Utilization of aminolevulinic acid as a nitrogen source by a green alga

1985 ◽  
Vol 37 (3) ◽  
pp. 213-216 ◽  
Author(s):  
Richard Ellis ◽  
Mark Greenawald
2019 ◽  
Vol 9 (3) ◽  
pp. 1070-1082 ◽  
Author(s):  
Sabrina C. Lachmann ◽  
Tabea Mettler‐Altmann ◽  
Alexander Wacker ◽  
Elly Spijkerman
Keyword(s):  

1985 ◽  
Vol 63 (5) ◽  
pp. 909-915 ◽  
Author(s):  
Kazuo Nakamura ◽  
Charles F. Landry ◽  
Christine A. Goertzen ◽  
N. Wayne Ikebuchi

To address the problem of amino acid auxotroph scarcities in algae, an explanation was sought specifically for the nonrecoverability of arginine auxotrophs in the unicellular green alga Chlamydomonas eugametos. Chlamydomonas reinhardtii, in which the auxotroph has been recovered, was taken as a reference. In C. eugametos, unlike previously reported in C. reinhardtii, the use of selective media free of [Formula: see text] appeared not to affect the mutation spectrum. Arginine supported growth as the sole nitrogen source and canavanine sulfate inhibited growth, but both effects were less pronounced in C. eugametos. The concentration ratios (based on intracellular arginine accumulation) in C. eugametos remained less than 23% that of C. reinhardtii. The results indicate that nonrecoverability of arginine auxotrophs in C. eugametos is due to insufficient cellular accessibility to arginine.


Author(s):  
L. P. Hardie ◽  
D. L. Balkwill ◽  
S. E. Stevens

Agmenellum quadruplicatum is a unicellular, non-nitrogen-fixing, marine cyanobacterium (blue-green alga). The ultrastructure of this organism, when grown in the laboratory with all necessary nutrients, has been characterized thoroughly. In contrast, little is known of its ultrastructure in the specific nutrient-limiting conditions typical of its natural habitat. Iron is one of the nutrients likely to limit this organism in such natural environments. It is also of great importance metabolically, being required for both photosynthesis and assimilation of nitrate. The purpose of this study was to assess the effects (if any) of iron limitation on the ultrastructure of A. quadruplicatum. It was part of a broader endeavor to elucidate the ultrastructure of cyanobacteria in natural systemsActively growing cells were placed in a growth medium containing 1% of its usual iron. The cultures were then sampled periodically for 10 days and prepared for thin sectioning TEM to assess the effects of iron limitation.


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