Late spermatocytes from immature rat testis isolation, electron microscopy, lectin agglutinability and capacity for glycoprotein and sulfogalactoglycerolipid biosynthesis

1978 ◽  
Vol 541 (1) ◽  
pp. 59-75 ◽  
Author(s):  
Pamela J. Letts ◽  
Richard C. Hunt ◽  
Margaret A. Shirley ◽  
Les Pinteric ◽  
Harry Schachter
Keyword(s):  
Electron Microscopy ◽  
Immature Rat ◽  
Rat Testis

EFFECTS OF SHORT-TERM BILATERAL AND UNILATERAL CASTRATION AND ANDROGEN REPLACEMENT ON THE METABOLISM OF [3H]TESTOSTERONE IN VITRO BY THE EPIDIDYMIS OF THE IMMATURE RAT

1981 ◽  
Vol 91 (1) ◽  
pp. 53-60 ◽  
Author(s):  
R. G. FOLDESY ◽  
J. H. LEATHEM
Keyword(s):  
Normal Function ◽  
In Vitro Metabolism ◽  
Short Term ◽  
Immature Rat ◽  
Testosterone Treatment ◽  
Androgen Metabolism ◽  
Rat Testis ◽  
Androgen Withdrawal ◽  
Cauda Epididymidis

The in-vitro metabolism of [3H]testosterone by the epididymis of the pubertal rat (55 days of age) has been examined after short-term bilateral and unilateral castration and androgen replacement. Bilateral castration did not decrease the metabolism of [3H]testosterone, but did result in a decline in the proportion of 5α-dihydrotestosterone produced and an increase in that of 5α-androstane-3α,17β-diol, androsterone and 5α-androstane-3,17-dione. Changes in metabolism occurred in the caput within 2 days after surgery, but not until 5 days after surgery in the cauda epididymidis. Daily testosterone treatment, which maintained prostatic growth in bilaterally castrated animals, did not restore normal androgen metabolism and increased further the production of androsterone and 5α-androstanedione by the cauda. In unilaterally castrated animals, androgen metabolism in epididymal tissue from the operated side was normal in the cauda but was indistinguishable in the caput from that of bilaterally castrated rats. These results indicate that (a) androgen metabolism by the caput, compared to that by the cauda, responds more quickly to androgen withdrawal and (b) that in the short term, normal androgen metabolism by the caput, but not the cauda, is dependent upon the presence of the ipsilateral testis. Furthermore, testosterone alone proved an inadequate replacement for bilateral castration which implies that the pubertal rat testis secretes additional compounds which are essential for normal function of the epididymis.

scholarly journals Doxorubicin Induces Apoptosis in Germ Line Stem Cells in the Immature Rat Testis and Amifostine Cannot Protect against This Cytotoxicity

2005 ◽  
Vol 65 (21) ◽  
pp. 9999-10005 ◽  
Author(s):  
Mi Hou ◽  
Dionisios Chrysis ◽  
Mirja Nurmio ◽  
Martti Parvinen ◽  
Staffan Eksborg ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Line ◽  
Immature Rat ◽  
Rat Testis

scholarly journals Biochemical characterization of integral membrane heparan sulfate proteoglycans in Sertoli cells from immature rat testis

2001 ◽  
Vol 1510 (1-2) ◽  
pp. 474-487 ◽  
Author(s):  
Sylvie Brucato ◽  
Gaëlle Fagnen ◽  
Corinne Villers ◽  
Pierre-Jacques Bonnamy ◽  
Monique Langris ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Sertoli Cells ◽  
Biochemical Characterization ◽  
Heparan Sulfate Proteoglycans ◽  
Immature Rat ◽  
Rat Testis

Cathodoluminescence-emitting lipid droplets in rat testis: a study by analytical color fluorescence electron microscopy

1993 ◽  
Vol 271 (2) ◽  
pp. 217-225 ◽  
Author(s):  
Gang Ning ◽  
Toyoshi Fujimoto ◽  
Hirotami Koike ◽  
Kazuo Ogawa
Keyword(s):  
Electron Microscopy ◽  
Lipid Droplets ◽  
Rat Testis

Steroid Metabolism by the Immature Rat Testis

1974 ◽  
Vol 52 (9) ◽  
pp. 744-750 ◽  
Author(s):  
W. H. Moger ◽  
D. T. Armstrong
Keyword(s):  
Luteinizing Hormone ◽  
Male Rats ◽  
Steroid Metabolism ◽  
Immature Rat ◽  
Rat Testis ◽  
Immature Male ◽  
Immature Rats ◽  
Rapid Conversion

Treatment of hypophysectomized immature male rats with luteinizing hormone (LH) greatly increased the metabolism of both 4-[14C]progesterone and 4-[14C]testosterone by testicular homogenates. Prolactin, either alone or in combination with LH, did not influence the metabolism of either substrate. Progesterone was metabolized to 17α-hydroxyprogesterone, androstenedione, 5α-pregnan-3,20-dione, 3α-hydroxy-5α-pregnan-20-one, and 3β-hydroxy-5α-pregnan-20-one. Testosterone was metabolized to dihydrotestosterone and 5α-androstan-3α,17β-diol. On the basis of these observations it is suggested that LH stimulated the 5α-reductase(s) of the immature rat testis. Testis homogenates from immature rats with intact pituitaries were incubated with 4-[14C]3α-hydroxy-5α-pregnan-20-one. Rapid conversion to androsterone was observed, with the formation of a compound chromatographically identical with 3α, 17α-dihydroxy-5α-pregnan-20-one as an apparent intermediate. These findings demonstrate the ability of the rat testes to form androsterone from progesterone by a pathway that does not involve testosterone.

scholarly journals IGF-1 stimulates synthesis of undersulfated proteoglycans and of hyaluronic acid by peritubular cells from immature rat testis

1997 ◽  
Vol 1358 (2) ◽  
pp. 127-141 ◽  
Author(s):  
Bénédicte Thiébot ◽  
Lahcen Bichoualne ◽  
Monique Langris ◽  
Pierre-Jacques Bonnamy ◽  
Pierre Barbey ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Immature Rat ◽  
Rat Testis ◽  
Peritubular Cells

Correlative light and electron microscopy of dissociated immature rat testicular cells undergoing morphogenesis in vitro

Development ◽  
1980 ◽  
Vol 60 (1) ◽  
pp. 283-293
Author(s):  
L. A. Erickson ◽  
J. C. Davis ◽  
P. R. Burton ◽  
J. Snyder
Keyword(s):  
Electron Microscopy ◽  
Primary Culture ◽  
Cell Monolayer ◽  
Light And Electron Microscopy ◽  
Outer Cell ◽  
Culture Dish ◽  
Tubular Aggregates ◽  
Immature Rat ◽  
Testicular Cells

Immature rat testicular cells undergo morphogenesis in primary culture (Davis, 1978). Depending upon the number of dissociated testicular cells added to the culture dish, spherical or tubular aggregates were formed. Spherical aggregates resulted from movement of cells into centers of aggregation and the detachment of these cells from the substratum; on the other hand, tubular aggregates resulted from detachment and retraction of the cell monolayer at certain points along its outer edge. In this investigation, the different methods of formation of aggregates by immature rat testicular cells in primary culture were examined with the scanning electron microscope (SEM). The cell types involved in such morphogenesis and their associations within completely formed structures were examined by transmission electron microscopy (TEM). In addition, the rates of formation of aggregates were established by time-lapse cinemicroscopy. During formation of spherical aggregates, the rate of recruitment of cells into centers of aggregation (0·04± 0·006 μm/min; x±S.E.M., n = 78) was much slower than the rate of cell detachment during formation of tubular aggregates (11·7 ± 1·8 μm/sec; x±S.E.M., n = 110). Although specific roles for each cell type in formation of aggregates have not been determined, the associations of cells within the two types of reformed aggregates appeared to be similar. Myofibroblast cells were located in outer cell layers and Sertoli cells were observed to underlie the layers of myofibroblasts in both types of aggregates. Germinal cells, however, were found on the outer surface of spherical aggregates, but in tubular aggregates they were located on the inner surface. Since spherical and tubular aggregates are formed by different methods, this observation suggests that rearrangement of cells within the aggregates takes place and contributes to the internal morphology of newly-formed aggregates.

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