[63] Fractionation of mammalian blood cells by partition in two-polymer aqueous phases

The interfacial tension method has been applied to the study of the surface composition of mammalian blood cells and to certain other particles. Unsensitized erythrocytes and stromata possess only a small margin of stability in the interface and pass readily into the oil phase. Specifically sensitized erythrocytes and stromata possess much greater stability in the interface and pass into the oil only with considerable mechanical aid; characteristic deformations of the erythrocyte surface or the interface or both often result. With special immune sera prepared by Landsteiner and van der Scheer the quantitative relations are such as to indicate that the increased polarity of the sensitized erythrocyte surface is due to combination of the red cell surface lipoids with hemolytic sensitizer. These results are corroborative of the conclusion of Landsteiner and van der Scheer that erythrocytes contain specific lipoid-soluble antigens. The tentative conclusion is reached that with these anti horse-erythrocyte sera at least the agglutinins combine predominantly with the protein of the red cell surfaces. Fresh human leucocytes are spread and disintegrated by the interfacial stresses. After heat injury over the condenser with substage lamp the leucocytes typically do not enter the boundary surface. They are pushed before the advancing interface and, if their further advance is obstructed, bend the interface backward to form peninsulas and vacuoles. This change after heating is in the opposite sense to that to be expected from denaturation of the proteins of the protoplasm. Fresh oxalated rabbit platelets pass very easily into the oils. After heating over the substage lamp these elements also become less oil-miscible. The interfacial tension relations of blood cells, bacteria, and several cell products are tabulated.

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Microtubules and Filaments in Blood Platelets

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100070321 ◽

1978 ◽

Vol 36 (3) ◽

pp. 597-605

Author(s):

O. Behnke ◽

J. Tranum-Jensen

Keyword(s):

Bone Marrow ◽

Blood Cells ◽

Blood Platelets ◽

Blood Platelet ◽

Main Function ◽

Mammalian Blood ◽

Recent Monograph ◽

Vital Microscopy ◽

Rigid Skeleton

Within the last decade the mammalian blood platelet has enjoyed a rapidly increasing popularity as a research object. It is widely appreciated that platelets are multifunctional, and that they play a role in a variety of physiological and pathological processes besides their main function: the formation and consolidation of a haemostatic plug (for a recent monograph, and for references, see Gordon, 1976). The platelet is an anucleate piece of cytoplasm, pinched off from megakaryocytes (MK) in the bone marrow or in the circulation (Tinggaard Petersen, 1974). Vital microscopy has shown that in the circulation the platelet is disc-shaped, that it normally does not stick to endothelium or to other blood cells, and that it is the least deformable cell in blood - the latter observation does suggest the presence of a rigid skeleton in the platelet cytoplasm.

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AN INVESTIGATION BY ELECTRON MICROSCOPY OF THE NUCLEOSIDE PHOSPHATASE ACTIVITY OF AMPHIBIAN AND MAMMALIAN ERYTHROCYTES

The Journal of Cell Biology ◽

10.1083/jcb.26.1.209 ◽

1965 ◽

Vol 26 (1) ◽

pp. 209-217 ◽

Cited By ~ 5

Author(s):

J. Tooze

Keyword(s):

Electron Microscopy ◽

Blood Cells ◽

Epoxy Resins ◽

Osmium Tetroxide ◽

Plasma Membranes ◽

Isotonic Saline ◽

Rana Esculenta ◽

Triturus Cristatus ◽

Mammalian Blood ◽

The Media

Amphibian and mammalian blood was washed in isotonic saline, fixed in glutaraldehyde, and then stained in the ATPase medium of Wachstein and Meisel. The blood cells were subsequently postfixed in osmium tetroxide, embedded in epoxy resins, and studied by electron microscopy. The plasma membranes of amphibian erythrocytes, from the newt Triturus cristatus and the frog Rana esculenta, were stained after incubation in media containing ATP or ADP as substrates, but were unstained after incubation in media containing AMP or sodium ß-glycerophosphate. The addition of 0.001 M ouabain to ATP-containing media did not inhibit the staining of the plasma membranes, but the omission of Mg++ ions from the medium inhibited staining. The plasma membranes of rat and rabbit erythrocytes were never stained after incubation in any of the media used.

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Nanoscale Dielectrophoretic Spectroscopy of Individual Immobilized Mammalian Blood Cells

Biophysical Journal ◽

10.1529/biophysj.106.082412 ◽

2006 ◽

Vol 91 (7) ◽

pp. 2678-2686 ◽

Cited By ~ 13

Author(s):

Brian P. Lynch ◽

Al.M. Hilton ◽

Garth J. Simpson

Keyword(s):

Blood Cells ◽

Mammalian Blood

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Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083266 ◽

1978 ◽

Vol 36 (2) ◽

pp. 480-481

Author(s):

Kosuke Ueda ◽

Hiroto Washida ◽

Nakazo Watari

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Renal Cortex ◽

Uranyl Acetate ◽

Osmic Acid ◽

Renal Lithiasis ◽

Electron Microscopic ◽

Hemoglobin C ◽

First Case ◽

Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

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Ultrastructural changes in murine lymphoma cells exposed to ultraviolet-A radiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010008482x ◽

1991 ◽

Vol 49 ◽

pp. 104-105

Author(s):

Delma P. Thomas ◽

Dianne E. Godar

Keyword(s):

Medical Devices ◽

Blood Cells ◽

White Blood Cells ◽

Consumer Products ◽

Ultrastructural Changes ◽

Ultraviolet A ◽

Uv Spectrum ◽

Lymphoma Cells ◽

Murine Lymphoma ◽

Transmission Electron

Ultraviolet radiation (UVR) from all three waveband regions of the UV spectrum, UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm), can be emitted by some medical devices and consumer products. Sunlamps can expose the blood to a considerable amount of UVR, particularly UVA and/or UVB. The percent transmission of each waveband through the epidermis to the dermis, which contains blood, increases in the order of increasing wavelength: UVC (10%) < UVB (20%) < UVA (30%). To investigate the effects of UVR on white blood cells, we chose transmission electron microscopy to examine the ultrastructure changes in L5178Y-R murine lymphoma cells.

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Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072083 ◽

1973 ◽

Vol 31 ◽

pp. 328-329

Author(s):

John A. Trotter

Keyword(s):

Red Blood Cells ◽

Analytical Method ◽

Blood Cells ◽

Guinea Pigs ◽

Specific Protein ◽

Visual Examination ◽

Hemoglobin Synthesis ◽

Peroxidatic Activity ◽

Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

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