High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae: a new vector for high-level expression

Gene ◽  
1989 ◽  
Vol 79 (2) ◽  
pp. 199-206 ◽  
Author(s):  
Teresa S. Lopes ◽  
Jacobus Klootwijk ◽  
Annemarie E. Veenstra ◽  
Paul C. van der Aar ◽  
Harm van Heerikhuizen ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ribosomal Dna ◽  
Copy Number ◽  
High Copy Number ◽  
High Level Expression ◽  
High Level

High-level secretion of correctly processed β-lactamase from Saccharomyces cerevisiae using a high-copy-number secretion vector

Gene ◽  
1994 ◽  
Vol 142 (1) ◽  
pp. 113-117 ◽  
Author(s):  
L.A. Castelli ◽  
C.J. Mardon ◽  
P.M. Strike ◽  
A.A. Azad ◽  
I.G. Macreadie
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Copy Number ◽  
High Copy Number ◽  
Secretion Vector ◽  
High Level

Mechanism of high-copy-number integration of pMIRY-type vectors into the ribosomal DNA of Saccharomyces cerevisiae

Gene ◽  
1991 ◽  
Vol 105 (1) ◽  
pp. 83-90 ◽  
Author(s):  
Teresa S. Lopes ◽  
Gert-Jan A.J. Hakkaart ◽  
Bert L. Koerts ◽  
Hendrik A. Raué ◽  
Rudi J. Planta
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ribosomal Dna ◽  
Copy Number ◽  
High Copy Number

scholarly journals Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation.

1989 ◽  
Vol 9 (4) ◽  
pp. 1488-1497 ◽  
Author(s):  
K W Runge ◽  
V A Zakian
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Sequences ◽  
Copy Number ◽  
Plasmid Copy Number ◽  
High Copy Number ◽  
Limiting Factor ◽  
Telomeric Dna ◽  
Base Pairs ◽  
Plasmid Copy ◽  
Telomere Elongation

The termini of Saccharomyces cerevisiae chromosomes consist of tracts of C1-3A (one to three cytosine and one adenine residue) sequences of approximately 450 base pairs in length. To gain insights into trans-acting factors at telomeres, high-copy-number linear and circular plasmids containing tracts of C1-3A sequences were introduced into S. cerevisiae. We devised a novel system to distinguish by color colonies that maintained the vector at 1 to 5, 20 to 50, and 100 to 400 copies per cell and used it to change the amount of telomeric DNA sequences per cell. An increase in the number of C1-3A sequences caused an increase in the length of telomeric C1-3A repeats that was proportional to plasmid copy number. Our data suggest that telomere growth is inhibited by a limiting factor(s) that specifically recognizes C1-3A sequences and that this factor can be effectively competed for by long tracts of C1-3A sequences at telomeres or on circular plasmids. Telomeres without this factor are exposed to processes that serve to lengthen chromosome ends.

High copy number integration into the ribosomal DNA of the yeast Phaffia rhodozyma

Gene ◽  
1997 ◽  
Vol 184 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Jan Wery ◽  
Diana Gutker ◽  
Anton C.H.M. Renniers ◽  
Jan C. Verdoes ◽  
Albert J.J. van Ooyen
Keyword(s):  
Ribosomal Dna ◽  
Copy Number ◽  
Phaffia Rhodozyma ◽  
High Copy Number
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