scholarly journals Serological evaluation for the current epidemic situation of foot and mouth disease among cattle and buffaloes in Egypt

2020 ◽  
Vol 13 (1) ◽  
pp. 1-9
Author(s):  
Mariam M. Abd El-Rhman ◽  
Diea G. Abo El-Hassan ◽  
Walid S. Awad ◽  
Sayed A. H. Salem
Keyword(s):  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Elisa Test ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Structural Protein ◽  
Fmdv Serotypes ◽  
Foot And Mouth ◽  
Trivalent Vaccine

Aim: The present study was aimed to investigate the epidemic situation of foot-and-mouth disease (FMD) in Egypt from 2016 to 2018 based on the detection of FMD virus (FMDV) in carrier or previously infected animals, by determination of antibodies against non-structural protein (NSP), implementation a pilot study on circulating FMDV serotypes and assure the efficacy of locally produced inactivated trivalent vaccine. Materials and Methods: A total of 1500 sera were collected from apparent healthy vaccinated cattle and buffaloes from three Egyptian geographical sectors, representing ten governorates. Determination of FMD antibodies against NSP was carried out using 3ABC enzyme-linked immunosorbent assay (ELISA) test. Serotyping of the circulating FMDV and assure the vaccine efficacy was performed using solid-phase competitive ELISA. Results: The 3ABC ELISA test revealed 26.4% and 23.7% positive for FMDV-NSP antibodies in cattle and buffalo sera, respectively. The highest positivity was in Delta Sector among both cattle 42.3% and buffaloes 28.8%. Serotyping of FMDV-positive NSP sera in El-Qalyubia Governorate for the circulating FMDV serotypes O, A, and Southern African Territories (SAT) 2 was 52.2%, 17.4%, and 30.4% in cattle and 31.8%, 27.3%, and 40.9% in buffaloes, respectively. The overall protection level due to the vaccination program was 62.1 and 60.9% in cattle and buffaloes, respectively, while the protective level of the FMDV serotypes O, A, and SAT2 included in the inactivated trivalent vaccine was 73.9, 84.6, and 63.8% in cattle and 72.3, 82.3, and 63.5% in buffaloes, respectively. Conclusion: The present study recommended full determination for the immunogenic relationship between the vaccine strains and the field strains to attain maximum protection against the circulating viruses.

scholarly journals Pan-Serotype Diagnostic for Foot-and-Mouth Disease Using the Consensus Antigen of Nonstructural Protein 3B

2015 ◽  
Vol 53 (6) ◽  
pp. 1797-1805 ◽  
Author(s):  
Alyssa K. Van Dreumel ◽  
Wojtek P. Michalski ◽  
Leanne M. McNabb ◽  
Brian J. Shiell ◽  
Nagendrakumar B. Singanallur ◽  
...  
Keyword(s):  
Amino Acid ◽  
Diagnostic Test ◽  
Nonstructural Protein ◽  
Consensus Sequence ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Amino Acid Consensus Sequence ◽  
Fmdv Serotypes ◽  
Foot And Mouth

An amino acid consensus sequence for the seven serotypes of foot-and-mouth disease virus (FMDV) nonstructural protein 3B, including all three contiguous repeats, and its use in the development of a pan-serotype diagnostic test for all seven FMDV serotypes are described. The amino acid consensus sequence of the 3B protein was determined from a multiple-sequence alignment of 125 sequences of 3B. The consensus 3B (c3B) protein was expressed as a soluble recombinant fusion protein with maltose-binding protein (MBP) using a bacterial expression system and was affinity purified using amylose resin. The MBP-c3B protein was used as the antigen in the development of a competition enzyme-linked immunosorbent assay (cELISA) for detection of anti-3B antibodies in bovine sera. The comparative diagnostic sensitivity and specificity at 47% inhibition were estimated to be 87.22% and 93.15%, respectively. Reactivity of c3B with bovine sera representing the seven FMDV serotypes demonstrated the pan-serotype diagnostic capability of this bioreagent. The consensus antigen and competition ELISA are described here as candidates for a pan-serotype diagnostic test for FMDV infection.

scholarly journals Detection and quantification of IgM, IgA, IgG1 and IgG2 antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay

1981 ◽  
Vol 86 (1) ◽  
pp. 79-85 ◽  
Author(s):  
E. M. E. Abu Elzein ◽  
J. R. Crowther
Keyword(s):  
Serum Proteins ◽  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Class ◽  
Fmd Virus ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Preliminary Separation

SUMMARYA simple solid-phase enzyme immunoassay is described for the detection of antibody classes showing activity against foot-and-mouth disease (FMD) virus in bovine sera. The assay achieves a preliminary separation of the specific class of antibody from other serum proteins through immuno-adsorption to class-specific immunoglobulin-coated wells of micro-titre plates. The specific antibody is reacted with FMD virus, which is then detected by an enzyme-labelled anti virus IgG.

scholarly journals Simple and Rapid Lateral-Flow Assay for the Detection of Foot-and-Mouth Disease Virus

2009 ◽  
Vol 16 (11) ◽  
pp. 1660-1664 ◽  
Author(s):  
Jae Ku Oem ◽  
Nigel P. Ferris ◽  
Kwang-Nyeong Lee ◽  
Yi-Seok Joo ◽  
Bang-Hun Hyun ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Lateral Flow ◽  
Mouth Disease ◽  
Lateral Flow Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fmdv Serotypes ◽  
Mouth Disease Virus ◽  
Field Samples ◽  
Foot And Mouth

ABSTRACT A simple lateral-flow assay (LFA) based on a monoclonal antibody (MAb 70-17) was developed for the detection of foot-and-mouth disease virus (FMDV) under nonlaboratory conditions. The LFA was evaluated with epithelial suspensions (n = 704) prepared from current and historical field samples which had been submitted to the Pirbright Laboratory (United Kingdom) and from negative samples (n = 100) collected from naïve animals in Korea. Four FMDV serotypes (type O, A, Asia 1, and C) were detected in the LFA, but not the remaining three FMDV serotypes (SAT 1, SAT 2, and SAT 3). The diagnostic sensitivity of the LFA for FMDV types O, A, C, and Asia 1 was similar, at approximately 87.3%, to that of 87.7% obtained with antigen enzyme-linked immunosorbent assay (Ag-ELISA). The diagnostic specificity of the LFA was 98.8%, compared to 100% for the Ag-ELISA. These results demonstrate that the LFA using the FMDV MAb 70-17 to detect FMDV is a supportive method for taking rapid measurements at the site of a suspected foot-and-mouth disease outbreak in Asia before diagnosing the disease in the laboratory, thereby offering the possibility of implementing control procedures more rapidly.

scholarly journals Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 301
Author(s):  
Quyen Thi Nguyen ◽  
Jihyun Yang ◽  
Jae-Won Byun ◽  
Hyun Mi Pyo ◽  
Mi-Young Park ◽  
...  
Keyword(s):  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Type A ◽  
Cross Reactivity ◽  
Mouth Disease ◽  
Fmdv Serotypes ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Diagnostic Approaches ◽  
Fmdv Type

Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease affecting cloven-hoofed livestock worldwide. FMD virus (FMDV) type A is one of the most common causes of FMD outbreaks among the seven FMDV serotypes, and its serological diagnosis is therefore important to confirm FMDV type A infection and to determine FMD vaccine efficacy. Here, we generated monoclonal antibodies (mAbs) specific to FMDV type A via hybridoma systems using an inactivated FMDV type A (A22/Iraq/1964) and found 4 monoclones (#29, #106, #108, and #109) with high binding reactivity to FMDV type A among 594 primary clones. In particular, the #106 mAb had a higher binding reactivity to the inactivated FMDV type A than the other mAbs and a commercial mAb. Moreover, the #106 mAb showed no cross-reactivity to inactivated FMDV type South African territories 1, 2, and 3, and low reactivity to inactivated FMDV type O (O1 Manisa). Importantly, the solid-phase competitive ELISA (SPCE) using horseradish peroxidase (HRP)-conjugated #106 mAb detected FMDV type A-specific Abs in sera from FMD type A-vaccinated cattle more effectively than a commercial SPCE. These results suggest that the newly developed FMDV type A-specific mAb might be useful for diagnostic approaches for detecting Abs against FMDV type A.

scholarly journals Preparation of Foot and Mouth Disease trivalent vaccine type A, O, SAT2 and determination of the Guinea pig protective dose 50 (GPPD50)

2013 ◽  
Vol 6 (11) ◽  
pp. 844-851 ◽  
Author(s):  
Hind M. Daoud ◽  
Ehab El-Sayed Ibrahim ◽  
Wael Mossad Gamal El-Din ◽  
Amr Ismail Hassan Hassanin
Keyword(s):  
Guinea Pig ◽  
Foot And Mouth Disease ◽  
Type A ◽  
Mouth Disease ◽  
Vaccine Type ◽  
Foot And Mouth ◽  
Trivalent Vaccine

scholarly journals Sero Survey of Foot and Mouth Disease Virus Infection in Cattle Crossing Some Major Border States in Northwestern Nigeria

2017 ◽  
Vol 61 (3) ◽  
pp. 12-18
Author(s):  
D. Babangida ◽  
A. A. Ibrahim ◽  
F. O. Oladayo ◽  
A. A. Magaji ◽  
B. R. Alkali ◽  
...  
Keyword(s):  
Relative Risk ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Sub Saharan Africa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Structural Protein ◽  
Chi Square ◽  
Border States ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Abstract Foot and mouth disease (FMD) poses a major constraint to international trade in animals and animal products in sub-Saharan Africa. A retrospective and serological survey was conducted in two major Border States of Sokoto and Kebbi in north-western Nigeria. This study was aimed at determining the sero-prevalence of FMD virus (FMDV) antibodies in cattle at international animal control posts and to examine cattle population movement across the border area for a period of one year (January to December 2014) from the available records. Eight hundred and eighty (880) sera samples were collected and screened for the presence of antibodies to FMDV using the competitive enzyme linked immunosorbent assay (ELISA) technique. The data were subjected to chi-square and relative risk to check for independence and association. An overall seropositive rate was found to be 55.2 % (486/880). A 79.9 % (359/450) sero-positive rate was obtained from the Kamba border, while 29.5 % (127/430) was found at the Illela border. Kamba showed a statistically significant (P < 0.05) higher sero-prevalence when compared with cattle that are crossing the Illela border (Relative Risk 2.70; 95 % Confidence Interval 2.317—3.149). Retrospective data from the control posts revealed that an average number of 2019 and 2747 of cattle, respectively, crossed the Kamba and Illela international borders monthly. The highest influx of animals from the Niger Republic through the Illela international border was encountered between the month of March and April 2014. The magnitude of the presence of FMDV Non-structural protein (NSP) antibodies in the study areas is an indication of the infection and the presence of the virus in the study areas and the neighbouring countries.

scholarly journals Effect of data quality on estimates of farm infectiousness trends in the UK 2001 foot-and-mouth disease epidemic

2006 ◽  
Vol 4 (13) ◽  
pp. 235-241 ◽  
Author(s):  
Nicholas J Savill ◽  
Darren J Shaw ◽  
Rob Deardon ◽  
Michael J Tildesley ◽  
Matthew J Keeling ◽  
...  
Keyword(s):  
Mathematical Models ◽  
Data Quality ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Inaccurate Data ◽  
Foot And Mouth ◽  
The Uk ◽  
Parameter Values

Most of the mathematical models that were developed to study the UK 2001 foot-and-mouth disease epidemic assumed that the infectiousness of infected premises was constant over their infectious periods. However, there is some controversy over whether this assumption is appropriate. Uncertainty about which farm infected which in 2001 means that the only method to determine if there were trends in farm infectiousness is the fitting of mechanistic mathematical models to the epidemic data. The parameter values that are estimated using this technique, however, may be influenced by missing and inaccurate data. In particular to the UK 2001 epidemic, this includes unreported infectives, inaccurate farm infection dates and unknown farm latent periods. Here, we show that such data degradation prevents successful determination of trends in farm infectiousness.

scholarly journals Use of a Standardized Bovine Serum Panel To Evaluate a Multiplexed Nonstructural Protein Antibody Assay for Serological Surveillance of Foot-and-Mouth Disease

2007 ◽  
Vol 14 (11) ◽  
pp. 1472-1482 ◽  
Author(s):  
Julie Perkins ◽  
Satya Parida ◽  
Alfonso Clavijo
Keyword(s):  
Nonstructural Protein ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reference Laboratory ◽  
Additional Information ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Antibody Assays ◽  
Serological Surveillance

ABSTRACT Liquid array technology has previously been used to show proof of principle of a multiplexed nonstructural protein serological assay to differentiate foot-and-mouth disease virus-infected and vaccinated animals. The current multiplexed assay consists of synthetically produced peptide signatures 3A, 3B, and 3D and the recombinant protein signature 3ABC in combination with four controls. To determine the diagnostic specificity of each signature in the multiplex, the assay was evaluated against a naive population (n = 104) and a vaccinated population (n = 94). Subsequently, the multiplexed assay was assessed by using a panel of bovine sera generated by the World Reference Laboratory for foot-and-mouth disease in Pirbright, United Kingdom. This serum panel has been used to assess the performance of other singleplex enzyme-linked immunosorbent assay (ELISA)-based nonstructural protein antibody assays. The 3ABC signature in the multiplexed assay showed performance comparable to that of a commercially available nonstructural protein 3ABC ELISA (Cedi test), and additional information pertaining to the relative diagnostic sensitivity of each signature in the multiplex was acquired in one experiment. The encouraging results of the evaluation of the multiplexed assay against a panel of diagnostically relevant samples promote further assay development and optimization to generate an assay for routine use in foot-and-mouth disease serological surveillance.

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