scholarly journals Microarray analysis of aging-associated immune system alterations in the rostral ventrolateral medulla of F344 rats

2017 ◽  
Vol 49 (8) ◽  
pp. 400-415 ◽  
Author(s):  
Sivasai Balivada ◽  
Chanran K. Ganta ◽  
Yongqing Zhang ◽  
Hitesh N. Pawar ◽  
Richard J. Ortiz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune System ◽  
Neural Substrates ◽  
Rostral Ventrolateral Medulla ◽  
Ventrolateral Medulla ◽  
Middle Aged ◽  
Transcriptomic Profile ◽  
Age Related ◽  
F344 Rats ◽  
Age Related Changes

The rostral ventrolateral medulla (RVLM) is an area of the brain stem that contains diverse neural substrates that are involved in systems critical for physiological function. There is evidence that aging affects some neural substrates within the RVLM, although age-related changes in RVLM molecular mechanisms are not well established. The goal of the present study was to characterize the transcriptomic profile of the aging RVLM and to test the hypothesis that aging is associated with altered gene expression in the RVLM, with an emphasis on immune system associated gene transcripts. RVLM tissue punches from young, middle-aged, and aged F344 rats were analyzed with Agilent’s whole rat genome microarray. The RVLM gene expression profile varied with age, and an association between chronological age and specific RVLM gene expression patterns was observed [ P < 0.05, false discovery rate (FDR) < 0.3]. Functional analysis of RVLM microarray data via gene ontology profiling and pathway analysis identified upregulation of genes associated with immune- and stress-related responses and downregulation of genes associated with lipid biosynthesis and neurotransmission in aged compared with middle-aged and young rats. Differentially expressed genes associated with the complement system and microglial cells were further validated by quantitative PCR with separate RVLM samples ( P < 0.05, FDR < 0.1). The present results have identified age-related changes in the transcriptomic profile of the RVLM, modifications that may provide the molecular backdrop for understanding age-dependent changes in physiological regulation.

scholarly journals Inflamm-Aging Is Associated with Lower Plasma PTX3 Concentrations and an Impaired Capacity of PBMCs to Express hTERT following LPS Stimulation

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Aaron L. Slusher ◽  
Tiffany M. Zúñiga ◽  
Edmund O. Acevedo
Keyword(s):  
Gene Expression ◽  
Young Adults ◽  
Telomere Length ◽  
Immune Cell ◽  
Ex Vivo ◽  
Middle Aged ◽  
Human Telomerase Reverse Transcriptase ◽  
Negatively Associated ◽  
Htert Gene ◽  
Age Related

Age-related elevations in proinflammatory cytokines, known as inflamm-aging, are associated with shorter immune cell telomere lengths. Purpose. This study examined the relationship of plasma PTX3 concentrations, a biomarker of appropriate immune function, with telomere length in 15 middle-aged (40-64 years) and 15 young adults (20-31 years). In addition, PBMCs were isolated from middle-aged and young adults to examine their capacity to express a key mechanistic component of telomere length maintenance, human telomerase reverse transcriptase (hTERT), following ex vivo cellular stimulation. Methods. Plasma PTX3 and inflammatory cytokines (i.e., IL-6, IL-10, TGF-β, and TNF-α), PBMC telomere lengths, and PBMC hTERT gene expression and inflammatory protein secretion following exposure to LPS, PTX3, and PTX3+LPS were measured. Results. Aging was accompanied by the accumulation of centrally located visceral adipose tissue, without changes in body weight and BMI, and alterations in the systemic inflammatory milieu (decreased plasma PTX3 and TGF-β; increased TNF-α (p≤0.050)). In addition, shorter telomere lengths in middle-aged compared to young adults (p=0.011) were negatively associated with age, body fat percentages, and plasma TNF-α (r=−0.404, p=0.027; r=−0.427, p=0.019; and r=−0.323, p=0.041, respectively). Finally, the capacity of PBMCs to increase hTERT gene expression following ex vivo stimulation was impaired in middle-aged compared to young adults (p=0.033) and negatively associated with telomere lengths (r=0.353, p=0.028). Conclusions. Proinflammation and the impaired hTERT gene expression capacity of PBMCs may contribute to age-related telomere attrition and disease.

Nomifensine reveals age-related changes in K + -evoked striatal DA overflow in F344 rats

2001 ◽  
Vol 22 (3) ◽  
pp. 495-502 ◽  
Author(s):  
John A. Stanford ◽  
Theresa D. Currier ◽  
Matthew S. Purdom ◽  
Greg A. Gerhardt
Keyword(s):  
Age Related ◽  
F344 Rats ◽  
Age Related Changes

Perceptions of Sex Roles of Young and Middle-Aged Women and Men

1982 ◽  
Vol 55 (2) ◽  
pp. 559-562
Author(s):  
Claire Etaugh ◽  
Sharon Weber
Keyword(s):  
College Students ◽  
Sex Roles ◽  
Sex Role ◽  
Middle Aged ◽  
Age Related ◽  
Aged Woman ◽  
Male College ◽  
Middle Aged Woman ◽  
Age Related Changes ◽  
Female Subjects

48 female and 48 male college students used the Bern Sex-role Inventory to describe either a young or middle-aged woman or man. Female subjects perceived that women become increasingly feminine and less androgynous with age. No age-related changes were perceived in men's sex-role behaviors.

Age-related changes in pancreatic islet cell gene expression

Metabolism ◽  
1995 ◽  
Vol 44 (3) ◽  
pp. 320-324 ◽  
Author(s):  
Stephen J. Giddings ◽  
Lynn R. Carnaghi ◽  
Arshag D. Mooradian
Keyword(s):  
Gene Expression ◽  
Pancreatic Islet ◽  
Islet Cell ◽  
Pancreatic Islet Cell ◽  
Age Related ◽  
Cell Gene Expression ◽  
Cell Gene ◽  
Age Related Changes

scholarly journals The Impact of Long‐Term Physical Activity on Age‐Related Changes in Protein and Gene Expression

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Ian R Lanza ◽  
Daniel K Short ◽  
Kevin R Short ◽  
Yan W Asmann ◽  
Sreekumar Raghavakaimal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Physical Activity ◽  
Age Related ◽  
The Impact ◽  
Age Related Changes

Age-Related Changes in the Murine Immune System

2021 ◽  
pp. 195-204
Author(s):  
Janko Nikolich-Zugich ◽  
Nico A. Contreras ◽  
Christopher P. Coplen ◽  
Ilija Jeftic
Keyword(s):  
Immune System ◽  
Age Related ◽  
Age Related Changes

scholarly journals Age-related DNA hydroxymethylation is enriched for gene expression and immune system processes in human peripheral blood

Epigenetics ◽  
2019 ◽  
Vol 15 (3) ◽  
pp. 294-306 ◽  
Author(s):  
Nicholas D. Johnson ◽  
Luoxiu Huang ◽  
Ronghua Li ◽  
Yun Li ◽  
Yuchen Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune System ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Dna Hydroxymethylation ◽  
Age Related

A model to assess age-related changes in two-point discrimination of plantar skin

1999 ◽  
Vol 89 (8) ◽  
pp. 383-391 ◽  
Author(s):  
W Lynch ◽  
J Mooney
Keyword(s):  
Older Adults ◽  
Statistical Analysis ◽  
Linear Relationship ◽  
Middle Aged ◽  
Discrimination Accuracy ◽  
Discrimination Ability ◽  
Fixed Distance ◽  
Age Related ◽  
Older Subjects ◽  
Age Related Changes

A device was constructed to assess two-point discrimination in the plantar skin of younger, middle-aged, and older adults. The device consisted of paired filaments, each delivering a tip force of 4.56 log units (equivalent to 3.63 g), at a fixed distance apart. Statistical analysis showed significant differences in two-point discrimination accuracy between these groups of subjects. Younger subjects showed greater accuracy of plantar two-point discrimination than either middle-aged or older subjects, with an apparent inverse linear relationship between subject age and plantar two-point discrimination ability. A model to assess age-related changes in two-point discrimination is presented.

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