  
Paddy M Dixon
Keyword(s):  
Maxillary Sinus ◽  
Age Group ◽  
Medial Aspect ◽  
Computed Tomographic ◽  
Age Related ◽  
Infraorbital Canal ◽  
The Mean ◽  
The Individual ◽  
Gross Examination ◽  
Age Related Changes

Disorders affecting the equine maxillary cheek teeth and paranasal sinuses are relatively common, but limited objective information is available on the dimensions and relationships of these structures in horses of different ages. The aims of this study were to assess age-related changes in the positioning and anatomical relationships of the individual maxillary cheek teeth with the infraorbital canal and maxillary septum and the volumes of the individual sinus compartments. CT and gross examination were performed on 60 normal equine cadaver heads that were aged by their dentition. The intrasinus position of cheek teeth, length of reserve crowns, relationship to the infraorbital canal and measurements of rostral drift and sinus compartment volumes were assessed from CT images. The findings included that Triadan 10 alveoli lay fully or partially in the rostral maxillary sinus (RMS) in 60% of cases. The infraorbital canal lay directly on the medial aspect of the alveolar apex in younger horses. The Triadan 11’sclinical crowns and apices drifted a mean of 2.48 and 2.83 cm more rostral to the orbit, respectively, in the >15 years old vs the <6 years old age group. The mean volumes of sinus compartments ranged from 175 cm3for the caudal maxillary sinus (CMS) to 4 cm3for the ethmoidal sinus (ES). This information should be of value in the diagnosis and treatment of equine dental and sinus disorders and as reference values for further studies.

scholarly journals Conductive Hearing Loss with Age—A Histologic and Audiometric Evaluation

2021 ◽  
Vol 10 (11) ◽  
pp. 2341
Author(s):  
Ivo Dobrev ◽  
Daniel Dillinger ◽  
Letizia Meier ◽  
Dorothe Veraguth ◽  
Flurin Pfiffner ◽  
...  
Keyword(s):  
Hearing Loss ◽  
Conductive Hearing Loss ◽  
Age Groups ◽  
Joint Space ◽  
Age Group ◽  
Interindividual Variations ◽  
Age Related ◽  
Average Change ◽  
Conductive Hearing ◽  
Age Related Changes

A retrospective analysis to quantify age-related changes of the incudo-malleolar joint (IMJ) and incudo-stapedial joint (ISJ), and to analyse changes in the air-bone gap (ABG) with age, was performed. Defined histologic parameters of 153 IMJ and 106 ISJ from subjects aged from birth to 70 years were correlated to age. Additionally, audiograms of 1760 ears of 974 other subjects aged 20 to 80 years were retrospectively analysed and the ABG was correlated to age. The joint space (age group from 0 to 10 compared to 61 to 70 years) became significantly wider with age (IMJ: from a mean of 44 µm to 100 µm, p < 0.001; ISJ: from a mean of 28 µm to 69 µm, p < 0.009. The thickness of cartilage of the incus decreased in the first 20 years of life (IMJ, from a mean of 88 µm to 65 µm, p < 0.01; ISJ: from a mean of 44 µm to 35 µm, p < 0.01). The ABGs of younger ears (20–40 years) was significantly larger at 500 Hz compared to older ears (60–80 years) by 2–4 dB, while it was significantly smaller by 3–5 dB at 4000 Hz (p < 0.0017). Interindividual variations in all age groups were large for both analyses. The increased joint spaces could potentially reduce the stiffness in the joints and explain the increase in ABG at 4000 Hz and the drop at 500 Hz. While the average change is small and of minimal clinical relevance, a larger increase of ABG with age is seen in some subjects.

scholarly journals Hallmarks of Aging in Macrophages: Consequences to Skin Inflammaging

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1323
Author(s):  
Gabriela Rapozo Guimarães ◽  
Palloma Porto Almeida ◽  
Leandro de Oliveira Santos ◽  
Leane Perim Rodrigues ◽  
Juliana Lott de Carvalho ◽  
...  
Keyword(s):  
Adaptive Immunity ◽  
Cellular Proliferation ◽  
Immune Cell ◽  
Disease Onset ◽  
Cell Types ◽  
Therapeutic Interventions ◽  
Cell Type ◽  
Innate And Adaptive Immunity ◽  
Age Related ◽  
Effects Of Aging

The skin is our largest organ and the outermost protective barrier. Its aging reflects both intrinsic and extrinsic processes resulting from the constant insults it is exposed to. Aging in the skin is accompanied by specific epigenetic modifications, accumulation of senescent cells, reduced cellular proliferation/tissue renewal, altered extracellular matrix, and a proinflammatory environment favoring undesirable conditions, including disease onset. Macrophages (Mφ) are the most abundant immune cell type in the skin and comprise a group of heterogeneous and plastic cells that are key for skin homeostasis and host defense. However, they have also been implicated in orchestrating chronic inflammation during aging. Since Mφ are related to innate and adaptive immunity, it is possible that age-modified skin Mφ promote adaptive immunity exacerbation and exhaustion, favoring the emergence of proinflammatory pathologies, such as skin cancer. In this review, we will highlight recent findings pertaining to the effects of aging hallmarks over Mφ, supporting the recognition of such cell types as a driving force in skin inflammaging and age-related diseases. We will also present recent research targeting Mφ as potential therapeutic interventions in inflammatory skin disorders and cancer.

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