Peroxidase assay using 3,3′,5,5′ tetramethyl benzidine as H-donor for rapid diagnosis of iron deficiency in citrus

1990 ◽  
Vol 42 (3) ◽  
pp. 251-255 ◽  
Author(s):  
A.L. García ◽  
L. Galindo ◽  
M.J. Sánchez-Blanco ◽  
A. Torrecillas
Keyword(s):  
Iron Deficiency ◽  
Rapid Diagnosis ◽  
Tetramethyl Benzidine

Rapid Diagnosis of Iron Deficiency by Measurement of Free Erythrocyte Porphyrins and Hemoglobin: The FEP/Hemoglobin Ratio

PEDIATRICS ◽  
1976 ◽  
Vol 57 (1) ◽  
pp. 136-141
Author(s):  
Sergio Piomelli ◽  
Anthony Brickman ◽  
Edwina Carlos
Keyword(s):  
Iron Deficiency ◽  
Filter Paper ◽  
Transferrin Saturation ◽  
Rapid Diagnosis ◽  
Hemoglobin Level ◽  
Deficiency Anemia ◽  
Small Children ◽  
Blood Samples ◽  
Populations At Risk ◽  
Thalassemia Trait

The concentration of free erythrocyte porphyrins (FEP) and of hemoglobin can be measured on blood samples spotted on filter paper. The FEP/hemoglobin ratio in iron deficiency increases exponentially with a decrease of both transferrin saturation and hemoglobin level. The FEP/hemoglobin is an indicator of impaired heme synthesis. In small children an elevation of the FEP/hemoglobin ratio is a better indicator of iron-deficiency anemia than low transferrin saturation. The FEP/hemoglobin ratio is normal in thalassemia trait and renal anemia but it may be elevated in sickle-cell anemia. Measurement of FEP and hemoglobin on filter paper provides a useful diagnostic tool for the diagnosis of iron deficiency, of anemia, and (in populations at risk) of lead intoxication.

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

Is iron deficiency investigated appropriately?

2001 ◽  
Vol 120 (5) ◽  
pp. A728-A729
Author(s):  
A DIMAMBRO ◽  
T BROOKLYN ◽  
N HASLAM
Keyword(s):  
Iron Deficiency

Letter: Iron deficiency and thrombocytosis

1974 ◽  
Vol 133 (6) ◽  
pp. 1070-1071 ◽  
Author(s):  
K. Dincol
Keyword(s):  
Iron Deficiency
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