An improved method for the selection of bacterial cells at division

1964 ◽  
Vol 82 (3) ◽  
pp. 608-610 ◽  
Author(s):  
Charles E. Helmstetter ◽  
Donald J. Cummings
2020 ◽  
Vol 16 (6) ◽  
pp. 891-899 ◽  
Author(s):  
Wissam Zam

Probiotics are viable microorganisms widely used for their claimed beneficial effects on the host health. A wide number of researchers proved that the intake of probiotic bacteria has numerous health benefits which created a big market of probiotic foods worldwide. The biggest challenge in the development of these products is to maintain the viability of bacterial cells during the storage of the product as well as throughout the gastrointestinal tract transit after consumption, so that the claimed health benefits can be delivered to the consumer. Different approaches have been proposed for increasing the resistance of these sensitive microorganisms, including the selection of resistant strains, incorporation of micronutrients, and most recently the use of microencapsulation techniques. Microencapsulation has resulted in enhancing the viability of these microorganisms which allows its wide use in the food industry. In this review, the most common techniques used for microencapsulation of probiotics will be presented, as well as the most usual microcapsule shell materials.


Author(s):  
Xinshui Yu ◽  
Zhaohui Yang ◽  
Kunling Song ◽  
Tianxiang Yu ◽  
Bozhi Guo

The distribution and parameters of the random variables is an important part of conventional reliability analysis methods, such as Monte Carlo method, which should be known fist before using these methods, but it is often hard or impossible to obtain. Model-free sampling technique puts forward a method to get the distribution of the random variables, but the accuracy of the extended sample generated by it is not enough. This paper presented an improved model-free sampling technique, which is based on Bootstrap methods, to increase the accuracy of the extended sample and decrease the iteration times. In this improved model-free sampling technique, the method of the selection of initial sample points and the generation of iterative sample is improved. Meanwhile, a center distance criterion, which considers the local characteristics of the extended sample, is added to the generating criterion of dissimilarity measure. The effectiveness of this improved method is illustrated through some numerical examples.


2014 ◽  
Vol 602-605 ◽  
pp. 3177-3180
Author(s):  
Wei Ping Cui ◽  
Li Juan Du

In this paper, through comparison and analysis of various wavelet denoising methods, a new threshold function is constructed, and the selection of threshold is improved. Signal denoising simulation is made by the software MATLAB, the results show that the improved method is superior to the traditional method, and obtain a better denoising effect.


1971 ◽  
Vol 11 (01) ◽  
pp. 23-37 ◽  
Author(s):  
C. Petrauskas ◽  
P.M. Aagaard

Abstract An improved method is presented for selecting offshore structure design waves by extrapolating historical storm data to obtain extreme value statistics. The method permits flexibility in choice of distribution functions through use of computerized procedures, estimates extrapolated wave-height procedures, estimates extrapolated wave-height uncertainty due to small sample size, and includes criteria for judging whether or not given wave-height values can be represented by one or more of the distributions implemented in the method. The relevance of uncertainty to selection of design-wave heights is discussed and illustrated. Introduction The problem of selecting design-wave heights for offshore platforms has many facets, ranging from the development of oceanographic data to the selection of the prudent level of engineering risk for a particular installation. This paper deals only with part of the problem; it describes an improved method for using the small available amount of wave-height information to estimate the extreme value statistics and associated uncertainties for the large storm waves that have a very low probability of occurrence. probability of occurrence. Hindcast wave-height information for design-wave studies usually covers a period of historical record that is shorter than the return period selected for acceptable engineering risk. Return periods commonly used for selection design waves are 100 years or more, but good meteorological data, on Which the calculated wave heights are based, can rarely be obtained for periods covering more than 50 to 60 years. As a consequence, extrapolations to longer return periods are necessary. Present methods for making the extrapolation employ probablistic models through the use of special probability graph papers on which a family of distribution functions plot as straight lines. The wave heights are plotted vs their "plotting-position" return period, and a straight line fitted to the plotted data is extended beyond the data to estimate extreme wave heights for return periods of interest. The methods are described in periods of interest. The methods are described in numerous technical papers and books; Refs. 1 through 5 are examples. The shortcomings of the present commonly used methods are:the straight line drawn through the data is in most cases visually fit to the data, thus is subject to error; andno information is available on the uncertainty of the resulting extrapolation. These shortcomings have been discussed by many authors and many of their concepts influenced this study. The improved method presented in this paper offers:greater flexibility in the choice of distributions through computerized procedures,guidelines for picking the "best" distribution from several implemented in the method, andprocedures for estimating the uncertainty of procedures for estimating the uncertainty of extrapolated wave heights. CONDENSED CONCLUSIONS Procedures described in this paper for extrapolating hindcast storm-wave heights and estimating uncertainty intervals to the extrapolated values are recommended as aids in selecting the design-wave height. The results of the extrapolating procedure and related uncertainty considerations procedure and related uncertainty considerations are only aids to help the engineer assess the risks associated with his design. The actual selection of the design-wave height is a matter of engineering judgment. The choice is subjective and will vary according to the risk chosen for the design. Further consideration of ways to decrease the span of be uncertainty intervals is warranted. Increasing the number of years represented in the sample along with the number of storms is a direct way to decrease the span. In the areas of the world having poor weather records the sample size will be marginal for many years to come. SPEJ P. 23


2015 ◽  
Vol 81 (5-6) ◽  
pp. 194-209 ◽  
Author(s):  
Camille L. A. Hamula ◽  
Hanyong Peng ◽  
Zhixin Wang ◽  
Ashley M. Newbigging ◽  
Gregory J. Tyrrell ◽  
...  
Keyword(s):  

2001 ◽  
Vol 74 (5) ◽  
pp. 435-442 ◽  
Author(s):  
Tomohiro Yoshikawa ◽  
Fumi Nakanishi ◽  
Yuki Ogura ◽  
Daisuke Oi ◽  
Takeshi Omasa ◽  
...  

Author(s):  
А.А. Грибанькова ◽  
М.А. Агиевич ◽  
О.А. Евтуховская ◽  
В.В. Гурченко ◽  
С. Чжай ◽  
...  

В основе работы лежит исследование воздействия органического соединения (ОС) – гидрокоричной (3-фенилпропановой) кислоты на бактериальные клетки СРБ (сульфатредуцирующих бактерий) являющиеся стимуляторами коррозионных процессов. Подбор условий использования и количественная оценка эффективности ингибирующего (биоцидного) действия исследуемого ОС, которое может быть применено для защиты стали от коррозии в средах, содержащих сульфатредуцирующие бактерии. В ходе исследования было изучено влияние ОС на развитие сульфатредуцирующих бактерий и определены такие параметры, как изменение значения pH и окислительно-восстановительного потенциала образцов в среде, численности бактерий и содержания биогенного сероводорода в зависимости от концентрации используемого ОС. Сделаны основные выводы о ингибирующем коррозию и биоцидном действиях испытуемого органического соединения путем оценки скорости коррозии образцов стали и защитном эффекте в зависимости от выбранных концентраций органического соединения методом гравиметрии. Дополнительная оценка ингибирующего действия была проведена методом электрохимической импедансной спектроскопии и методом снятия поляризационных кривых испытуемых образцов. The study is based on the study of the effect of an organic compound (OC) – hydrocinnamic (3-phenylpropanoic) acid on the bacterial cells of SRB (sulfate-reducing bacteria), which are stimulants of corrosion processes. Selection of conditions of use and a quantitative assessment of the effectiveness of the inhibitory (biocidal) action of the studied OC, which can be used to protect steel from corrosion in environments containing SRB. In the course of the study, the effect of OC on the development of SRB was studied and such parameters as the change in the pH value and the redox potential of the samples in the medium, the number of bacteria and the content of biogenic hydrogen sulfide depending on the concentration of the used OC were determined. The main conclusions are drawn about the inhibitory corrosion and biocidal actions of the test OC by assessing the corrosion rate of steel samples and the protective effect depending on the selected concentrations of the OC by gravimetry. An additional assessment of the inhibitory effect was carried out by the method of electrochemical impedance spectroscopy and by the method of taking the polarization curves of the test samples.


Author(s):  
Trey Walters ◽  
Trygve Dahl ◽  
David C. Rogers

Abstract In some situations, it is possible for flow to go backwards through a pump during a transient waterhammer event. Sustained reverse flow will lead to reverse rotation. Understanding and predicting the pump behavior during waterhammer under these conditions is typically accomplished using previously published four quadrant pump data. Historically, the selection of which data to use is based on the similarity of pump specific speed. The weaknesses of using specific speed are described and an improved method of selecting appropriate four quadrant data is given based on fundamental curve shapes for head and power in the normal operating zone.


2004 ◽  
Vol 70 (1) ◽  
pp. 494-497 ◽  
Author(s):  
K. Zwirglmaier ◽  
W. Ludwig ◽  
K.-H. Schleifer

ABSTRACT We developed an improved method for cultivation-independent sorting of bacterial cells. The technique is based on labeling the target cells by in situ hybridization with polynucleotide transcript probes. Due to the probes' length, part of the probe remains outside the cell and can subsequently be used to capture the cells. Target cells are immobilized during a second hybridization step in microplates that are coated with DNA that is complementary to the probe sequence. The method was applied successfully to artificial mixtures of cells with polynucleotide probes targeting either rRNA, a plasmid-borne beta-lactamase gene, or a chromosome-borne glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. Cells could be separated based on phylogenetic parameters (using rRNA-targeted probes) as well as on other DNA-encoded traits.


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