scholarly journals Comparison of three template preparation methods for routine detection of beak and feather disease virus and avian polyomavirus with single and nested polymerase chain reaction in clinical specimens

2008 ◽  
Vol 37 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Oldrich Tomasek ◽  
Oldrich Kubicek ◽  
Viktor Tukac
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Preparation Methods ◽  
Clinical Specimens ◽  
Polymerase Chain ◽  
Avian Polyomavirus ◽  
Feather Disease Virus

scholarly journals Psittacine beak and feather disease virus in budgerigars and ring-neck parakeets in South Africa

2004 ◽  
Vol 71 (1) ◽  
Author(s):  
J. Albertyn ◽  
K.M. Tajbhai ◽  
R.R. Bragg
Keyword(s):  
South Africa ◽  
Polymerase Chain Reaction ◽  
Genetic Variation ◽  
Disease Virus ◽  
Common Disease ◽  
Sample Collection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Feather Disease Virus ◽  
Viral Isolates

Psittacine beak and feather disease (PBFD) is a common disease of the psittacine species and is caused by the psittacine beak and feather disease virus (PBFDV). In this study the occurrence of the disease in ring-neck parakeets and budgerigars in South Africa suffering from feathering problems, using polymerase chain reaction as a diagnostic test was investigated. The genetic variation between viral isolates was also studied. Results indicate that PBFDV can be attributed to being the cause of feathering problems in some of the ring-neck parakeets and budgerigars in South Africa. Genetic variation of isolates occurs between species and individuals. A cheap and easy to use method of blood sample collection on filter paper for diagnostic purposes was also evaluated. It proved to be less stressful to the birds and did not inhibit further processes.

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