Mapping of the canine lumbosacral spinal cord neurons by nauta method at the end of the early phase of paraplegia induced by ischemia and reperfusion

Neuroscience ◽  
1991 ◽  
Vol 45 (2) ◽  
pp. 479-494 ◽  
Author(s):  
J. Marsala ◽  
I. Sulla ◽  
M. Santa ◽  
M. Marsala ◽  
L. Zacharias ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Early Phase ◽  
Ischemia And Reperfusion ◽  
Spinal Cord Neurons ◽  
Lumbosacral Spinal Cord ◽  
Nauta Method

scholarly journals Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method

2004 ◽  
Vol 47 (3) ◽  
pp. 209-211
Author(s):  
Eva Mechírová ◽  
Iveta Domoráková ◽  
Marianna Feriková
Keyword(s):  
Spinal Cord ◽  
Protein Aggregation ◽  
Neuronal Death ◽  
Ischemia Reperfusion ◽  
Spinal Cord Ischemia ◽  
Ischemia And Reperfusion ◽  
Injured Spinal Cord ◽  
Spinal Cord Neurons ◽  
Lumbosacral Spinal Cord ◽  
Nauta Method

Using ubiquitin immunohistochemistry and impregnative Nauta method we demonstrated that ubiquitin positivity and Nauta positivity in the neurons affected with ischemic injury in the lumbosacral spinal cord of rabbits and dogs may be of the same origin. Increased number of ubiquitin-positive aggregates was found in the cytoplasm of neurons in the intermediate zone and lamina IX of ventral horns of spinal cord in rabbits after 30 min of ischemia followed by 24 h lasting reperfusion. Nauta-positive, flocculent, intracytoplasmic, dark clusters appeared in the same localization in the canine lumbosacral spinal cord neurons after 30 min of ischemia and 24 h of reperfusion. Ubiquitin aggregates and Nauta-positive dark clusters in the injured spinal cord neurons could be the first light microscopic signs of slow neuronal death following spinal cord ischemia and reperfusion.

Increased expression of spinal cord Fos protein induced by bladder stimulation after spinal cord injury

2000 ◽  
Vol 279 (1) ◽  
pp. R295-R305 ◽  
Author(s):  
Margaret A. Vizzard
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Afferent Pathways ◽  
Spinal Cord Neurons ◽  
Bladder Distension ◽  
Lumbosacral Spinal Cord ◽  
Fos Protein ◽  
Cord Injury ◽  
Bladder Afferents ◽  
Fos Expression

These studies examined Fos protein expression in spinal cord neurons synaptically activated by stimulation of bladder afferent pathways after spinal cord injury (SCI). In urethan-anesthetized Wistar rats after SCI for 6 wk, intravesical saline distension significantly ( P ≤ 0.005) increased the number of Fos-immunoreactive (IR) cells in the rostrolumbar (L1, 38 cells/section; L2, 29 cells/section) and caudal lumbosacral (L6, 140 cells/section; S1, 110 cells/section) spinal cord compared with control animals, but Fos expression in the L5 segment was not altered. The distribution of Fos-IR cells was also altered in the lumbosacral spinal cord. Significantly greater numbers of Fos-IR cells were distributed in the dorsal commissure and medial and lateral dorsal horn after intravesical distension in SCI animals. Large percentages of parasympathetic (75%) and sympathetic (85%) preganglionic neurons also expressed Fos-IR after intravesical distension in SCI animals. These results demonstrate that bladder distension produces increased numbers and an altered distribution pattern of Fos-IR cells after SCI. This pattern resembles that after noxious irritation of the bladder in control animals. Pretreatment with capsaicin significantly reduced the number of Fos-IR cells induced by bladder distension after SCI. These data suggest that SCI can reveal an altered Fos expression pattern in response to a nonnoxious bladder stimulus that is partially mediated by capsaicin-sensitive bladder afferents.

scholarly journals Kv3 channels contribute to the excitability of sub-populations of spinal cord neurons in lamina VII

2021 ◽  
Author(s):  
Pierce Mullen ◽  
Nadia Pilati ◽  
Charles H Large ◽  
Jim Deuchars ◽  
Susan A Deuchars
Keyword(s):  
Spinal Cord ◽  
Firing Frequency ◽  
Neuronal Firing ◽  
Recurrent Inhibition ◽  
Inhibitory Neurons ◽  
Spinal Cord Neurons ◽  
Lumbosacral Spinal Cord ◽  
Whole Cell Patch Clamp ◽  
Neuronal Types ◽  
Fast Firing

Autonomic parasympathetic preganglionic neurons (PGN) drive contraction of the bladder during micturition but remain quiescent during bladder filling. This quiescence is postulated to be due to recurrent inhibition of PGN by fast-firing adjoining interneurons. Here, we defined four distinct neuronal types within lamina VII of the lumbosacral spinal cord, where PGN are situated, by combining whole cell patch clamp recordings with k-means clustering of a range of electrophysiological parameters. Additional morphological analysis separated these neuronal classes into parasympathetic preganglionic populations (PGN) and a fast firing interneuronal population. Kv3 channels are voltage-gated potassium channels (Kv) that allow fast and precise firing of neurons. We found that blockade of Kv3 channels by tetraethylammonium (TEA) reduced neuronal firing frequency and isolated high-voltage-activated Kv currents in the fast-firing population but had no effect in PGN populations. Furthermore, Kv3 blockade potentiated the local and descending inhibitory inputs to PGN indicating that Kv3-expressing inhibitory neurons are synaptically connected to PGN. Taken together, our data reveal that Kv3 channels are crucial for fast and regulated neuronal output of a defined population that may be involved in intrinsic spinal bladder circuits that underpin recurrent inhibition of PGN.

scholarly journals Ubiquitin Activity Following Ischemia and Reperfusion of Spinal Cord Neurons in the Rabbit

2004 ◽  
Vol 73 (1) ◽  
pp. 51-54 ◽  
Author(s):  
M. Feriková ◽  
E. Mechírová ◽  
I. Domoráková
Keyword(s):  
Spinal Cord ◽  
Ischemia And Reperfusion ◽  
Spinal Cord Neurons

scholarly journals Rostrocaudal Distribution of the C-Fos-Immunopositive Spinal Network Defined by Muscle Activity during Locomotion

2021 ◽  
Vol 11 (1) ◽  
pp. 69
Author(s):  
Natalia Merkulyeva ◽  
Vsevolod Lyakhovetskii ◽  
Aleksandr Veshchitskii ◽  
Oleg Gorskii ◽  
Pavel Musienko
Keyword(s):  
Spinal Cord ◽  
Locomotor Activity ◽  
Control Systems ◽  
Muscle Activity ◽  
Knee Extensors ◽  
Emg Activity ◽  
Lumbosacral Spinal Cord ◽  
Adductor Muscles ◽  
Spinal Network ◽  
Hip Flexor

The optimization of multisystem neurorehabilitation protocols including electrical spinal cord stimulation and multi-directional tasks training require understanding of underlying circuits mechanisms and distribution of the neuronal network over the spinal cord. In this study we compared the locomotor activity during forward and backward stepping in eighteen adult decerebrated cats. Interneuronal spinal networks responsible for forward and backward stepping were visualized using the C-Fos technique. A bi-modal rostrocaudal distribution of C-Fos-immunopositive neurons over the lumbosacral spinal cord (peaks in the L4/L5 and L6/S1 segments) was revealed. These patterns were compared with motoneuronal pools using Vanderhorst and Holstege scheme; the location of the first peak was correspondent to the motoneurons of the hip flexors and knee extensors, an inter-peak drop was presumably attributed to the motoneurons controlling the adductor muscles. Both were better expressed in cats stepping forward and in parallel, electromyographic (EMG) activity of the hip flexor and knee extensors was higher, while EMG activity of the adductor was lower, during this locomotor mode. On the basis of the present data, which showed greater activity of the adductor muscles and the attributed interneuronal spinal network during backward stepping and according with data about greater demands on postural control systems during backward locomotion, we suppose that the locomotor networks for movements in opposite directions are at least partially different.

Expression of GAD mRNA in spinal cord neurons of normal and monoarthritic rats

1994 ◽  
Vol 26 (1-2) ◽  
pp. 169-176 ◽  
Author(s):  
J.M. Castro-Lopes ◽  
T.R. Tölle ◽  
B. Pan ◽  
W. Zieglgänsberger
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Neurons
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