We have analyzed impact of polymorphism in GSTT1 and GSTM1 genes on the
micronuclei (MN) frequency in peripheral blood lymphocytes (PBLs). A total
134 women from central Serbia were enrolled in the study. Polymorphisms of
GST genes were genotyped by performing a multiplex polymerase chain reaction
(PCR) and cytokinesis block micronucleus (CBMN) test was used to assess MN
frequency. GSTT1 null and GSTM1 null genotype carriers had higher MN
frequencies as compared to positive counterparts but without statistical
significance. Carriers of dual GSTT1/GSTM1 null genotypes had significantly
higher MN frequency than positive/positive, positive/null and null/positive.
Smokers and women >45 years old with GSTT1 null genotype and
GSTT1null/GSTM1null genotypes have statistically higher MN frequency than
positive counterparts. Results suggest possible influence of dual null
genotypes of GSTT1/GSTM1 on the baseline MN frequency, as well influence on
the level of MN in smokers and in women age >45 years. GSTT1 null genotype
may have the potential to influence the baseline MN frequency in PBLs of
smokers, as well as in women age >45 years.
Detection of feline immunodeficiency proviral DNA in peripheral blood lymphocytes by the polymerase chain reaction
