Three-dimensional reconstruction from thin sections of crystals of ribosomal subunits

1982 ◽  
Vol 8 (4) ◽  
pp. 443-445
Author(s):  
A. Yonath
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Ribosomal Subunits ◽  
Thin Sections ◽  
Dimensional Reconstruction

Three-dimensional reconstruction of rigor insect flight muscle from tilted thin sections

Nature ◽  
1984 ◽  
Vol 310 (5975) ◽  
pp. 285-291 ◽  
Author(s):  
Kenneth A. Taylor ◽  
Mary C. Reedy ◽  
Leonidas Córdova ◽  
Michael K. Reedy
Keyword(s):  
Flight Muscle ◽  
Insect Flight ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Insect Flight Muscle ◽  
Thin Sections ◽  
Dimensional Reconstruction

Challenges of three-dimensional reconstruction of ribonucleoprotein complexes from electron spectroscopic images - Reconstructing ribosomal RNA

1996 ◽  
Vol 54 ◽  
pp. 52-53
Author(s):  
D.R. Beniac ◽  
G.J. Czarnota ◽  
T.A. Bartlett ◽  
F.P. Ottensmeyer ◽  
G. Harauz
Keyword(s):  
Ribosomal Rna ◽  
Correlation Functions ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Mutual Correlation ◽  
Ribosomal Subunits ◽  
E Coli ◽  
Ribonucleoprotein Complexes ◽  
Dimensional Reconstruction

Transmission electron microscopy has been dominant in structural studies of the ribosome and its constituent ribonucleic acids and proteins. Ribosomal RNA (rRNA) has central importance in the architecture of this complex and in protein synthesis. Our work has entailed using electron spectroscopic imaging (ESI) to probe the tertiary structure of rRNA in situ in a prokaryote (Escherichia coli) and in a eukaryote (Thermomyces lanuginosus). ESI uses only electrons which have lost a specific amount of energy due to specific inner-shell ionisation interactions with the specimen to form an elemental map. In nucleoprotein complexes, a map of the phosphorus distribution represents primarily a projection of the phosphate backbone of the nucleic acid component. The visualisation of rRNA in situ in the intact ribosomal subunit by ESI was demonstrated almost a decade ago to be feasible. Our work on quantitative image analysis of ES images of E. coli and Th. lanuginosus ribosomal subunits has presented unique challenges and has resulted in new algorithmic developments generally applicable to such images. These innovations include a singular pretreatment procedure, the use of mutual correlation functions rather than cross correlation functions to reduce the effect of low spatial frequency components, and angular determination using iterative quaternion-assisted angular reconstitution to compute a three-dimensional reconstruction. These investigations have produced direct information regarding ribosomal rRNA localisation in the ribosomal subunits of E. coli and Th. lanuginosus, and the position of non-conserved sequences.

The interpretation of granitic textures from serial thin sectioning, image analysis and three-dimensional reconstruction

1995 ◽  
Vol 59 (395) ◽  
pp. 203-211 ◽  
Author(s):  
D. N. Bryon ◽  
M. P. Atherton ◽  
R. H. Hunter
Keyword(s):  
Image Analysis ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Mineral Phase ◽  
Thin Sections ◽  
Thin Sectioning ◽  
Potential Problems ◽  
Dimensional Reconstruction ◽  
Textural Development

AbstractTextural development of the felsic phases in two granodioritic rocks from the zoned Linga superunit of the Peruvian Coastal Batholith has been characterized using serial thin sectioning, image analysis and three-dimensional reconstruction. The study has shown how each mineral phase contributed to the texture during the formation and development of a contiguous crystal framework and during subsequent restriction, isolation and occlusion of the melt-filled porosity. The work highlights the important factors and potential problems in the use of serial thin sections and imaging in the analysis of complex polyphase rock textures.

scholarly journals Three-dimensional reconstruction of the Z disk of sectioned bee flight muscle.

1989 ◽  
Vol 108 (5) ◽  
pp. 1761-1774 ◽  
Author(s):  
N Q Cheng ◽  
J F Deatherage
Keyword(s):  
Central Region ◽  
Actin Filaments ◽  
Flight Muscle ◽  
Three Dimensional ◽  
Opposite Polarity ◽  
Dimensional Structure ◽  
Three Dimensional Reconstruction ◽  
Thin Sections ◽  
Dimensional Reconstruction ◽  
Cross Links

The three-dimensional structure of the central region of the Z disk of honeybee flight muscle has been determined to a resolution of 70 A by three-dimensional reconstruction from electron micrographs of tilted thin sections. The reconstructions show a complex assembly in which actin filaments terminate and are cross-linked together; a number of structural domains of this network are resolved in quantitative three-dimensional detail. The central region of the Z disk contains two sets of overlapping actin filaments of opposite polarity, which originate in the sarcomeres adjacent to the Z disk, and connections between these filaments. The filaments are deflected by the attachment of cross-links; spacing between filaments change by greater than 100 A during their passage through the Z disk. Each actin filament is linked by connecting structures to four filaments of opposite polarity and two filaments are of the same polarity. Four types of connecting density domain are observed in association with pairs of filaments of opposite polarity: C1, C2, C3, and C5. Two of these, C3 and C5, are associated with the ends of actin filaments. Another connection, C4, is associated with three filaments of the same polarity; C4 is threefold symmetric.

Three-Dimensional Reconstruction of Rabbit-DerivedPneumocystis cariniifrom Serial-Thin Sections II: Intermediate Precyst

1991 ◽  
Vol 38 (4) ◽  
pp. 407-411 ◽  
Author(s):  
FRANCOIS PALLUAULT ◽  
BRUNO PIETRZYK ◽  
EDUARDO DEI-CAS ◽  
CHRISTIAN SLOMIANNY ◽  
BENOIT SOULEZ ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Thin Sections ◽  
Dimensional Reconstruction

Views of Ribosome Tetramers and the Localization of Ribosomal Subunits

1982 ◽  
Vol 40 ◽  
pp. 160-161
Author(s):  
M. K. Lamvik ◽  
E. Zeltler
Keyword(s):  
Chick Embryo ◽  
Three Dimensional ◽  
Reference Image ◽  
Three Dimensional Reconstruction ◽  
Ribosomal Subunits ◽  
Ribosome Structure ◽  
Left Handed ◽  
Dimensional Reconstruction ◽  
Fixed Orientation ◽  
Substrate Film

Carey suggested that chick-embryo ribosome tetramers attach to the substrate film preferentially in one of the two possible orientations, making them particularly appropriate for use in the three-dimensional reconstruction of ribosome structure. We have stored and analyzed tetramer images digitally as described earlier. To test for a fixed orientation, many tetramer Images were compared to a reference image that contained an arbitrarily labelled "right-handed" view. Images that correlated well with the reference were averaged, giving a right-handed result (fig. 1). To search for a left-handed view, the reference image was "turned over” in the computer to artificially create a left-handed image. Repeating the procedure with the new reference gave a result that was mostly symmetric (fig. 2), not left-handed.

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