Purification and characterization of glycerol phosphate dehydrogenase produced from wheat bran using a mixed culture system

1994 ◽  
Vol 51 (1) ◽  
pp. 33-37
Author(s):  
Z OLAMA ◽  
M ELSAYED ◽  
S TEMSAH ◽  
A ELBESOUMY ◽  
M HAMZA
Keyword(s):  
Mixed Culture ◽  
Wheat Bran ◽  
Culture System ◽  
Glycerol Phosphate ◽  
Purification And Characterization ◽  
Glycerol Phosphate Dehydrogenase

Purification and characterization of Mycobacterium tuberculosis indole-3-glycerol phosphate synthase

2006 ◽  
Vol 71 (S1) ◽  
pp. S38-S43 ◽  
Author(s):  
Yanping Yang ◽  
Min Zhang ◽  
Hongmei Zhang ◽  
Jianqiang Lei ◽  
Ruiliang Jin ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Glycerol Phosphate ◽  
Purification And Characterization ◽  
Phosphate Synthase

Isolation, purification and characterization of a complex heteroxylan from industrial wheat bran

1982 ◽  
Vol 30 (3) ◽  
pp. 488-495 ◽  
Author(s):  
Jean Marc Brillouet ◽  
Jean Paul Joseleau ◽  
Jean Pierre Utille ◽  
Dominique Lelievre
Keyword(s):  
Wheat Bran ◽  
Purification And Characterization

Purification and characterization of chitinase from wheat bran

2008 ◽  
Vol 136 ◽  
pp. S303
Author(s):  
Wan-Taek Ju ◽  
Zhao Yong ◽  
Gyung-Hyun Jo ◽  
Woo-Jin Jung ◽  
Ro-Dong Park
Keyword(s):  
Wheat Bran ◽  
Purification And Characterization

Purification and Characterization of Ginsenoside-Hydrolyzing β-Glucosidase from Wheat Bran

2013 ◽  
Vol 641-642 ◽  
pp. 906-909
Author(s):  
Chun Zhi Zhang ◽  
Ming Chen ◽  
Hai Chen Guo ◽  
Guo Ren Zu ◽  
Li Chen
Keyword(s):  
Molecular Weight ◽  
Wheat Bran ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Deae Cellulose ◽  
Polyacrylamide Gel ◽  
Purification And Characterization ◽  
Enzyme Solution ◽  
Crude Enzyme Solution

The ginsenoside-hydrolyzing β-glucosidase that can converse the major ginsenosides into the minor ginsenosides was isolated from wheat bran, and the enzyme was purified and characterized. The crude enzyme solution extracted from wheat bran could hydrolyse the protopanaxadiol-type ginsenosides such as Rb1, Rc, Rd and Rg3, but could not hydrolyse the protopanaxatriol-type ginsenosides such as Re and Rg2. The enzyme fractionated on the DEAE-Cellulose DE-52 column was purified to one spot in SDS polyacrylamide gel electrophoresis, and the molecular weight of enzyme in the fraction 34, 47, and 61 was approximately 62 kDa, 62 kDa, and 68 kDa, respectively.

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