9.8 Influence of pentamidine on human neutrophilic granulocyte function

ABSTRACTPrevious studies have suggested that neonates rely heavily on innate immunity for their antimicrobial response to bacterial infections. However, the innate immune response by neonates to bacterial infection remains poorly characterized. Here, we show that in a murine model of neonatal polymicrobial sepsis, CXC ligand 10 (CXCL10) concentrations increase in the blood and peritoneum concordant with the peritoneal recruitment of granulocytes and macrophages. Additionally, CXC receptor 3 (CXCR3) expression on elicited peritoneal macrophages and granulocytes increases following sepsis. Blockade of CXCL10 worsens not only recruitment and phagocytic function of peritoneal granulocytes and macrophages but also survival. Deletion of CXCR3 also significantly increases mortality to a septic challenge. Finally, we demonstrate that the protective adjuvant effect of pretreatment with a Toll-like receptor 4 agonist to neonatal sepsis is dependent on an endogenous CXCL10 response and that pretreatment of neonates with CXCL10 can also significantly improve macrophage and granulocyte function and modestly improve outcome to polymicrobial sepsis. Together, these data suggest a critical role for CXCL10 signaling during neonatal sepsis.

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BENZODIAZEPINES AND THEIR SOLVENTS INFLUENCE NEUTROPHIL GRANULOCYTE FUNCTION

British Journal of Anaesthesia ◽

10.1093/bja/70.3.317 ◽

1993 ◽

Vol 70 (3) ◽

pp. 317-321 ◽

Cited By ~ 22

Author(s):

M. WEISS ◽

N. MIROW ◽

A. BIRKHAHN ◽

M. SCHNEIDER ◽

P. WERNET

Keyword(s):

Neutrophil Granulocyte ◽

Granulocyte Function

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Granulocyte Function in Recipients of Renal Transplants from Live Related Donors and from Cadavers

The Journal of Infectious Diseases ◽

10.1093/infdis/142.6.876 ◽

1980 ◽

Vol 142 (6) ◽

pp. 876-883 ◽

Cited By ~ 1

Author(s):

N. Golshan ◽

C. F. Barker ◽

R. R. MacGregor

Keyword(s):

Renal Transplants ◽

Granulocyte Function

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NORMAL GRANULOCYTE INFUSION THERAPY FOR ASPERGILLOSIS IN CHRONIC GRANULOMATOUS DISEASE

PEDIATRICS ◽

10.1542/peds.51.2.230 ◽

1973 ◽

Vol 51 (2) ◽

pp. 230-233

Author(s):

Andrew A. Raubitschek ◽

Alan S. Levin ◽

Daniel P. Stites ◽

Edward B. Shaw ◽

H. Hugh Fudenberg

Keyword(s):

Adverse Effects ◽

Chronic Granulomatous Disease ◽

Blood Cells ◽

White Blood Cells ◽

Infusion Therapy ◽

Granulomatous Disease ◽

Transient Improvement ◽

Life Threatening ◽

Granulocyte Function

An 8-year-old boy with chronic granulomatous disease (CGD) was admitted in moribund condition with aspergillus pneumonia. Because of the gravity of the situation, normal granulocyte infusions were used as adjuncts to the more conventional antimicrobial therapy. White blood cells, derived from a total of 58 units of whole blood obtained by leukophoresis of the father, were given in two separate doses. The first dose, totaling 2.8 x 1010 granulocytes, was coincident with significant improvement, and the second, totaling 3.0 x 1010 granulocytes, was coincident with the onset of clinical improvement and interim recovery. Transient improvement in in vitro granulocyte function was noted in cells taken from the patient's blood immediately after infusion. No adverse effects of the infusions were noted in either the patient or the donor. Although it is impossible to divorce the therapeutic effect of the granulocyte infusions from the more conventional therapy, we conclude that normal granulocyte infusions can be considered a valid adjunct in children with CGD who are suffering from a life-threatening infection.

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Characterization of the Priming Effect by Pituitary Canine Growth Hormone on Canine Polymorphonuclear Neutrophil Granulocyte Function

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.7.2.226-232.2000 ◽

2000 ◽

Vol 7 (2) ◽

pp. 226-232 ◽

Cited By ~ 1

Author(s):

Thomas K. Petersen ◽

C. Wayne Smith ◽

Asger L. Jensen

Keyword(s):

Growth Hormone ◽

Priming Effect ◽

Shape Change ◽

Transendothelial Migration ◽

Lower Surface ◽

Polymorphonuclear Neutrophil ◽

Neutrophil Granulocytes ◽

Neutrophil Granulocyte ◽

Microtiter Plates ◽

Granulocyte Function

ABSTRACT In this report, we demonstrate that canine growth hormone (cGH) is capable of priming canine polymorphonuclear neutrophil granulocytes (PMN) in a manner resembling that of human PMN. The cGH influences important functions that are involved in the process of recruitment of PMN, i.e., shape change, chemotaxis, CD11b/CD18 expression, adhesion, and subsequent transendothelial migration. Also, intracellular O2 − production was evaluated. We investigated the priming effect by incubating PMN with purified pituitary cGH at various concentrations (10 to 800 μg/liter). The capacity for shape change was significantly (P < 0.05) enhanced, whereas the chemotactic response under agarose was significantly (P < 0.05) reduced. The chemotactic migration in Boyden chambers (10-μm-thick polycarbonate filter; lower surface count technique) was significantly (P < 0.05) enhanced, presumably due to cGH-induced hyperadhesiveness to the lower surface of the filters. The adhesion in albumin-coated microtiter plates and adherence to canine pulmonary fibroblasts were significantly (P < 0.05) increased, and the increased adhesion resulted in a significant (P < 0.01) increase in transendothelial migration using canine jugular vein endothelial cells. The increase in adhesion was associated with a significant increase in CD11b/CD18 expression. Furthermore, intracellular O2 − production was significantly enhanced in response to both phorbol myristate acetate (P < 0.01) and opsonized zymosan (P < 0.05). In the absence of a PMN-stimulating agent, cGH did not influence the effector functions investigated except for an increased expression of CD11b/CD18.

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