Detection of Borna disease virus RNA in naturally infected animals by a nested polymerase chain reaction

1994 ◽  
Vol 46 (2) ◽  
pp. 133-143 ◽  
Author(s):  
Wolfgang Zimmermann ◽  
Ralf Dürrwald ◽  
Hanns Ludwig
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Borna Disease Virus ◽  
Virus Rna ◽  
Polymerase Chain ◽  
Borna Disease

No borna disease virus-specific RNA detected in blood of race horses and jockeys

2006 ◽  
Vol 18 (3-4) ◽  
pp. 177-180 ◽  
Author(s):  
Yong-Ku Kim ◽  
Kyung-Bo Noh ◽  
Chang-Su Han ◽  
Ju-Young Moon ◽  
Do-Kyung Yoon ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Mononuclear Cells ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Peripheral Blood Mononuclear ◽  
Borna Disease Virus ◽  
Polymerase Chain ◽  
Race Horses ◽  
Borna Disease

Background:Borna disease virus (BDV) predominantly infects horses and sheep, causing a broad range of behavioural disorders. It is controversial whether BDV infects humans and causes psychiatric disorders.Objectives:We searched for BDV-derived nucleic acids in blood of race horses and jockeys riding the horses.Methods:We assayed for the BDV genome in RNA extracted from peripheral blood mononuclear cells (PBMC) of 39 race horses and 48 jockeys. Two polymerase chain reaction protocols [one-tube reverse transcription–polymerase chain reaction (RT-PCR) and two-step RT-PCR] were used to assay BDV p24 and p40 transcripts.Results:The p24 and p40 viral nucleic acid sequences were not detected in the PBMC RNAs from any of the race horses or jockeys.Conclusions:These data do not support an epidemiological association between BDV infection, race horses and humans.

scholarly journals Sensitive and specific detection of hepatitis C virus RNA with nested polymerase chain reaction and digoxigenin labeled cDNA probe.

Kanzo ◽  
1991 ◽  
Vol 32 (2) ◽  
pp. 136-140 ◽  
Author(s):  
Kazuaki CHAYAMA ◽  
Hiromitsu KUMADA ◽  
Satoshi SAITOH ◽  
Yasuji ARASE ◽  
Kenji IKEDA ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cdna Probe ◽  
Specific Detection ◽  
Hepatitis C Virus Rna ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Virus Rna ◽  
Polymerase Chain

scholarly journals Development of a Nested Polymerase Chain Reaction Method to Detect Oncogenic Marek's Disease Virus from Feather Tips

2007 ◽  
Vol 19 (5) ◽  
pp. 471-478 ◽  
Author(s):  
Shiro Murata ◽  
Kyung-Soo Chang ◽  
Sung-Il Lee ◽  
Satoru Konnai ◽  
Misao Onuma ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Highly Virulent ◽  
Meq Gene

For the easy survey of Marek's disease virus (MDV), feather tip–derived DNA from MDV-infected chickens can be used because feather tips are easy to collect and feather follicle epithelium is known to be the only site of productive replication of cell-free MDV. To develop a diagnostic method to differentiate highly virulent strains of MDV from the attenuated MDV vaccine strain, CVI988, which is widely used, nested polymerase chain reaction (PCR) was performed to detect a segment of the meq gene in feather tip samples of chickens experimentally infected with MDV. In chickens infected with Md5, a strain of oncogenic MDV, the meq gene was consistently detected, whereas the L- meq gene, in which a 180–base pair (180-bp) sequence is inserted into the meq gene, was detected in CVI988-infected chickens. Moreover, the meq gene was mainly detected even in chickens co-infected with both Md5 and CVI988. These results suggest that this method is appropriate for the surveillance of the highly virulent MDV infection in the field.

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