Isolation and purification of superoxide dismutase purified from Brussels sprouts (Brassica oleracea L. var. bullata sub var. gemmifera)

1991 ◽  
Vol 41 (1) ◽  
pp. 1-9 ◽  
Author(s):  
J.L. Walker ◽  
K.M. McLellan ◽  
D.S. Robinson
Keyword(s):  
Superoxide Dismutase ◽  
Brassica Oleracea ◽  
Brussels Sprouts ◽  
Isolation And Purification ◽  
Brassica Oleracea L

scholarly journals Antioxidant Enzyme Activities of some Brassica Species

2017 ◽  
Vol 74 (2) ◽  
pp. 191
Author(s):  
Rodica SOARE ◽  
Maria DINU ◽  
Cristina BĂBEANU ◽  
Mihaela POPESCU
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Enzymatic Activity ◽  
Brassica Oleracea ◽  
Radical Cation ◽  
Colorimetric Method ◽  
Antioxidant Enzyme Activities ◽  
Red Cabbage ◽  
Soluble Peroxidase ◽  
Brassica Oleracea L

This paper set out to comparatively study five species: white cabbage (Brassica oleracea L. var. capitata alba Alef.), red cabbage (Brassica oleracea L. var. capitata f. rubra Alef.), Kale (Brassica oleracea L. var. Acephala), cauliflower (Brassica oleracea var. botrytis) and broccoli (Brassica oleracea var. cymosa) in order to identify those with high enzymatic and antioxidant activities. The enzymatic activity of superoxide dismutase (SOD), catalase (CAT) and soluble peroxidase (POX) as well as the antioxidant activity against 2.2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation were determined. Total superoxide dismutase activity was measured spectrophotometrically based on inhibition in the photochemical reduction of nitroblue tetrazolium. Total soluble peroxidase was assayed by measuring the increase in A436 due to the guaiacol oxidation and the catalase activity was assayed through the colorimetric method. The capacity of extracts to scavenge the ABTS radical cation was assessed colorimetric using Trolox as a standard. The obtained results show that studied enzymatic activities and the antioxidant activity against ABTS vary depending on the analyzed species. So, among the studied Brassicaceae species, it emphasize red cabbage with the highest enzymatic activity (CAT 22.54 mM H2O2/min/g and POX 187.2 mM ΔA/1min/1g f.w.) and kale with highest antioxidant activity, of 767 μmol TE/100g f.w. The results of this study recommendintroducing the studied varieties in diet due to the rich antioxidant properties.

The effect of aerated hydration on DNA synthesis in embryos of Brassica oleracea L.

1993 ◽  
Vol 3 (3) ◽  
pp. 195-199 ◽  
Author(s):  
J. M. Thornton ◽  
A. R. S. Collins ◽  
A. A. Powell
Keyword(s):  
Dna Damage ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Brassica Oleracea ◽  
Thymidine Incorporation ◽  
Recovery Process ◽  
Time Difference ◽  
Brussels Sprouts ◽  
Brassica Oleracea L

AbstractAgeing causes a delay in the onset of DNA replication in seeds. Aerated hydration for 8 h, a treatment to invigorate seeds, resulted in a reduction of about 12 h in the time difference in the onset of DNA synthesis between unaged and aged embryos of brassica seed. This effect is consistent with a recovery process occurring during aerated hydration of the seed involving the repair of accumulated DNA damage. The occurrence of hydroxyurea-resistant 3H-thymidine incorporation in aged Brussels sprouts embryos during the period 16–32 h from the start of hydration supports this interpretation.

Microspore embryogenesis and plant regeneration in Brussels sprouts (Brassica oleracea L. var. gemmifera)

2015 ◽  
Vol 191 ◽  
pp. 31-37 ◽  
Author(s):  
Aisong Zeng ◽  
Yuanyuan Yan ◽  
Jiyong Yan ◽  
Lixiao Song ◽  
Bing Gao ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Brassica Oleracea ◽  
Microspore Embryogenesis ◽  
Brussels Sprouts ◽  
Brassica Oleracea L

Cole crop yield response to reduced nitrogen rates

1999 ◽  
Vol 79 (1) ◽  
pp. 149-151 ◽  
Author(s):  
K. R. Sanderson ◽  
J. A. Ivany
Keyword(s):  
Brassica Oleracea ◽  
Yield Response ◽  
Marketable Yield ◽  
Brussels Sprouts ◽  
Control Banding ◽  
Reduced Nitrogen ◽  
N Rates ◽  
Nitrogen Rates ◽  
Cole Crop ◽  
Brassica Oleracea L

Response of cabbage (Brassica oleracea L. var. capitata), Brussels sprouts (Brassica oleracea var. gemmifera) and broccoli (Brassica oleracea L. var italica) to reduced N rates was studied over 4 yr. Treatments were application methods, broadcast, split and banded with two rates of N; 120 and 90 kg ha−1 plus a control of 150 kg ha−1 broadcast N. Marketable yield was lower by 15, 23, and 13%, respectively, compared to the control. Banding lowered yield of cabbage and broccoli. The lower N rate had the lowest yield in all crops. Leaf N concentration varied with N rate. Key words: Broccoli, Brussels sprouts, cabbage, reduced nitrogen, yield

BORON NUTRITION OF BROCCOLI, BRUSSELS SPROUTS, AND CAULIFLOWER GROWN ON PRINCE EDWARD ISLAND SOILS

1973 ◽  
Vol 53 (3) ◽  
pp. 275-279 ◽  
Author(s):  
UMESH C. GUPTA ◽  
J. A. CUTCLIFFE
Keyword(s):  
Brassica Oleracea ◽  
Prince Edward Island ◽  
Growing Season ◽  
Water Soluble ◽  
Hot Water ◽  
Optimum Growth ◽  
Brussels Sprouts ◽  
Deficiency Symptoms ◽  
Brassica Oleracea L

Broccoli (Brassica oleracea L. ssp. italica Plenck., cult Waltham 29), Brussels sprouts (Brassica oleracea L. ssp. gemmifera, cult Jade Cross), and cauliflower (Brassica oleracea L. ssp. botrytis, cult Snowball Y) were grown at four locations on Prince Edward Island with five levels of applied B at each location. The levels of applied B were: 0, 0.56, 1.12, 2.24, and 4.48 kg B/ha. No B deficiency symptoms, with the exception of hollow stem in cauliflower and broccoli, were noted. The incidence of hollow stem in these crops was not affected by applied B. The B concentration of tissues of all three crops was generally lower late in the growing season than earlier. Boron levels in the first tissue samplings were 13–70, 13–101, and 8–97 ppm in broccoli, Brussels sprouts, and cauliflower, respectively, and were found to be in the sufficiency range. A hot-water-soluble B content of 0.34–0.49 ppm in soil was sufficient for optimum growth of the crops. No B toxicity was observed even when the rate of applied B was 4.48 kg/ha.

TOLERANCE OF BRUSSELS SPROUTS TO HIGH BORON LEVELS

1987 ◽  
Vol 67 (1) ◽  
pp. 205-207 ◽  
Author(s):  
UMESH C. GUPTA ◽  
J. A. CUTCLIFFE ◽  
RENALD CORMIER
Keyword(s):  
Key Words ◽  
Brassica Oleracea ◽  
Leaf Tissue ◽  
Field Studies ◽  
Eastern Canada ◽  
Brussels Sprouts ◽  
Application Rates ◽  
High Boron ◽  
Brassica Oleracea L

Boron application rates as high as 8 and 16 kg ha−1 were not toxic to Brussels sprouts (Brassica oleracea L. ssp. gemmifera). These rates resulted in leaf tissue B levels of 123 and 161 μg g−1 and of 7.6 and 9.1 μg HWS B g−1 soil, respectively. Rates of 8–16 kg B ha−1 would, however, be seriously toxic to many crops. It is therefore strongly recommended that rates of B applied to Brussels sprouts should not exceed 2 kg ha−1. Such B applications should eliminate B deficiency in most crops and would not pose a threat of B toxicity to crops grown in the following year. Key words: Eastern Canada, Brussels sprouts yields, field studies, boron

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