Development of a lymphocyte-transformation-assay for peripheral blood lymphocytes of the harbor porpoise and detection of cytokines using the reverse-transcription polymerase chain reaction

2004 ◽  
Vol 98 (1-2) ◽  
pp. 59-68 ◽  
Author(s):  
A Beineke ◽  
U Siebert ◽  
N van Elk ◽  
W Baumgärtner
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Peripheral Blood ◽  
Lymphocyte Transformation ◽  
Peripheral Blood Lymphocytes ◽  
Harbor Porpoise ◽  
Chain Reaction ◽  
Blood Lymphocytes ◽  
Transformation Assay ◽  
Polymerase Chain

Failure to Detect Measles Virus Rna, by Reverse Transcription-Polymerase Chain Reaction, in Peripheral Blood Leucocytes of Patients with Multiple Sclerosis

1996 ◽  
Vol 1 (4) ◽  
pp. 204-206 ◽  
Author(s):  
B. Brankin ◽  
M. Osman ◽  
L. Herlihy ◽  
S.A. Hawkins ◽  
S.L. Cosby
Keyword(s):  
Multiple Sclerosis ◽  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Peripheral Blood ◽  
Measles Virus ◽  
Chain Reaction ◽  
Specific Primers ◽  
Polymerase Chain ◽  
Multiple Sclerosis Patients ◽  
Peripheral Blood Leucocytes

We have examined peripheral blood leucocytes (PBLs) from 17 multiple sclerosis patients, two patients with rheumatoid arthritis, one case of acute childhood measles and one case of subacute sclerosing panencephalitis, as well as 19 healthy adult controls for measles virus (MV) RNA, by the technique of reverse transcription-polymerase chain reaction. MV nucleocapsid gene specific primers were used to amplify all PBL-derived cDNA samples. These proved to be negative with the exception of the sample derived from the acute measles case. Selected cases were examined further, using fusion gene and matrix gene specific primers. MV RNA could not be detected.

scholarly journals The detection of melanoma cells in peripheral blood by reverse transcription-polymerase chain reaction

1995 ◽  
Vol 72 (1) ◽  
pp. 155-159 ◽  
Author(s):  
AJE Foss ◽  
MJ Guille ◽  
NL Occleston ◽  
PG Hykin ◽  
JL Hungerford ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Peripheral Blood ◽  
Melanoma Cells ◽  
Chain Reaction ◽  
Polymerase Chain
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