RNA metabolism in isolated nuclei: Effect of temperature on RNA transport from intact and membrane-denuded nuclei

1981 ◽  
Vol 5 (1) ◽  
pp. 27-36 ◽  
Author(s):  
R PATTERSON ◽  
M LYERLY ◽  
S STUART
Keyword(s):  
Rna Metabolism ◽  
Effect Of Temperature ◽  
Rna Transport ◽  
Isolated Nuclei

scholarly journals Nature of the facilitated messenger ribonucleic acid transport from isolated nuclei

1976 ◽  
Vol 154 (2) ◽  
pp. 379-385 ◽  
Author(s):  
A Yannarell ◽  
D E. Schumm ◽  
T E Webb
Keyword(s):  
Concentration Dependence ◽  
Ribonucleic Acid ◽  
High Specificity ◽  
Effective Concentration ◽  
Regulatory Proteins ◽  
Rna Transport ◽  
Acid Transport ◽  
Isolated Nuclei ◽  
Messenger Ribonucleic Acid ◽  
Quantitative Changes

Cytoplasmic macromolecules were previously identified which regulate both qualitatively and quantitatively the release of messenger-like RNA from isolated nuclei. These macromolecules are now shown to be denatured at 45-50 degrees C and their synthesis is sensitive to pactamycin or cycloheximide. The putative regulatory proteins are essentially quantitatively precipitated with high specificity from the cytosol by streptomycin at a concentration 10-fold higher than that used to precipitate RNA. The nuclear concentration-dependence of RNA transport from successive samples of nuclei strongly suggests that the regulatory factors are recycled. Quantitative changes in the sequences transported at various dilutions of the cytosol suggest that not all the different classes of the putative regulatory macromolecules are present in an effective concentration at any one dilution.

scholarly journals Role of cytosol in the stimulation of RNA transport in vitro during cardiac hypertrophy in rats

1990 ◽  
Vol 267 (1) ◽  
pp. 133-140 ◽  
Author(s):  
A Subramaniam ◽  
M Thirunavukkarasu ◽  
C Rajamanickam
Keyword(s):  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Deae Cellulose ◽  
Gene Probe ◽  
Rna Transport ◽  
Transport Activity ◽  
Isolated Nuclei ◽  
Stimulation Of

The 100,000 g supernatant isolated from hypertrophic hearts on fractionation by (NH4)2SO4 and DEAE-cellulose chromatography showed an enhanced RNA-transport activity when incubated with isolated nuclei from sham-operated hearts in vitro. Proteins of Mr 73,000, 68,000, 43,000 and 32,000 are enriched in the DEAE-cellulose fractions exhibiting maximal transport activity, and they are phosphorylatable. Pretreatment of the cytosol with antibodies to the Mr-68,000 and -32,000 proteins decreases the transport activity of the cytosol from 14% to 4.25%. Proteins of Mr 73,000, 68,000, 43,000 and 32,000 are translocated from the cytosol to the nuclear envelope under conditions of RNA transport in vitro. Our results here suggest that at least two of these proteins, those of Mr 68,000 and 32,000, play an indispensible role in the nucleocytoplasmic RNA transport in vitro. By making use of a specific myosin heavy-chain B-gene probe and hybridization, we have also shown the effect of cytosol on the transport of myosin heavy-chain mRNA from nucleus to cytosol.

scholarly journals Importance of mammalian nuclear-envelope nucleoside triphosphatase in nucleo-cytoplasmic transport of ribonucleoproteins

1979 ◽  
Vol 182 (3) ◽  
pp. 811-819 ◽  
Author(s):  
P S Agutter ◽  
B McCaldin ◽  
H J McArdle
Keyword(s):  
Ionic Strength ◽  
Substrate Specificity ◽  
Nuclear Envelope ◽  
Nucleoside Triphosphate ◽  
Rna Transport ◽  
Isolated Nuclei ◽  
Cytoplasmic Rna ◽  
Cytoplasmic Transport ◽  
Nucleoside Triphosphatase

The nucleoside triphosphate-stimulated efflux of RNA from isolated nuclei was studied under a range of conditions, and the effects of these conditions on the process were compared with the properties of the nucleoside triphosphatase located in the pore complex. A marked similarity between the rate of efflux and the rate of nucleoside triphosphate hydrolysis was apparent, in terms of substrate specificity, sensitivity to treatment with insolubilized trypsin, kinetics and the effects of increased ionic strength and of many inhibitors. These results are taken, in view of earlier evidence, to suggest that the activity of the nucleoside triphosphatase is a prerequisite for nucleo-cytoplasmic RNA transport in vivo. There are some indications that the nuclear-envelope lipid is also involved in regulating the efflux process.

Some features of nucleo-cytoplasmic RNA transport from isolated nuclei

1981 ◽  
Vol 7 (1-3) ◽  
pp. 25-30 ◽  
Author(s):  
A. V. Peskin ◽  
Y. M. Koen ◽  
I. B. Zbarsky
Keyword(s):  
Rna Transport ◽  
Isolated Nuclei ◽  
Cytoplasmic Rna

scholarly journals RNA transport in isolated myeloma nuclei. Transport from membrane-denuded nuclei.

1977 ◽  
Vol 72 (1) ◽  
pp. 57-66 ◽  
Author(s):  
S E Stuart ◽  
G A Clawson ◽  
F M Rottman ◽  
R J Patterson
Keyword(s):  
Effect Of Temperature ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Rna Transport ◽  
Nuclear Membranes ◽  
Detergent Treatment ◽  
Pore Complexes ◽  
Triton X ◽  
Transport Of Macromolecules ◽  
Nonionic Detergents

Nuclei prepared from MOPC-21 cells were treated with the nonionic detergents Triton X-100 or Nonidet P-40. Chemical analysis revealed that nearly 90% of the nuclear phospholipid was removed by detergent treatment. The membrane-denuded nuclei remained intact with preservation of nuclear pore complexes as demonstrated by electron microscopy. Ribonucleic acid transport from detergent-treated nuclei proceeded at the same rate and to the same extent as in control nuclei. Normal nuclear restriction of nucleic acids was unaltered by removal of the nuclear membranes. The effect of temperature on transport of RNA from freshly isolated myeloma nuclei with intact nuclear envelopes was studied. No temperature transition was associated with the transport process. These data indicate that the transport of macromolecules from isolated myeloma nuclei is independent of the nuclear membrane.

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