The detection and differentiation of foot-and-mouth disease viruses using solid-phase nucleic acid hybridization

1990 ◽  
Vol 27 (2) ◽  
pp. 175-188 ◽  
Author(s):  
Robin G. McFarlane ◽  
Thomas W. Molitor ◽  
Vikram N. Vakharia
Keyword(s):  
Nucleic Acid ◽  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Nucleic Acid Hybridization ◽  
Foot And Mouth

A solid-phase blocking ELISA for detection of type O foot-and-mouth disease virus antibodies suitable for mass serology

2003 ◽  
Vol 107 (1) ◽  
pp. 89-98 ◽  
Author(s):  
Gilles Chénard ◽  
Kor Miedema ◽  
Peter Moonen ◽  
Remco S Schrijver ◽  
Aldo Dekker
Keyword(s):  
Disease Virus ◽  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Blocking Elisa ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Seroprevalence of Foot and Mouth Disease Virus Infection in Some Wildlife and Cattle in Bauchi State, Nigeria

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Y. J. Atuman ◽  
C. A. Kudi ◽  
P. A. Abdu ◽  
O. O. Okubanjo ◽  
A. Abubakar ◽  
...  
Keyword(s):  
South Africa ◽  
Disease Virus ◽  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Blood Collection ◽  
System Control ◽  
High Morbidity ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Foot and mouth disease (FMD) is an important transboundary viral disease of both domestic and wild cloven-hoofed animals characterized by high morbidity with devastating consequence on the livestock worldwide. Despite the endemic nature of FMD in Nigeria, little is known about the epidemiology of the disease at the wildlife-livestock interface level. To address this gap, blood samples were collected between 2013 and 2015 from some wildlife and cattle, respectively, within and around the Yankari Game Reserve and Sumu Wildlife Park in Bauchi State, Nigeria. Wild animals were immobilized using a combination of etorphine hydrochloride (M99® Krüger-Med South Africa) at 0.5–2 mg/kg and azaperone (Stresnil®, Janssen Pharmaceuticals (Pty.) Ltd., South Africa) at 0.1 mg/kg using a Dan-Inject® rifle (Dan-Inject APS, Sellerup Skovvej, Denmark) fitted with a 3 ml dart syringe and for reversal, naltrexone (Trexonil® Kruger-Med South Africa) at 1.5 mg IM was used, and cattle were restrained by the owners for blood collection. Harvested sera from blood were screened for presence of antibodies against the foot and mouth disease virus (FMDV) using the PrioCHECK® 3ABC NSP ELISA kit, and positive samples were serotyped using solid-phase competitive ELISA, (IZSLER Brescia, Italy). Out of the 353 sera collected from cattle and wildlife 197 (65.7%) and 13 (24.5%) (P<0.05), respectively, tested positive for antibodies to the highly conserved nonstructural 3ABC protein of FMDV by the FMDV-NS blocking ELISA. Classification of cattle into breed and sex showed that detectable antibodies to FMDV were higher (P<0.05) in White Fulani 157 (72.8%) than in Red Bororo 23 (39.7%) and Sokoto Gudali 17 (33.3%) breeds of cattle, whereas in females, detectable FMDV antibodies were higher (P<0.05) 150 (72.8%) than in males 47 (50.0%). In the wildlife species, antibodies to FMDV were detected in the waterbucks 2 (28.6%), elephant 1 (25.0%), wildebeests 4 (33.3%), and elands 6 (25.0%). Four serotypes of FMDV: O, A, SAT 1, and SAT 2 were detected from the 3ABC positive reactors in waterbucks, elephants, wildebeests, and elands. The results showed presence of antibodies to FMDV in some wildlife and cattle and suggested that wildlife could equally play an important role in the overall epidemiology of FMD in Nigeria. FMD surveillance system, control, and prevention program should be intensified in the study area.

Validation of a foot-and-mouth disease antibody screening solid-phase competition ELISA (SPCE)

2004 ◽  
Vol 115 (2) ◽  
pp. 145-158 ◽  
Author(s):  
G.A. Paiba ◽  
J. Anderson ◽  
D.J. Paton ◽  
A.W. Soldan ◽  
S. Alexandersen ◽  
...  
Keyword(s):  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Antibody Screening ◽  
Phase Competition ◽  
Competition Elisa ◽  
Foot And Mouth ◽  
Disease Antibody

scholarly journals The specific detection of foot-and-mouth disease virus whole particle antigen (140S) by enzyme labelled immunosorbent assay

1979 ◽  
Vol 83 (1) ◽  
pp. 127-134 ◽  
Author(s):  
E. M. E. Abu Elzein ◽  
J. R. Crowther
Keyword(s):  
Guinea Pig ◽  
Disease Virus ◽  
Immunosorbent Assay ◽  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Specific Detection ◽  
Sandwich Technique ◽  
Mouth Disease Virus ◽  
Foot And Mouth

SUMMARYA solid-phase micro-enzyme-labelled immunosorbent assay (ELISA) using guinea pig antiserum against purified (140S) inactivated foot-and-mouth disease (FMD) virus has been usedin a sandwich technique to specifically measure 140S virus in the presence of 12S material.

scholarly journals Serological response of commercial dairy cattle to inactivated foot-and-mouth disease vaccine (type-O & A) in Nigeria

2015 ◽  
Vol 1 (2) ◽  
pp. 163-168 ◽  
Author(s):  
David Dazhia Lazarus ◽  
Yiltawe Simwal Wungak ◽  
Mohammed Ignatius Adah ◽  
John Okpapi Ibu ◽  
Jerry Ngutor Abenga ◽  
...  
Keyword(s):  
Immune Response ◽  
Dairy Cattle ◽  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Serological Response ◽  
Post Vaccination ◽  
Vaccine Type ◽  
Foot And Mouth ◽  
The Mean

An observational study was conducted in a peri-urban dairy establishment in Jos South, Plateau State Nigeria to determine immune response of dairy cattle to commercial inactivated foot-and-mouth disease vaccine serotypes (O and A). Thirty seven Friesian cattle aged ?2years old with their crosses (15 selected pre-vaccination and 22 selected 21 days post-vaccination) were investigated for immune response to vaccination with an inactivated trivalent FMD vaccine containing serotypes O, A and SAT 2). Sera collected on day 0 pre-vaccination and 21 days post-vaccination was tested for structural protein antibodies to FMD serotypes O and A using the Solid Phase Competitive ELISA assay. The mean OD value for serum end point titre of FMD serotype O pre-vaccination was 11.64% with 6.67% (95%CI: 0.33 – 28.73) of the selected cattle being seropositive, at 21 days post-vaccination the mean OD value in selected cattle was 52.83% with 68.18% (95%CI: 46.95 – 84.89) of the selected cattle seropositive. For the FMD serotype A, 26.67% (95%CI: 9.10 – 52.53) of the selected cattle were seropositive pre-vaccination with a mean OD value of 29.21% and by 21 days post-vaccination, 72.73% (95%CI: 51.67 – 88.13) of the selected cattle were seropositive with a mean OD value of 61.70%. Serological response to vaccination improved in most selected cattle by 21 days post-vaccination. This study result has indicated that commercial inactivated FMD vaccines used for the prophylactic control of FMD in commercial dairy farm in Nigeria provoked immune response after a single shot.Asian J. Med. Biol. Res. June 2015, 1(2): 163-168

scholarly journals Detection and quantification of IgM, IgA, IgG1 and IgG2 antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay

1981 ◽  
Vol 86 (1) ◽  
pp. 79-85 ◽  
Author(s):  
E. M. E. Abu Elzein ◽  
J. R. Crowther
Keyword(s):  
Serum Proteins ◽  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Class ◽  
Fmd Virus ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Preliminary Separation

SUMMARYA simple solid-phase enzyme immunoassay is described for the detection of antibody classes showing activity against foot-and-mouth disease (FMD) virus in bovine sera. The assay achieves a preliminary separation of the specific class of antibody from other serum proteins through immuno-adsorption to class-specific immunoglobulin-coated wells of micro-titre plates. The specific antibody is reacted with FMD virus, which is then detected by an enzyme-labelled anti virus IgG.

scholarly journals Serological evaluation for the current epidemic situation of foot and mouth disease among cattle and buffaloes in Egypt

2020 ◽  
Vol 13 (1) ◽  
pp. 1-9
Author(s):  
Mariam M. Abd El-Rhman ◽  
Diea G. Abo El-Hassan ◽  
Walid S. Awad ◽  
Sayed A. H. Salem
Keyword(s):  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Elisa Test ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Structural Protein ◽  
Fmdv Serotypes ◽  
Foot And Mouth ◽  
Trivalent Vaccine

Aim: The present study was aimed to investigate the epidemic situation of foot-and-mouth disease (FMD) in Egypt from 2016 to 2018 based on the detection of FMD virus (FMDV) in carrier or previously infected animals, by determination of antibodies against non-structural protein (NSP), implementation a pilot study on circulating FMDV serotypes and assure the efficacy of locally produced inactivated trivalent vaccine. Materials and Methods: A total of 1500 sera were collected from apparent healthy vaccinated cattle and buffaloes from three Egyptian geographical sectors, representing ten governorates. Determination of FMD antibodies against NSP was carried out using 3ABC enzyme-linked immunosorbent assay (ELISA) test. Serotyping of the circulating FMDV and assure the vaccine efficacy was performed using solid-phase competitive ELISA. Results: The 3ABC ELISA test revealed 26.4% and 23.7% positive for FMDV-NSP antibodies in cattle and buffalo sera, respectively. The highest positivity was in Delta Sector among both cattle 42.3% and buffaloes 28.8%. Serotyping of FMDV-positive NSP sera in El-Qalyubia Governorate for the circulating FMDV serotypes O, A, and Southern African Territories (SAT) 2 was 52.2%, 17.4%, and 30.4% in cattle and 31.8%, 27.3%, and 40.9% in buffaloes, respectively. The overall protection level due to the vaccination program was 62.1 and 60.9% in cattle and buffaloes, respectively, while the protective level of the FMDV serotypes O, A, and SAT2 included in the inactivated trivalent vaccine was 73.9, 84.6, and 63.8% in cattle and 72.3, 82.3, and 63.5% in buffaloes, respectively. Conclusion: The present study recommended full determination for the immunogenic relationship between the vaccine strains and the field strains to attain maximum protection against the circulating viruses.

scholarly journals Comparison of two ELISA methods for detection of antibodies against Footand- Mouth Disease virus of cattle breeds in Turkey

2018 ◽  
Vol 67 (1) ◽  
pp. 33
Author(s):  
Ö. B. INCE ◽  
R. KALKAN ◽  
S. ÇAKIR
Keyword(s):  
Disease Virus ◽  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Serotype A ◽  
Cattle Breeds ◽  
Serotype O ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
History Of

The study was conducted using two ELISA methods - the liquid phase blocking ELISA (LPBE) and solid phase competition ELISA (SPCE) for the detection of foot-and-mouth disease virus (FMDV) serotype A- and O-specific antibodies of different cattle breeds in Turkey. These methods were compared in 426 cattle previously vaccinated with oil-adjuvanted bivalent vaccine as well as in sera from 40 cattle with no history of foot-and-mouth disease infection or vaccination. The results were found that SPCE had a better specificity (serotype A; 100% and serotype O; 97.50%) than LPBE (serotype A 95.00% and serotype O 92.50%). Sensitivity of SPCE had also better values (serotype A; 99.30% and serotype O; 98.59%) than LPBE (serotype A; 97.89% and serotype O; 96.48%). The results of the present study showed that the SPCE method is more reliable than LPBE.

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