Detection of bovine herpesvirus-1 in bovine semen by a nested PCR assay

1993 ◽  
Vol 44 (1) ◽  
pp. 129-139 ◽  
Author(s):  
Martin Wiedmann ◽  
Richard Brandon ◽  
Pat Wagner ◽  
Edward J. Dubovi ◽  
Carl A. Batt
Keyword(s):  
Nested Pcr ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Herpesvirus 1

scholarly journals Neurological disorder in cattle associated with bovine herpesvirus 4

2011 ◽  
Vol 63 (4) ◽  
pp. 828-835 ◽  
Author(s):  
E.A. Costa ◽  
A.C. Vasconcelos ◽  
M.R.Q. Bomfim ◽  
H.B. Amorim ◽  
G.B.L. Lima ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Clinical Course ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus Type ◽  
Neurological Signs ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Herpesvirus 4

A nested PCR assay was used to diagnose bovine encephalitis through herpesviruses including bovine herpesvirus 5 (BHV-5), bovine herpesvirus 1 (BHV-1), Aujeszky's disease virus (SHV-1), and ovine herpesvirus 2 (OHV-2) in 14 fragments of central nervous system (CNS) from cattle that died with neurological signs. In addition, as some samples of bovine herpesvirus type 4 (BHV-4) have been isolated from neural tissue, it was also tested by nested PCR. The cases of encephalitis occurred in isolation at different times of the year and did not present any seasonality. The duration of the clinical course ranged between 1 to 15 days, and in 64.3% of the cases it manifested between 1 to 2 days. The most frequently observed neurological signs were ataxia, recumbency, unsteadiness and inability to stand, opisthotonus, paddling movements, nystagmus and ptyalism. In the nested assay, there was no evidence of: BHV-1, SHV-1 or OHV-2 in the DNA obtained from the CNS in any of the samples. But the presence of BHV-4 was found in all fragments of the CNS in cattle which died presenting neurological signs. Moreover, BHV-5 was found in association with BHV-4 in two of these samples.

An international inter-laboratory ring trial to evaluate a real-time PCR assay for the detection of bovine herpesvirus 1 in extended bovine semen

2008 ◽  
Vol 126 (1-3) ◽  
pp. 11-19 ◽  
Author(s):  
Jianning Wang ◽  
Joseph O’Keefe ◽  
Della Orr ◽  
Leo Loth ◽  
Malcolm Banks ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Ring Trial ◽  
Herpesvirus 1

Validation of a real-time PCR assay for the detection of bovine herpesvirus 1 in bovine semen

2007 ◽  
Vol 144 (1-2) ◽  
pp. 103-108 ◽  
Author(s):  
Jianning Wang ◽  
Joseph O’Keefe ◽  
Della Orr ◽  
Leo Loth ◽  
Malcolm Banks ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Herpesvirus 1

A polymerase chain reaction (PCR) assay for the detection of bovine herpesvirus 1 (BHV1) in selectively digested whole bovine semen

1996 ◽  
Vol 20 (4) ◽  
pp. 401-408 ◽  
Author(s):  
L. H. A. Wagter ◽  
R. D. Glas ◽  
N. Bleumink-Pluym ◽  
F. A. C. Van Engelenburg ◽  
F. A. M. Rijsewijk ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bovine Herpesvirus ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Herpesvirus 1 ◽  
Polymerase Chain

EvaGreen-based Multiplex Real-time PCR Assay for Rapid Differentiation of Wild-Type and Glycoprotein E-Deleted Bovine Herpesvirus-1 Strains

2017 ◽  
Vol 28 (4) ◽  
pp. 248-252 ◽  
Author(s):  
Sachin S. Pawar ◽  
Chetan D. Meshram ◽  
Niraj K. Singh ◽  
Mohini Saini ◽  
B. P. Mishra ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Wild Type ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1

scholarly journals Evaluation of a specialized filter-paper matrix for transportation of extended bovine semen to screen for bovine herpesvirus-1 by real-time PCR

2018 ◽  
Vol 257 ◽  
pp. 1-6 ◽  
Author(s):  
Laxmi Narayan Sarangi ◽  
Thodangala Naveena ◽  
Samir Kumar Rana ◽  
Kota Sri Naga Leela Surendra ◽  
Rachamreddy Venkata Chandrasekhar Reddy ◽  
...  
Keyword(s):  
Real Time ◽  
Filter Paper ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Herpesvirus 1

scholarly journals First Report on the Detection and Molecular Characterization of Bovine Herpesvirus 1 from a Clinical case of Infectious Bovine Rhinotracheitis in Pakistan

2021 ◽  
Vol 41 (01) ◽  
pp. 160-162
Author(s):  
Aayesha Riaz
Keyword(s):  
Molecular Characterization ◽  
Nested Pcr ◽  
Bovine Herpesvirus ◽  
Nasal Discharge ◽  
Infectious Bovine Rhinotracheitis ◽  
Bovine Herpesvirus 1 ◽  
Nasal Swabs ◽  
Herpesvirus 1 ◽  
First Time

Bovine herpesvirus 1 (BHV-1) is a noteworthy reason for many Cattle/Buffalo diseases. Infectious bovine rhinotracheitis (IBR) is one of the diseases which are caused by the BHV-1. In the present study a cow which was suspected of IBR was examined. The animal was suffering from fever and respiratory distress along with rhinitis (red nose), in appetence, and dyspnea. The nasal mucosa and muzzle were distinctly inflamed, with nasal discharge. DNA from blood samples and nasal swabs were subjected to nested PCR using glycoprotein B gene (gB) Primers. The samples were found positive for BHV-1 gB gene. Sequencing and phylogenetic analysis revealed close similarities with other BHV-1 gB gene sequences. The accession numbers assigned to this pioneer sequences in GenBank are MT449510 (BHV-1-Pak 1) and MT449511 (BHV-1-Pak 2). In this study, we reported for the first time the detection of DNA of BHV-1 through nested PCR assay and molecular characterization of BHV-1 gB gene in Pakistan. This study will be useful in further diagnoses of BHV-1 in Pakistan and in development of BHV-1 vaccine to reduce economical losses due to IBR

Rapid detection of bovine herpesvirus 1 in bovine semen by loop-mediated isothermal amplification (LAMP) assay

2013 ◽  
Vol 159 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Sachin S. Pawar ◽  
Chetan D. Meshram ◽  
Niraj K. Singh ◽  
Arvind A. Sonwane ◽  
Mohini Saini ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Lamp Assay ◽  
Bovine Herpesvirus ◽  
Isothermal Amplification ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Loop Mediated Isothermal Amplification ◽  
Herpesvirus 1
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