Utilization of polymerase chain reaction (PCR) for assessment of gamma-interferon (IFN-γ) induction in human peripheral blood lymphocytes (PBL)

1991 ◽  
Vol 32 ◽  
pp. 32
Author(s):  
F. Pazderka ◽  
J. Enns ◽  
V. Ramassar ◽  
P.F. Halloran
Keyword(s):  
Polymerase Chain Reaction ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Gamma Interferon ◽  
Chain Reaction ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Ifn Γ ◽  
Polymerase Chain

scholarly journals Gamma Interferon Is a Major Suppressive Factor Produced by Activated Human Peripheral Blood Lymphocytes That Is Able To Inhibit Foamy Virus-Induced Cytopathic Effects

1999 ◽  
Vol 73 (2) ◽  
pp. 1724-1728 ◽  
Author(s):  
Valeria Falcone ◽  
Matthias Schweizer ◽  
Antonio Toniolo ◽  
Dieter Neumann-Haefelin ◽  
Andreas Meyerhans
Keyword(s):  
Inhibitory Activity ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Foamy Virus ◽  
Peripheral Blood Lymphocytes ◽  
Gamma Interferon ◽  
Blood Lymphocytes ◽  
Cytopathic Effects ◽  
Human Peripheral Blood Lymphocytes ◽  
Ifn Γ

ABSTRACT The activation of human peripheral blood lymphocytes by mitogens or by triggering the T-cell receptor with anti-CD3 antibodies leads to the production of a potent soluble inhibitory activity against foamy virus-induced cytopathic effects in vitro. The inhibitory activity acts in a species-specific manner. As a consequence, the isolation of foamy viruses from blood lymphocytes of infected humans is accelerated in a heterologous coculture system. Antibodies against gamma interferon (IFN-γ) are able to suppress most of the inhibitory activity, suggesting that IFN-γ is the dominant component.

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