The fine structure of pea root nodules. 1. Vacuolar changes after endocytotic host cell infection by Rhizobium leguminosarum

Pisum sativum L. (pea) root nodule cells undergo many cellular changes in response to infection by Rhizobium leguminosarum bv. viciae. These include cell growth, organelle reorganization, and changes relating to the increase in the number of bacteria within the cell. The objective of this study was to characterize microtubule organization during nodule cell development. The organization of microtubules was examined in developing pea root nodules using fluorescence and electron microscopy techniques. Immunolabelling of microtubules in meristematic cells showed diffuse fluorescence in the cell cortex and adjacent to the nuclear envelope. Recently infected cells contained randomly oriented cortical microtubules and cytoplasmic microtubules that were fragmented with diffuse fluorescence. Infected cells contained an extensive network of long, randomly arranged cortical microtubules with some parallel bundles. Cytoplasmic microtubules in single optical sections of infected cells appeared as short undulating filaments; however, overlapping images from a Z-series of an infected cell showed that the microtubules are long and wavy, and generally radiate inward from the cell cortex.Key words: nodule, microtubules, Rhizobium, pea, symbiosis.

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Isolation of monoclonal antibodies reacting with peribacteriod membranes and other components of pea root nodules containing Rhizobium leguminosarum

Planta ◽

10.1007/bf00403006 ◽

1988 ◽

Vol 173 (2) ◽

pp. 149-160 ◽

Cited By ~ 83

Author(s):

D. J. Bradley ◽

E. A. Wood ◽

A. P. Larkins ◽

G. Galfre ◽

G. W. Butcher ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Rhizobium Leguminosarum ◽

Root Nodules ◽

Pea Root

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The Use of Monoclonal Antibodies to Investigate Plant-Microbe Interactions in Pea Root Nodules Containing Rhizobium Leguminosarum

Cell to Cell Signals in Plant, Animal and Microbial Symbiosis ◽

10.1007/978-3-642-73154-9_26 ◽

1988 ◽

pp. 373-383 ◽

Cited By ~ 1

Author(s):

N. J. Brewin ◽

D. J. Bradley ◽

E. A. Wood ◽

E. L. Kannenberg ◽

K. A. VandenBosch ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Rhizobium Leguminosarum ◽

Root Nodules ◽

Pea Root ◽

Microbe Interactions

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Localizatlon of expansin-like protein in apoplast of pea (Pisum sativum L.) root nodules during interaction with Rhizobium leguminosarum bv. viciae

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2007.002 ◽

2011 ◽

Vol 76 (1) ◽

pp. 17-26 ◽

Cited By ~ 6

Author(s):

Marzena Sujkowska ◽

Wojciech Borucki ◽

Władysław Golinowski

Keyword(s):

Cell Wall ◽

Plant Cell ◽

Rhizobium Leguminosarum ◽

Plant Cell Wall ◽

Root Nodules ◽

Infection Thread ◽

Nodule Development ◽

Cell Enlargement ◽

Pea Root ◽

Pea Roots

During nodule development on pea roots, apoplast undergoes changes in activity of plant cell wall proteins such as expansins (EXPs). Because the accumulation of EXP protein has been correlated with the growth of various plant organs, we investigated using Western Blot and immunolocalization studies with antibody against PsEXP1, whether this protein was accumulated in the expanding cells of nodule. Immunoblot results indicated the presence of a 30-kDa band specific for pea root nodules. The EXP proteins content rose during growth of pea root nodules. Expansin(s) protein was localized in nodule apoplast as well as in the infection thread walls. The enhanced amount of expansin-like proteins in meristematic part of nodule, root and shoot was shown. The localization of this protein in the meristematic cell walls can be related to the loosening of plant cell wall before cell enlargement. Both, plant cell enlargement and infection thread growth require activity of expansin(s). Possible involvement of EXPs in the process of pea root nodule development is also discussed.

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The Infection of Cells by Bullet-Shaped Viruses

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100063779 ◽

1969 ◽

Vol 27 ◽

pp. 372-373

Author(s):

Frederick A. Murphy ◽

Alyne K. Harrison ◽

Sylvia G. Whitfield

Keyword(s):

Electron Microscopy ◽

Physical Properties ◽

Host Cell ◽

Thin Section ◽

Cultured Cells ◽

Cell Infection ◽

Thin Section Electron Microscopy ◽

Section Electron ◽

Section Electron Microscopy

The bullet-shaped viruses are currently classified together on the basis of similarities in virion morphology and physical properties. Biologically and ecologically the member viruses are extremely diverse. In searching for further bases for making comparisons of these agents, the nature of host cell infection, both in vivo and in cultured cells, has been explored by thin-section electron microscopy.

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COVID 19 – Eye Issues

Medical & Clinical Research ◽

10.33140/mcr.05.005 ◽

2020 ◽

Vol 5 (Special) ◽

Keyword(s):

Host Cell ◽

Target Cell ◽

Cell Receptor ◽

Human Tissues ◽

Type Ii ◽

Cell Infection ◽

Host Cell Receptor ◽

Infected Cells ◽

Cellular Entry

The coronavirus illness (COVID-19) is caused by a new recombinant SARS-CoV (SARS-CoV) virus (SARS-CoV-2). Target cell infection by SARS-CoV is mediated by the prickly protein of the coronavirus and host cell receptor, enzyme 2 converting angiotensin (ACE2) [3]. Similarly, a recent study suggests that cellular entry by SARS-CoV-2 is dependent on both ACE2 as well as type II transmembrane axial protease (TMPRSS2) [4]. This means that detection of ACE2 and PRSS2 expression in human tissues can predict potential infected cells and their respective effects in COVID-19 patients [1].

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