Variable region cDNA sequences and characterization of murine anti-human interferon γ receptor monoclonal antibodies that inhibit receptor binding by interferon γ

1995 ◽  
Vol 32 (17-18) ◽  
pp. 1329-1338 ◽  
Author(s):  
Angela Bridges ◽  
Ashley Birch ◽  
Geoffrey Williams ◽  
Michel Aguet ◽  
Daniel Schlatter ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Receptor Binding ◽  
Variable Region ◽  
Interferon Γ ◽  
Human Interferon ◽  
Cdna Sequences

scholarly journals Specificity and VH sequence of two monoclonal antibodies against the N-terminus of dystrophin

1995 ◽  
Vol 309 (1) ◽  
pp. 355-359 ◽  
Author(s):  
G E Morris ◽  
C Nguyen ◽  
Nguyen thi Man
Keyword(s):  
Monoclonal Antibodies ◽  
Point Mutations ◽  
Hypervariable Region ◽  
Variable Region ◽  
Binding Studies ◽  
Cdna Sequences ◽  
N Terminus ◽  
Random Library ◽  
Blast Cells

We have used a random library of 15-mer peptides expressed on phage to show that two monoclonal antibodies (mAbs) require only the first three amino acids of dystrophin (Leu-Trp-Trp) for binding. Since the mAbs recognize dystrophin in frozen muscle sections, the results suggest that this hydrophobic N-terminus of dystrophin is accessible to antibody in situ. Quantitative binding studies suggested minor differences in specificity between the two mAbs, so the Ig heavy-chain variable region (VH) sequences of the two hybridomas were determined by RT-PCR and cDNA sequencing. After elimination of PCR errors, the two cDNA sequences were found to be identical except for five somatic mutations which resulted in three amino acid changes in the second hypervariable region (CDR2). The results suggest that the two hybridomas originated from the same lymphocyte clone in a germinal centre of the spleen, but underwent different point mutations and subtype switches during clonal expansion to form blast cells.

Generation and Characterization of Monoclonal Antibodies against Human Interferon-lambda1

2008 ◽  
Vol 8 (1) ◽  
pp. 7
Author(s):  
Seung-Ho Hong ◽  
Jung-Sik Kim ◽  
Sun Park
Keyword(s):  
Monoclonal Antibodies ◽  
Human Interferon

Production, purification and characterization of recombinant human interferon γ

1992 ◽  
Vol 604 (1) ◽  
pp. 143-155 ◽  
Author(s):  
Z. Zhang ◽  
K.-T. Tong ◽  
M. Belew ◽  
T. Pettersson ◽  
J.-C. Janson
Keyword(s):  
Interferon Γ ◽  
Human Interferon ◽  
Purification And Characterization

scholarly journals Production and characterization of anti-human interferon γ receptor antibody fragments that inhibit cytokine binding to the receptor

1996 ◽  
Vol 9 (4) ◽  
pp. 365-370 ◽  
Author(s):  
Angela Bridges ◽  
Fiona Stuart ◽  
Julia Späth ◽  
Stefan Lang ◽  
Christoph Henke ◽  
...  
Keyword(s):  
Interferon Γ ◽  
Antibody Fragments ◽  
Human Interferon ◽  
Receptor Antibody
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