Intracellular survival of Brucella abortus, Mycobacterium bovis BCG, Salmonella dublin, and Salmonella typhimurium in macrophages from cattle genetically resistant to Brucella abortus

1996 ◽  
Vol 50 (1-2) ◽  
pp. 55-65 ◽  
Author(s):  
T. Qureshi ◽  
J.W. Templeton ◽  
L.G. Adams
Keyword(s):  
Salmonella Typhimurium ◽  
Mycobacterium Bovis ◽  
Brucella Abortus ◽  
Intracellular Survival ◽  
Mycobacterium Bovis Bcg ◽  
Salmonella Dublin

scholarly journals Dissecting the Mycobacterium bovis BCG Response to Macrophage Infection to Help Prioritize Targets for Anti-Tuberculosis Drug and Vaccine Discovery

Vaccines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 113
Author(s):  
Jamie Medley ◽  
Aaron Goff ◽  
Paulo J. G. Bettencourt ◽  
Madelaine Dare ◽  
Liam Cole ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Iron Homeostasis ◽  
Vaccine Development ◽  
Gene Families ◽  
Intracellular Survival ◽  
Macrophage Infection ◽  
Mycobacterium Bovis Bcg ◽  
Macrophage Phagocytosis ◽  
Upregulated Genes ◽  
Novel Drug

New strategies are required to reduce the worldwide burden of tuberculosis. Intracellular survival and replication of Mycobacterium tuberculosis after macrophage phagocytosis is a fundamental step in the complex host–pathogen interactions that lead to granuloma formation and disease. Greater understanding of how the bacterium survives and thrives in these environments will inform novel drug and vaccine discovery programs. Here, we use in-depth RNA sequencing of Mycobacterium bovis BCG from human THP-1 macrophages to describe the mycobacterial adaptations to the intracellular environment. We identify 329 significantly differentially regulated genes, highlighting cholesterol catabolism, the methylcitrate cycle and iron homeostasis as important for mycobacteria inside macrophages. Examination of multi-functional gene families revealed that 35 PE/PPE genes and five cytochrome P450 genes were upregulated 24 h after infection, highlighting pathways of potential significance. Comparison of the intracellular transcriptome to gene essentiality and immunogenicity studies identified 15 potential targets that are both required for intracellular survival and induced on infection, and eight upregulated genes that have been demonstrated to be immunogenic in TB patients. Further insight into these new and established targets will support drug and vaccine development efforts.

The role of histamine in the intracellular survival of Mycobacterium bovis BCG

2006 ◽  
Vol 8 (4) ◽  
pp. 1035-1044 ◽  
Author(s):  
Klára Megyeri ◽  
Krisztina Buzás ◽  
András Miczák ◽  
Edit Buzás ◽  
Lóránd Kovács ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Intracellular Survival ◽  
Mycobacterium Bovis Bcg

scholarly journals Anti-dormant Mycobacterial Activity of Viomellein and Xanthomegnin, Naphthoquinone Dimers Produced by Marine-derived Aspergillus sp

2017 ◽  
Vol 12 (4) ◽  
pp. 1934578X1701200
Author(s):  
Kentaro Kamiya ◽  
Masayoshi Arai ◽  
Andi Setiawan ◽  
Motomasa Kobayashi
Keyword(s):  
Mycobacterium Bovis ◽  
Plasmid Dna ◽  
Active Fraction ◽  
Mycobacterium Bovis Bcg ◽  
Mycobacterial Genome ◽  
Weak Activity ◽  
Aspergillus Sp ◽  
Chelating Effect

In the course of a search for anti-dormant mycobacterial substances from marine-derived microorganisms, viomellein (1) and xanthomegnin (2) were rediscovered from the active fraction of the culture of a marine-derived Aspergillus sp. together with rubrosulphin (3) and asteltoxin (4) on the guidance of bioassay-guided separation. In particular, compound 1 showed higher activity against the dormant than against actively growing Mycobacterium bovis BCG and weak activity against M. smegmatis. Furthermore, evidence that compound 1 did not directly bind to plasmid DNA suggests its anti-mycobacterial activity differs from its direct chelating effect on the mycobacterial genome.

Severe Mycobacterium bovis BCG infections in a large series of novel IL–12 receptor β1 deficient patients and evidence for the existence of partial IL–12 receptor β1 deficiency

2003 ◽  
Vol 33 (1) ◽  
pp. 59-69 ◽  
Author(s):  
Elgin G. R. Lichtenauer-Kaligis ◽  
Tjitske de Boer ◽  
Frank A. W. Verreck ◽  
Sjaak van Voorden ◽  
Marieke A. Hoeve ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Large Series ◽  
Mycobacterium Bovis Bcg
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